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CD1 antibody | 76-7-4

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Anti Pig CD1 Antibody, clone 76-7-4 thumbnail image 1 Anti Pig CD1 Antibody, clone 76-7-4 thumbnail image 2
Anti Pig CD1 Antibody, clone 76-7-4 gallery image 1
Figure A. FITC conjugated Mouse anti Porcine CD14 (MCA1218F) and RPE conjugated Mouse IgG2a isotype control (MCA929PE). Figure B. FITC conjugated Mouse anti Porcine CD14 (MCA1218F) and RPE conjugated Mouse anti Porcine CD1 (MCA6098PE). All experiments performed on red cell lysed porcine blood gated on mononuclear cells in the presence of 10% pig serum. Data acquired on the ZE5 Cell Analyzer.
Anti Pig CD1 Antibody, clone 76-7-4 gallery image 2
Figure A. FITC conjugated Mouse anti Porcine CD45RA (MCA1751F) and RPE conjugated Mouse IgG2a isotype control (MCA929PE). Figure B. FITC conjugated Mouse anti Porcine CD45RA (MCA1751F) and RPE conjugated Mouse anti Porcine CD1 (MCA6098PE). All experiments performed on red cell lysed porcine blood gated on lymphocytes in the presence of 10% pig serum. Data acquired on the ZE5 Cell Analyzer.

Mouse anti Pig CD1:FITC

Product Type
Monoclonal Antibody
Clone
76-7-4
Isotype
IgG2a
Specificity
CD1

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Product Code Applications Pack Size List Price Your Price Qty
MCA6098F
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 0.1 mg loader
List Price Your Price
loader

Mouse anti Pig CD1 antibody, clone 76-7-4 recognizes a porcine homologue of the human CD1 cell surface receptor. CD1 is a member of the immunoglobulin superfamily. While it is structurally similar to the MHC class I receptor CD1 differs functionally by presenting lipid antigens to T cells (Germain & Margulies, 1993).

To date, five classes of the CD1 genes have been identified across different mammalian species, namely CD1A, CD1B, CD1C, CD1D, and CD1E (Brigl & Brenner, 2004). In swine various tissues express CD1C even though its gene has been reported to carry a loss-of-function mutation (Eguchi-Ogawa et al. 2007). CD1 is predominantly found on dendritic cells, macrophages, monocytes, and thymocytes (Chun et al. 1999).

Target Species
Pig
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Preparation
Purified IgG prepared by combination of precipitation and chromatography techniques
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Immunogen
Fresh dd miniature swine thymocytes
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
FITC 490 525
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG2a Negative Control:FITC MCA929F F 100 Tests loader
>
List Price Your Price
loader
Description Mouse IgG2a Negative Control:FITC

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse anti Pig CD45RA:RPE MCA1751PE F 100 Tests
List Price Your Price
Description Mouse anti Pig CD45RA:RPE

Source Reference

  1. Pescovitz, M.D. et al. (1984) Preparation and characterization of monoclonal antibodies reactive with porcine PBL.
    J Immunol. 133 (1): 368-75.

References for CD1 antibody

  1. Pauly, T. et al. (1998) Infection with classical swine fever virus: effects on phenotype and immune responsiveness of porcine T lymphocytes.
    J Gen Virol. 79 ( Pt 1): 31-40.

UniProt
Q9XS72
Entrez Gene
CD1.1
GO Terms
GO:0005886 plasma membrane
GO:0016021 integral to membrane
GO:0006955 immune response
GO:0010008 endosome membrane
GO:0019882 antigen processing and presentation

MCA6098F

150643 166661 180223

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