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IgG antibody

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F(ab')2 anti Human IgM Antibody - FITC thumbnail image 1
F(ab')2 anti Human IgM Antibody - FITC gallery image 1
Published customer image:
Goat F(ab′)2 anti Human IgG antibody (STAR97P) used for the detection of RA serum samples on citrullinated targets by western blotting.
Image caption:
Citrullination facilitates cross-reactivity of RF+ serum with fibrinogen in the absence of ACPAs.
(b) Native or citrullinated fibrinogen was probed using sera from the indicated RA patient cohorts or healthy controls.

From: Trela M, Perera S, Sheeran T, Rylance P, Nelson PN, Attridge K.
Citrullination facilitates cross-reactivity of rheumatoid factor with non-IgG1 Fc epitopes in rheumatoid arthritis.
Sci Rep. 2019 Aug 19;9(1):12068.
doi: 10.1038/s41598-019-48176-3.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.

Goat F(ab')2 anti Human IgG:FITC

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
IgG
Region
(H/L)

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Product Code Applications Pack Size List Price Your Price Qty
STAR97F
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F 0.7 mg loader
List Price Your Price
loader

Goat F(ab′)2 anti Human IgG antibody recognizes human IgG and demonstrates <1% cross-reactivity with other human immunoglobulin classes or subclasses.

Goat F(ab′)2 anti Human IgG antibody does not cross-react with Goat IgG F(c) or mouse, rat, bovine and horse serum proteins, as determined by immunoelectrophoresis.

Goat F(ab′)2 anti Human IgG antibody has been used for the detection of the antibody reponse to an endogenous retrovirus GAG matrix in patients with rheumatoid arthritis by ELISA (Nelson et al. 2014).

Target Species
Human
Product Form
F(ab')2 fragment of IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Antiserum Preparation
Antisera to human IgG were raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography. F(ab')2 fragments were prepared by pepsin digestion.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Immunogen
Human IgG.
Approx. Protein Concentrations
IgG concentration 0.7 mg/ml
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
FITC 490 525
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/50 1/100
Immunohistology - Frozen
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 50ul of the suggested working dilution to label 106 cells in 100ul.

Description Product Code Applications Pack Size List Price Your Price Quantity
Human Seroblock BUF070A F 50 Test
List Price Your Price
Description Human Seroblock
Human Seroblock BUF070B F 200 Test
List Price Your Price
Description Human Seroblock

References for IgG antibody

  1. Nelson, P.N. et al. (2014) Rheumatoid Arthritis is Associated with IgG Antibodies to Human Endogenous Retrovirus Gag Matrix: A Potential Pathogenic Mechanism of Disease?
    J Rheumatol. 41: 1952-60.
  2. Trela, M. et al. (2019) Citrullination facilitates cross-reactivity of rheumatoid factor with non-IgG1 Fc epitopes in rheumatoid arthritis.
    Sci Rep. 9 (1): 12068.

RRID
AB_322977
UniProt
P01857
P01834
P01860
P01859
P01861
P0CG04
Entrez Gene
IGKC
IGHG1
IGHG2
IGHG3
IGHG4
IGLV2-14
GO Terms
GO:0005515 protein binding
GO:0003823 antigen binding
GO:0005576 extracellular region
GO:0006958 complement activation, classical pathway
GO:0045087 innate immune response
GO:0005624 membrane fraction
GO:0006955 immune response

STAR97F

1602 162264 167486

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