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IgG antibody

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Anti Human IgG Gamma Chain Antibody thumbnail image 1
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Antigen down ELISA showing binding of Goat anti Human IgG gamma chain:HRP (STAR106P) to immobilized human IgG1 @ 5μgml-1.

Goat anti Human IgG Gamma Chain:HRP

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
IgG
Region
GAMMA CHAIN

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Product Code Applications Pack Size List Price Your Price Qty
STAR106P
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C E 0.8 mg loader
List Price Your Price
loader

Goat anti Human IgG gamma chain antibody recognizes human IgG and demonstrates <1% cross-reactivity with other human immunoglobulin classes or subclasses. Goat anti Human IgG gamma Chain antibody recognizes the Fc region of IgG, and has been adsorbed against mouse immunoglobulins to reduce species cross-reactivity.

Target Species
Human
Product Form
Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
Antiserum Preparation
Antisera to human IgG were raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01% Thiomersal
Immunogen
Human IgG.
Approx. Protein Concentrations
IgG concentration 0.8 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/3000
Immunohistology - Frozen
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

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AbGUARD® HRP Stabilizer Plus BUF052B C E P WB 500 ml
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AbGUARD® HRP Stabilizer Plus BUF052C C E P WB 1000 ml
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TMB Signal+ BUF054A E 100 ml
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TMB Core BUF056A E 100 ml
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References for IgG antibody

  1. Nielsen, L.S. et al. (2010) Single-batch production of recombinant human polyclonal antibodies.
    Mol Biotechnol. 45 (3): 257-66.
  2. Nordström, T. et al. (2011) Human Siglec-5 inhibitory receptor and immunoglobulin A (IgA) have separate binding sites in streptococcal beta protein.
    J Biol Chem. 286 (39): 33981-91.
  3. Hanitsch, L.G. et al. (2015) Late-Onset Disseminated Mycobacterium avium intracellulare Complex Infection (MAC), Cerebral Toxoplasmosis and Salmonella Sepsis in a German Caucasian Patient with Unusual Anti-Interferon-Gamma IgG1 Autoantibodies.
    J Clin Immunol. 35 (4): 361-5.
  4. Zheng, L. et al. (2015) Streptococcal 5′-nucleotidase A (S5nA), a novel group A Streptococcal virulence factor that facilitates immune evasion.
    J Biol Chem. pii: jbc.M115.677443.
  5. Zheng, L. et al. (2015) Streptococcal 5′-Nucleotidase A (S5nA), a Novel Streptococcus pyogenes Virulence Factor That Facilitates Immune Evasion.
    J Biol Chem. 290 (52): 31126-37.
  6. Le, Y. et al. (2017) Immunogenicity of murine mPEG-red blood cells and the risk of anti-PEG antibodies in human blood donors.
    Exp Hematol. 47: 36-47.e2.

Western Blotting

RRID
AB_323001
UniProt
P01857
P01859
P01860
P01861
Entrez Gene
IGHG1
IGHG2
IGHG3
IGHG4
GO Terms
GO:0005515 protein binding
GO:0003823 antigen binding
GO:0005576 extracellular region
GO:0005624 membrane fraction
GO:0006955 immune response

STAR106P

150563 155991

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