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IL-17A/F antibody | I07-5E4

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Anti Human Interleukin-17A/F Antibody, clone I07-5E4 thumbnail image 1
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Sandwich ELISA analysis of human IL-17A/F using Mouse anti Human interleukin-17A/F (MCA6206GA) as a capture reagent and biotinylated Mouse anti Human interleukin-17A/F (MCA6207B) as a detection reagent with recombinant human IL-17A/F as the antigen. Detection was performed using HRP conjugated streptavidin and substrate.

Mouse anti Human Interleukin-17A/F:Biotin

Product Type
Monoclonal Antibody
Clone
I07-5E4
Isotype
IgG2a
Specificity
IL-17A/F

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Product Code Applications Pack Size List Price Your Price Qty
MCA6207B
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E 0.1 mg loader
List Price Your Price
loader

Mouse anti Human interleukin-17A/F antibody, clone I07-5E4, recognizes interleukin-17 A and F. The IL-17 family consists of six proteins (IL-17A to IL-17F) which function as proinflammatory cytokines, important for defence against pathogens (Gurczynski and Moore, 2017). Accordingly, IL-17-producing CD4+ T helper cells, also called Th17 cells, play important roles in immune defense. Uncontrolled IL-17 signaling is associated with immunopathology, autoimmune diseases, and cancer, and blocking activity of IL-17 can be beneficial in some clinical settings (Amatya et al. 2017). This antibody recognizes IL-17A, the most studied member of the IL-17 family, and its closest relative IL-17F (Chen and Kolls 2017). These two proteins have similar structure and inflammatory function, but unique functions of IL-17F in the immune system have also been indicated (Chang and Dong, 2009).

The biotinylated Mouse anti Human interleukin-17A/F antibody, clone I07-5E4 (MCA6207B) can be used as a detection antibody in a sandwich ELISA with the purified Mouse anti Human interleukin-17A/F antibody, clone D01-1C2 (MCA6206GA) as the capture antibody.

Target Species
Human
Product Form
Purified IgG conjugated to Biotin - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Immunogen
E. coli derived recombinant human full length interleukin-17A/F (Gly24-Ala155)
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Fusion Partners
Cell fusion between immunized BALB/c mouse spleen cells and mouse myeloma SP2/0
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1.0 ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse anti Human Interleukin-17A/F MCA6206GA E 0.1 mg loader
List Price Your Price
loader
Description Mouse anti Human Interleukin-17A/F

UniProt
Q16552
Q96PD4
Entrez Gene
IL17A
IL17F
GO Terms
GO:0001775 cell activation
GO:0006915 apoptosis
GO:0005125 cytokine activity
GO:0005615 extracellular space
GO:0006486 protein glycosylation
GO:0006954 inflammatory response
GO:0006955 immune response
GO:0007267 cell-cell signaling
GO:0032747 positive regulation of interleukin-23 production
GO:0045672 positive regulation of osteoclast differentiation
GO:0045944 positive regulation of transcription from RNA polymerase II promoter
GO:0006029 proteoglycan metabolic process
GO:0016525 negative regulation of angiogenesis
GO:0017015 regulation of transforming growth factor beta receptor signaling pathway
GO:0019955 cytokine binding
GO:0042109 lymphotoxin A biosynthetic process
GO:0042803 protein homodimerization activity
GO:0045076 regulation of interleukin-2 biosynthetic process
GO:0045408 regulation of interleukin-6 biosynthetic process
GO:0045414 regulation of interleukin-8 biosynthetic process
GO:0045423 regulation of granulocyte macrophage colony-stimulating factor biosynthetic process
GO:0051216 cartilage development

MCA6207B

100005095

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