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IgG

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Purified Rabbit IgG:FITC

Product Type
Purified Protein
Isotype
Polyclonal IgG
Specificity
IgG

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Product Code Applications Pack Size List Price Your Price Qty
PRABP01F
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 0.1 mg loader
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loader

Rabbit immunoglobulin G is intended as a negative control reagent for use alongside specific rabbit IgG primary antibodies. It has been tested in flow cytometry on human tissues and demonstrates negligible binding.
Purified Rabbit immunoglobulin G may also be used as a standard for rabbit igG in ELISA (Blakney et al. 2021).

Target Species
Rabbit
Product Form
Purified polyclonal rabbit IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Preparation
Purified IgG prepared by ion exchange chromatography from normal rabbit serum
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
FITC 490 525
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry *
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. * It is recommended that the user dilutes the antibody for use in their own system to a concentration equivalent to their test reagent.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells or 100ul of whole blood

References for IgG

  1. Khalifeh, M.S. et al. (2010) Investigation of the role of tumour necrosis factor-{alpha}, interleukin-1{beta}, interleukin-10, nitric oxide and rheumatoid factor-immunoglobulin M in a rat model of arthritis.
    Lab Anim. 44: 143-9.
  2. Chang, J. et al. (2014) Hypoxia modulates the phenotype of osteoblasts isolated from knee osteoarthritis patients, leading to undermineralized bone nodule formation.
    Arthritis Rheumatol. 66 (7): 1789-99.
  3. Blakney, A.K. et al. (2021) Innate Inhibiting Proteins Enhance Expression and Immunogenicity of Self-Amplifying RNA.
    Mol Ther. 29 (3): 1174-85.

RRID
AB_324021
UniProt
P01870
Entrez Gene
LOC100009097
GO Terms
GO:0003823 antigen binding

PRABP01F

149809

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