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Lambda Light Chain antibody

Goat anti Human Lambda Light Chain:FITC

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
Lambda Light Chain

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STAR129F
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Goat anti Human Lambda Light Chain antibody recognizes human lambda light chains. This antibody has been cross absorbed with pooled human myeloma proteins bearing kappa light chains.

Target Species
Human
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Antiserum Preparation
Antisera to human lambda chains were raised by repeated immunisation of goats with purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Immunogen
Human lambda light chains.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
FITC 490 525
Regulatory
For research purposes only
Guarantee
Guaranteed for 12 months from the date of despatch or until the date of expiry, whichever comes first. Please see label for expiry date.

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/10
Immunofluorescence Neat 1/10
Immunohistology - Frozen
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

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References for Lambda Light Chain antibody

  1. Ben-horin, S. et al. (2016) Cross-immunogenicity: antibodies to infliximab in Remicade-treated patients with IBD similarly recognise the biosimilar Remsima.
    Gut. 65 (7): 1132-8.
  2. Magro, F. et al. (2018) The performance of Remicade®- optimized quantification assays in the assessment of Flixabi® levels.
    Therap Adv Gastroenterol. 11: 1756284818796956.

RRID
AB_1102725
UniProt
P0CG04
Entrez Gene
IGLV2-14
GO Terms
GO:0003823 antigen binding
GO:0005576 extracellular region
GO:0006958 complement activation, classical pathway
GO:0045087 innate immune response

View more products with LAMBDA LIGHT CHAIN specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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