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TNF Alpha

Recombinant Pig TNF Alpha

Product Type
Recombinant Protein
Specificity
TNF Alpha

Product Code Applications Pack Size List Price Your Price Qty
PPP029
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E WB 5 µg loader
List Price Your Price
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Recombinant Pig tumor necrosis factor alpha (TNF alpha), also known as tumor necrosis factor ligand superfamily member 2 (TNFSF2), corresponds to aa 77-232 of native pig TNF alpha. This recombinant protein is produced in yeast cells and is naturally folded and post translationally modified.

Pig TNF alpha is produced by a range of cells, including macrophages, monocytes, T and B lymphocytes and is an important mediator of the porcine inflammatory response.

This product may be used as a standard in immunoassays.

Target Species
Pig
Product Form
Purified recombinant protein - lyophilized
Reconstitution
Reconstitute with 20 μl sterile PBS containing 0.1% carrier protein
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Preparation
Recombinant protein expressed in Pichia pastoris and purified by ion-exchange chromatography
Source
Pichia pastoris
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
10% Trehalose
Purity
>95% by SDS PAGE analysis
Protein Molecular Weight
16.9 kDa
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Prior to reconstitution store at -20oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
ELISA
This reagent may be used as a standard in a sandwich ELISA assay in conjunction with AHP2397 as a coating antibody and AHP2397B as a detection antibody.

References for TNF Alpha

  1. Dawson, H.D. et al. (2020) Porcine cytokines, chemokines and growth factors: 2019 update.
    Res Vet Sci. 131: 266-300.

Synonyms
TNFSF2
UniProt
P23563
Entrez Gene
TNF
GO Terms
GO:0005125 cytokine activity
GO:0005615 extracellular space

PPP029

149198 153017 161241

If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.

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