GM-CSF antibody | E04-5B11
Mouse anti Human GM-CSF:Biotin
- Product Type
- Monoclonal Antibody
- Clone
- E04-5B11
- Isotype
- IgG1
- Specificity
- GM-CSF
Antibody Quality and Performance Guarantee
| Mouse anti Human GM-CSF antibody, clone E04-5B11, recognizes Granulocyte-Macrophage Colony-Stimulating Factor (GM-CSF) also known as colony stimulating factor 2. GM-CSF is a cytokine and acts as a hematopoietic grown factor and immune modulator of various immune cells. It is produced by immune cells including T cells and macrophages as well as endothelial and fibroblast cells. GM-CSF has various functions such as roles in recruiting circulating neutrophils, monocytes and lymphocytes (Shi et al. 2006). It is also involved in the maturation and activation of neutrophils and macrophages to induce microbial activity and the production of cytokines. GM-CSF elicits these functions through signaling via its receptor GM-CSFR to activate NF-kappaB or STAT5 signaling pathways (Dougan et al. 2019). The biotinylated Mouse anti Human GM-CSF antibody, clone E04-5B11 (MCA6231B) can be used as a detection antibody in a sandwich ELISA with the purified Mouse anti Human GM-CSF antibody, clone E01-1B6 (MCA6230GA) as the capture antibody. |
- Target Species
- Human
- Product Form
- Purified IgG conjugated to Biotin - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin - Immunogen
- E. coli. derived recombinant human GM-CSF (Ala18-Glu144)
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Fusion Partners
- Cell fusion between immunized BALB/c mouse spleen cells and mouse myeloma SP2/0
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
1.0 ug/ml |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse anti Human GM-CSF | MCA6230GA | E | 0.1 mg |
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| Description | Mouse anti Human GM-CSF | ||||||
- UniProt
- P04141
- Entrez Gene
- CSF2
- GO Terms
- GO:0005125 cytokine activity
- GO:0005129 granulocyte macrophage colony-stimulating factor receptor binding
- GO:0005615 extracellular space
- GO:0006955 immune response
- GO:0008083 growth factor activity
- GO:0010744 positive regulation of macrophage derived foam cell differentiation
- GO:0032747 positive regulation of interleukin-23 production
- GO:0042523 positive regulation of tyrosine phosphorylation of Stat5 protein
- GO:0045740 positive regulation of DNA replication
- View More GO Terms
- GO:0045918 negative regulation of cytolysis
MCA6231B
100005235
100006830
100008269
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
View more products with GM-CSF specificity
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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