IgE antibody
Goat anti Human IgE:Alk. Phos.
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- IgE
Goat anti Human IgE polyclonal antibody recognizes human IgE. Minimal cross-reactivity (<0.1%) is expected with other human immunoglobulins and light chains. Goat anti Human IgE in a biotinylated format has been successfully employed as a detection reagent in the development of a sensitive sandwich ELISA for the measurement of human IgE. |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Rhesus Monkey - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to Alkaline Phosphatase - liquid
- Antiserum Preparation
- Antiserum to human IgE was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- 50mM HEPES, 0.1M NaCl, 1mM MgCl2, 0.1mM ZnCl2
- Preservative Stabilisers
0.09% Sodium Azide 0.2% Bovine Serum Albumin - Immunogen
- Native human IgE.
- Approx. Protein Concentrations
- IgG concentration 0.5 mg/ml.
- Regulatory
- For research purposes only.
- Guarantee
- 12 months from date of despatch.
DO NOT FREEZE
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
ELISA | 1/1000 | 1/10000 | |
Western Blotting | 1/1000 | 1/5000 |
References for IgE antibody
-
Khan, F.M. et al. (2012) Basophil activation test compared to skin prick test and fluorescence enzyme immunoassay for aeroallergen-specific Immunoglobulin-E.
Allergy Asthma Clin Immunol. 8 (1): 1. -
Pinot de Moira, A. et al. (2013) Effects of treatment on IgE responses against parasite allergen-like proteins and immunity to reinfection in childhood schistosome and hookworm coinfections.
Infect Immun. 81 (1): 23-32. -
Zheng, W. et al. (2016) Upregulated expression of substance P in basophils of the patients with chronic spontaneous urticaria: induction of histamine release and basophil accumulation by substance P.
Cell Biol Toxicol. 32 (3): 217-28. -
Jin Y et al. (2015) Allergenic response to squid (Todarodes pacificus) tropomyosin Tod p1 structure modifications induced by high hydrostatic pressure.
Food Chem Toxicol. 76: 86-93. -
Wei, J.F. et al. (2009) Induction of mast cell accumulation, histamine release and skin edema by N49 phospholipase A2.
BMC Immunol. 10: 21. -
Tsai, C.T. et al. (2018) Isotype-specific agglutination-PCR (ISAP): A sensitive and multiplex method for measuring allergen-specific IgE.
J Allergy Clin Immunol. 141 (5): 1901-4.e15.
- RRID
- AB_1221598
- UniProt
- P01854
- Entrez Gene
- IGHE
- GO Terms
- GO:0003823 antigen binding
- GO:0006955 immune response
STAR147A
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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