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CD8 Beta antibody | 341

Mouse anti Rat CD8 Beta:FITC

Product Type
Monoclonal Antibody
Clone
341
Isotype
IgG1
Specificity
CD8 Beta

Product Code Applications Pack Size List Price Your Price Qty
MCA938F
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 0.1 mg loader
List Price Your Price
loader

Mouse anti Rat CD8 beta antibody, clone 341 recognizes the beta chain of the rat CD8 cell surface antigen, expressed on thymocytes and peripheral cytotoxic/suppressor cells. CD8b is not expressed by NK cells or by intestinal intraepithelial T cells. Mouse anti Rat CD8 beta antibody, clone 341 is reported to block proliferative and cytotoxic responses of CD8 +ve effector cells in vitro.

Target Species
Rat
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Immunogen
Rat/mouse T cell hybrids expressing CD8.
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Fusion Partners
Spleen cells from immunized BALB/c mice were fused with cells of the mouse X63Ag8.653 myeloma cell line.
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
FITC 490 525
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/2 1/10
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG1 Negative Control:FITC MCA1209F F 0.1 mg loader
>
List Price Your Price
loader
Description Mouse IgG1 Negative Control:FITC

References for CD8 Beta antibody

  1. Torres-Nagel, N. et al. (1992) Differential thymus dependence of rat CD8 isoform expression.
    Eur J Immunol. 22 (11): 2841-8.
  2. Yan, Y. et al. (2003) Pathogenesis of Autoimmunity After Xenogeneic Thymus Transplantation
    J Immunol. 170: 5936-46.
  3. Feferman, T. et al. (2005) Overexpression of IFN-induced protein 10 and its receptor CXCR3 in myasthenia gravis.
    J Immunol. 174 (9): 5324-31.
  4. Pérez-Cano, F.J. et al. (2010) Influence of breast milk polyamines on suckling rat immune system maturation.
    Dev Comp Immunol. 34: 210-8.
  5. Morales-Ferré, C. et al. (2022) Staphylococcus epidermidis' Overload During Suckling Impacts the Immune Development in Rats.
    Front Nutr. 9: 916690.
  6. Azagra-Boronat, I. et al. (2020) Lactobacillus fermentum CECT5716 Supplementation in Rats during Pregnancy and Lactation Impacts Maternal and Offspring Lipid Profile, Immune System and Microbiota.
    Cells. 9 (3): 575.

RRID
AB_321485
UniProt
P05541
Entrez Gene
Cd8b
GO Terms
GO:0016021 integral to membrane

MCA938F

152126

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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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