CD42b antibody | AK2

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry			Neat

Flow Cytometry

Use 10ul of the suggested working dilution to label 100ul whole blood.

Description	Product Code	Applications	Pack Size	List Price	Your Price	Quantity
Human Seroblock	BUF070A	F	50 Test		Log in	- +	Add to Cart Add to Quote Get Quote
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Description	Human Seroblock
Human Seroblock	BUF070B	F	200 Test		Log in	- +	Add to Cart Add to Quote Get Quote
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Description	Human Seroblock

References for CD42b antibody

1. Ward, C.M. & Berndt, M.C. (1995) Epitope and functional characterization of the CD42 (gpIb/IX) mAb panel.
   Leucocyte Typing V. White Cell Differentiation Antigens. Volume Two. Oxford University Press, Oxford.
2. Burgess, J.K. et al. (1998) Quinine-dependent antibodies bind a restricted set of epitopes on the glycoprotein Ib-IX complex: characterization of the epitopes.
   Blood. 92: 2366-73.
3. Burgess, J.K. et al. (2000) Rifampicin-dependent antibodies bind a similar or identical epitope to glycoprotein IX-specific quinine-dependent antibodies.
   Blood. 95: 1988-92.
4. Jayo, A. et al. (2010) L718P mutation in the membrane-proximal cytoplasmic tail of beta 3 promotes abnormal alpha IIb beta 3 clustering and lipid microdomain coalescence, and associates with a thrombasthenia-like phenotype.
   Haematologica. 95: 1158-66.
5. Lova, P. et al. (2004) Contribution of protease-activated receptors 1 and 4 and glycoprotein Ib-IX-V in the G(i)-independent activation of platelet Rap1B by thrombin.
   J Biol Chem. 279: 25299-306.
6. Shen, Y. et al. (2000) Requirement of leucine-rich repeats of glycoprotein (GP) Ibalpha for shear-dependent and static binding of von Willebrand factor to the platelet membrane GP Ib-IX-V complex.
   Blood. 95: 903-10.
7. Wright, S.D. et al. (1993) Double heterozygosity for mutations in the platelet glycoprotein IX gene in three siblings with Bernard-Soulier syndrome.
   Blood. 81: 2339-47.
8. Nomura, S. et al. (1995) Significance of cytokines and CD68-positive microparticles in immune thrombocytopenic purpura.
   Eur J Haematol. 55: 49-56.
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9. Speich, H.E. et al. (2008) Platelets undergo phosphorylation of Syk at Y525/526 and Y352 in response to pathophysiological shear stress.
   Am J Physiol Cell Physiol. 295: C1045-54.
10. Balduini, A. et al (2008) Adhesive receptors, extracellular proteins and myosin IIA orchestrate proplatelet formation by human megakaryocytes.
    J Thromb Haemost. 6: 1900-7.
11. Amor, N.B. et al. (2009) Acidic-store depletion is required for human platelet aggregation.
    Blood Coagul Fibrinolysis. 20: 511-6.
12. Tasneem, S. et al. (2009) Platelet adhesion to multimerin 1 in vitro: influences of platelet membrane receptors, von Willebrand factor and shear.
    J Thromb Haemost. 7: 685-92.
13. Lincoln, B. et al. (2010) Integrated system investigating shear-mediated platelet interactions with von Willebrand factor using microliters of whole blood
    Anal Biochem. 405: 174-83.
14. Goetzl, E.J. et al. (2016) Human plasma platelet-derived exosomes: effects of aspirin.
    FASEB J. 30 (5): 2058-63.
15. Michalska-Jakubus, M. et al. (2017) Plasma endothelial microparticles reflect the extent of capillaroscopic alterations and correlate with the severity of skin involvement in systemic sclerosis.
    Microvasc Res. 110: 24-31.
16. Ralph, A. et al. (2016) Computational Tracking of Shear-Mediated Platelet Interactions with von Willebrand Factor.
    Cardiovasc Eng Technol. 7 (4): 389-405.
17. Rossi, E. et al. (2018) Human endoglin as a potential new partner involved in platelet-endothelium interactions.
    Cell Mol Life Sci. 75 (7): 1269-84.
18. Kim, J.S. et al. (2021) Randomization to Omega-3 Fatty Acid Supplementation and Endothelial Function in COPD: The COD-Fish Randomized Controlled Trial.
    Chronic Obstr Pulm Dis. 8(1):41-53.
19. Yang, B. et al. (2023) Endothelial-Related Biomarkers in Evaluation of Vascular Function During Progression of Sepsis After Severe Trauma: New Potential Diagnostic Tools in Sepsis.
    J Inflamm Res. 16: 2773-82.

Flow Cytometry

Functional Assays

Synonyms

GPIB-ALPHA

RRID

AB_324071

UniProt

P07359

Entrez Gene

GP1BA

GO Terms

GO:0005515 protein binding

GO:0007155 cell adhesion

GO:0005887 integral to plasma membrane

GO:0005624 membrane fraction

GO:0007597 blood coagulation, intrinsic pathway

GO:0015057 thrombin receptor activity

GO:0030168 platelet activation

GO:0042730 fibrinolysis

MCA740B

150992 155744

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