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CD49b antibody | AK7

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Anti Human CD49b Antibody, clone AK7 thumbnail image 1
Anti Human CD49b Antibody, clone AK7 gallery image 1
Figure A. FITC conjugated mouse anti human CD14 (MCA569F) and RPE conjugated Mouse IgG1 isotype control (MCA928PE). Figure B. FITC conjugated mouse anti human CD14 (MCA569F) and RPE conjugated mouse anti human CD49b (MCA743PE ). All experiments performed on red cell lysed Human peripheral blood gated on myeloid cells in the presence of Human SeroBlock (BUF070A).

Mouse anti Human CD49b:RPE

Product Type
Monoclonal Antibody
Clone
AK7
Isotype
IgG1
Specificity
CD49b

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Product Code Applications Pack Size List Price Your Price Qty
MCA743PE
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F 100 Tests loader
List Price Your Price
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Mouse anti Human CD49b antibody, clone AK7 recognizes the integrin alpha 2 subunit, a ~160 kDa glycoprotein that non-covalently associates with the ~130 kDa integrin beta 1 subunit to form the VLA-2 complex.

CD49b is expressed by platelets, long term cultivated T cells, approximately 50% of monocytes and most adherent cell lines.

Mouse anti Human CD49b antibody, clone AK7 inhibits cell attachment to collagen.

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Cynomolgus monkey
Rhesus Monkey
Baboon
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution
Reconstitute with 1 ml distilled water
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
5%Sucrose
Fusion Partners
Spleen cells from immunised mice were fused with cells of the mouse NS1 myeloma cell line.
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
RPE 488nm laser 496 578
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

DO NOT FREEZE.

This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood

How to Use the Spectraviewer

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  • Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
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  • Select the lasers and filters you wish to include
  • Select combined or multi-laser view to visualize the spectra

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG1 Negative Control:RPE MCA928PE F 100 Tests
>
List Price Your Price
Description Mouse IgG1 Negative Control:RPE

Description Product Code Applications Pack Size List Price Your Price Quantity
Human Seroblock BUF070A F 50 Test
List Price Your Price
Description Human Seroblock
Human Seroblock BUF070B F 200 Test
List Price Your Price
Description Human Seroblock

References for CD49b antibody

  1. Favaloro, E.J. et al. (1990) Co-expression of haemopoietic antigens on vascular endothelial cells: a detailed phenotypic analysis.
    Br J Haematol. 74 (4): 385-94.
  2. Mazurov, A.V. et al. (1991) A monoclonal antibody, VM64, reacts with a 130 kDa glycoprotein common to platelets and endothelial cells: heterogeneity in antibody binding to human aortic endothelial cells.
    Thromb Haemost. 66 (4): 494-9.
  3. Cavers, M. et al. (2002) Differential expression of beta1 and beta2 integrins and L-selectin on CD4+ and CD8+ T lymphocytes in human blood: comparative analysis between isolated cells, whole blood samples and cryopreserved preparations.
    Clin Exp Immunol. 127: 60-5.
  4. Koutsogiannaki, S. & Kaloyianni, M. (2011) Effect of 17β-estradiol on adhesion of Mytilus galloprovincialis hemocytes to selected substrates. Role of alpha2 integrin subunit.
    Fish Shellfish Immunol. 31 (1): 73-80.
  5. Hamaia, S.W. et al. (2012) Mapping of potent and specific binding motifs, GLOGEN and GVOGEA, for integrin α1β1 using collagen toolkits II and III.
    J Biol Chem. 287 (31): 26019-28.
  6. Mörtberg A et al. (2016) Sensitive detection of platelet-specific antibodies with a modified MAIPA using biotinylated antibodies and streptavidin-coated beads.
    J Immunol Methods. 434: 9-15.
  7. Rane, J.K. et al. (2016) Inhibition of the glucocorticoid receptor results in an enhanced miR-99a/100-mediated radiation response in stem-like cells from human prostate cancers.
    Oncotarget. 7 (32): 51965-80.
  8. García-gareta, E. et al. (2019) Engineering the migration and attachment behaviour of primary dermal fibroblasts.
    Biotechnol Bioeng. Jan 19 [Epub ahead of print].
    9. View The Latest Product References

Flow Cytometry

Synonyms
Integrin Alpha 2 Chain
VLA-2
RRID
AB_324702
UniProt
P17301
Entrez Gene
ITGA2
GO Terms
GO:0007596 blood coagulation
GO:0004872 receptor activity
GO:0005518 collagen binding
GO:0008305 integrin complex
GO:0007160 cell-matrix adhesion
GO:0007229 integrin-mediated signaling pathway
GO:0007411 axon guidance
GO:0042802 identical protein binding
GO:0044419 interspecies interaction between organisms

MCA743PE

147815

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