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CD34 antibody | QBEND/10

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Anti Human CD34 Antibody, clone QBEND/10 thumbnail image 1 Anti Human CD34 Antibody, clone QBEND/10 thumbnail image 2 Anti Human CD34 Antibody, clone QBEND/10 thumbnail image 3 Anti Human CD34 Antibody, clone QBEND/10 thumbnail image 4 Anti Human CD34 Antibody, clone QBEND/10 thumbnail image 5 Anti Human CD34 Antibody, clone QBEND/10 thumbnail image 6 Anti Human CD34 Antibody, clone QBEND/10 thumbnail image 7
Anti Human CD34 Antibody, clone QBEND/10 gallery image 1
Figure A. Alexa Fluor 647 conjugated Mouse anti Human CD45 (MCA87A647) and RPE conjugated Mouse IgG1 isotype control (MCA928PE) . Figure B. Alexa Fluor 647 conjugated Mouse anti Human CD45 (MCA87A647) and RPE conjugated Mouse anti Human CD34 (MCA547PE). All experiments performed on human blood gated on live, single mononuclear cells in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD34 Antibody, clone QBEND/10 gallery image 2
Figure A. Alexa Fluor 647 conjugated Mouse anti Human CD31 (MCA1738A647) and FITC conjugated Mouse IgG1 isotype control (MCA928F). Figure B. Alexa Fluor 647 conjugated Mouse anti Human CD31 (MCA1738A647) and FITC conjugated Mouse anti Human CD34 (MCA547F). All experiments performed on HUVEC cells gated on live single cells in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD34 Antibody, clone QBEND/10 gallery image 3
Figure A. Alexa Fluor 647 conjugated Mouse anti Human CD45 (MCA87A647) and RPE conjugated Mouse IgG1 isotype control (MCA928PE). Figure B. Alexa Fluor 647 conjugated Mouse anti Human CD45 (MCA87A647) and RPE conjugated Mouse anti Human CD34 (MCA547PE). All experiments performed on human peripheral blood lymphocytes in the presence of human SeroBlock (BUF070A).
Anti Human CD34 Antibody, clone QBEND/10 gallery image 4
Paraffin processed placenta stained with Mouse anti Human CD34 Class II (MCA547G).
Anti Human CD34 Antibody, clone QBEND/10 gallery image 5
Published customer image:
Mouse anti Human CD34 antibody, clone QBEND/10 (MCA547G) used for the detection of CD34 positive cells in vitro by immunofluorescence.
Image caption:
Characterization of human placental multipotent mesenchymal stromal cells (hPMSCs) after inducing differentiation into endothelial cells. Immunofluorescence staining of CD31 in undifferentiated hPMSCs is shown in (A) as a negative control. Immunofluorescence of endothelial markers in differentiated hPMSCs under induced culture conditions: (B) CD31, (C) CD34, (D) VE-cadherin, (E) VEGFR-1, (F) VEGFR-2, (G) von Willebrand factor, (H) von Willebrand factor under high magnification (Scale bar: 1 µm); Weibel-Palade bodies were visible within the differentiated hPMSC cytoplasm (arrow). CD105 was positive in (I) undifferentiated hPMSCs and (J) hPMSCs differentiated into endothelial cells. Cell nuclei were counterstained with DAPI. Scale bar: 10 µm. (K) Flow cytometry analysis of endothelial cell markers on the hPMSC surface before and after differentiation induced by VEGF-A. (L) mRNA for endothelial cell markers was amplified from 2 different strains of undifferentiated hPMSCs (lane 1 and 2) and hPMSCs differentiated into endothelial cells (lane 3 and 4). mRNA from human umbilical vein endothelial cells was used as a positive control (lane 5). The data shown are representative of 3 different experiments.

From: Lee M-Y, Huang J-P, Chen Y-Y, Aplin JD, Wu Y-H, et al.. (2009)
Angiogenesis in Differentiated Placental Multipotent Mesenchymal Stromal Cells Is Dependent on Integrin α5β1.
PLoS ONE 4(10): e6913.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Anti Human CD34 Antibody, clone QBEND/10 gallery image 6
Published customer image:
Mouse anti Human CD34 antibody, clone QBEND/10 (MCA547G) used to identify CD34 positive cells in cancer tissue microarrays by immunohistochemistry.
Image caption:
Examples of immunostained RCC group-specific markers CD34, DEK and MSH6. ccRCC with CD34-stained vascular microvessels (A, B); ccRCC with strong nuclear DEK (C) and MSH6 (D) positivity.

From: Beleut M, Zimmermann P, Baudis M, Bruni N, Bühlmann P, Laule O, Luu VD, Gruissem W, Schraml P, Moch H.
Integrative genome-wide expression profiling identifies three distinct molecular subgroups of renal cell carcinoma with different patient outcome.
BMC Cancer. 2012 Jul 23;12:310.
This image is from an open access article distributed under the terms of a Creative Commons Attribution License.
Anti Human CD34 Antibody, clone QBEND/10 gallery image 7
Published customer image:
Mouse anti Human CD34 antibody, clone QBEND/10 (MCA547G) used to label capillaries in melanoma biopsies by immunohistochemistry.
Image caption:
CD 34 staining demonstrating low (<20; A) and high (≥20; B) capillary density. Magnification ×40.

From: Maccio U, Mihic A, Lenggenhager D, Kolm I, Mittmann C, Heikenwälder M, Lorentzen A, Mihic-Probst D.
Hypoxia and Ezrin Expression in Primary Melanoma Have High Prognostic Relevance.
Int J Mol Sci. 2022 Sep 15;23(18):10745.
doi: 10.3390/ijms231810745.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.

Mouse anti Human CD34:RPE

Product Type
Monoclonal Antibody
Clone
QBEND/10
Isotype
IgG1
Specificity
CD34
Region
CLASS II

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Product Code Applications Pack Size List Price Your Price Qty
MCA547PET
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 25 Tests/0.25ml loader
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MCA547PE
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 100 Tests loader
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Mouse anti Human CD34 antibody, clone QBEND/10 recognizes the human CD34 antigen, also known as Hematopoietic progenitor cell antigen CD34. Human CD34 is 385 amino acid polypeptide containing a 31 residue signal peptide, cleaved to yield the ~110kDa mature form of CD34, a sialomucin single pass transmembrane glycoprotein. CD34 is expressed by stem cells (Kaufman et al. 2001) and small vessel endothelium (Ramani et al. 1990)

Human CD34 exists as two isoforms, the full length form described here and a truncated isoform lacking the carboxy-terminal of the intracellular domain and containing some alternative sequence in the remaining intracellular region. Antibody binding epitopes on human CD34 have been classified according to their resistance to enzymatic degradation and grouped together using this and competitive binding assays (Lanza et al. 1999). Mouse anti Human CD34 antibody, clone QBEND/10 has been classified as binding to the class II epitope, resistant to neuraminidase treatment but sensitive to both glycoprotease and chymopapain digestion. Mouse anti Human CD34 antibody, clone QBEND/10 binds to a different eoptope to Mouse anti Human CD34, clone 581 which binds to the class III epitope resistant to all three enzymzatic treatments (Nishio et al. 1996 In Leukocyte Typing VI). Clone QBEND 10 is expected to bind to both isoforms of human CD34 as it's binding epitope has been mapped to the extracellular domain between amino acids 43 and 49 by peptide microarray analysis (Jones et al. 1996, in Leukocyte Typing VI).

Mouse anti Human CD34 antibody, clone QBEND/10 has been successfully exploited for the detection of CD34 in brain capillaries of Alzheimer's patients (Kalaria et al. 1992) and in acute lymphoblastic leukemia cells (Sutherland et al. 1992) by western blotting.

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Bovine
Sheep
Rat
Dog
Cynomolgus monkey
Rhesus Monkey
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution
MCA547PET: Reconstitute with 0.25 ml distilled water
MCA547PE: Reconstitute with 1 ml distilled water
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
MCA547PET:
0.09%Sodium Azide
1%Bovine Serum Albumin
5%Sucrose
MCA547PE: 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
5% Sucrose
Immunogen
Human endothelial cell membrane vesicles.
Fusion Partners
Spleen cells from immunized NZB mice were fused with cells of the mouse NSO myeloma cell line.
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
RPE 488nm laser 496 578
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Pack Size: 25 Tests/0.25ml
Prior to reconstitution store at +4oC.
After reconstitution store at +4oC.
DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Pack Size: 100 Tests
Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

DO NOT FREEZE.

This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat Neat Pack Size: 25 Tests/0.25ml
1/10 Pack Size: 100 Tests
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in his/her own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1 x 106 cells in 100ul.

How to Use the Spectraviewer

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  • Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
  • Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
  • Select the lasers and filters you wish to include
  • Select combined or multi-laser view to visualize the spectra

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG1 Negative Control:RPE MCA928PE F 100 Tests
>
List Price Your Price
Description Mouse IgG1 Negative Control:RPE

Description Product Code Applications Pack Size List Price Your Price Quantity
Human Seroblock BUF070A F 50 Test
List Price Your Price
Description Human Seroblock
Human Seroblock BUF070B F 200 Test
List Price Your Price
Description Human Seroblock

References for CD34 antibody

  1. Fina, L. et al. (1990) Expression of the CD34 gene in vascular endothelial cells.
    Blood. 75 (12): 2417-26.
  2. Sauer, G. et al. (2003) Progression of cervical carcinomas is associated with down-regulation of CD9 but strong local re-expression at sites of transendothelial invasion.
    Clin Cancer Res. 9: 6426-31.
  3. Rutella, S. et al. (2003) Identification of a novel subpopulation of human cord blood CD34-CD133-CD7-CD45+lineage- cells capable of lymphoid/NK cell differentiation after in vitro exposure to IL-15.
    J Immunol. 171: 2977-88.
  4. Chan-Ling, T. et al. (2004) Astrocyte-endothelial cell relationships during human retinal vascular development.
    Invest Ophthalmol Vis Sci. 45: 2020-32.
  5. Zhao, M. et al. (2007) Evidence for the presence of stem cell-like progenitor cells in human adult pancreas.
    J Endocrinol. 195: 407-14.
  6. Jokubaitis, V.J. et al. (2008) Angiotensin-converting enzyme (CD143) marks hematopoietic stem cells in human embryonic, fetal, and adult hematopoietic tissues.
    Blood. 111: 4055-63.
  7. Lee, M.Y. et al. (2009) Angiogenesis in differentiated placental multipotent mesenchymal stromal cells is dependent on integrin alpha5beta1.
    PLoS One. 4: e6913.
  8. Chan-Ling T et al. (2011) Evidence of hematopoietic differentiation, vasculogenesis and angiogenesis in the formation of human choroidal blood vessels.
    Exp Eye Res. 92 (5): 361-76.
    9. View The Latest Product References
  9. Shetty, S. et al. (2011) Common lymphatic endothelial and vascular endothelial receptor-1 mediates the transmigration of regulatory T cells across human hepatic sinusoidal endothelium.
    J Immunol. 186: 4147-55.
  10. Chan-Ling T (2011) Role of CD44+ Stem Cells in Mural Cell Formation in the Human Choroid: Evidence of Vascular Instability Due to Limited Pericyte Ensheathment.
    Invest Ophthalmol Vis Sci. 52: 399-410.
  11. Beleut M et al. (2012) Integrative genome-wide expression profiling identifies three distinct molecular subgroups of renal cell carcinoma with different patient outcome.
    BMC Cancer. 12: 310.
  12. Suzuki, M. et al. (2012) Induction of human humoral immune responses in a novel HLA-DR-expressing transgenic NOD/Shi-scid/γcnull mouse.
    Int Immunol. 24 (4): 243-52.
  13. Hsieh, J.Y. et al. (2013) miR-146a-5p circuitry uncouples cell proliferation and migration, but not differentiation, in human mesenchymal stem cells.
    Nucleic Acids Res. 41 (21): 9753-63.
  14. Chen, S.P. et al. (2014) Reduced circulating endothelial progenitor cells in reversible cerebral vasoconstriction syndrome.
    J Headache Pain. 15: 82.
  15. Junaid TO et al. (2014) Fetoplacental vascular alterations associated with fetal growth restriction.
    Placenta. 35 (10): 808-15.
  16. Grognuz, A. et al. (2016) Human Fetal Progenitor Tenocytes for Regenerative Medicine.
    Cell Transplant. 25 (3): 463-79.
  17. Fan, C.Y. et al. (2017) De novo protein sequencing, humanization and in vitro effects of an antihuman CD34 mouse monoclonal antibody.
    Biochem Biophys Rep. 9: 51-60.
  18. Wang, D.Y. et al. (2017) Histological component quantification for the evaluation of endometrial receptivity in women with natural cycles undergoing in vitro fertilization/intracytoplasmic sperm injection.
    Taiwan J Obstet Gynecol. 56 (3): 368-70.
  19. GarikipatiV, N.S. et al. (2018) Isolation and characterization of mesenchymal stem cells from human fetus heart.
    PLoS One. 13 (2): e0192244.
  20. Rodewald, A.K. et al. (2019) Eight autopsy cases of melanoma brain metastases showing angiotropism and pericytic mimicry. Implications for extravascular migratory metastasis.
    J Cutan Pathol. 46 (8): 570-8.
  21. Maccio, U. et al. (2022) Hypoxia and Ezrin Expression in Primary Melanoma Have High Prognostic Relevance.
    Int J Mol Sci. 23 (18): 10745.
  22. Weible, M.I. et al. (2024) BMPRII(+) neural precursor cells isolated and characterized from organotypic neurospheres: an in vitro model of human fetal spinal cord development.
    Neural Regen Res. 19 (2): 447-57.
  23. Tripathy, N.K. et al. (2018) Cardiomyogenic Heterogeneity of Clonal Subpopulations of Human Bone Marrow Mesenchymal Stem Cells.
    J Stem Cells Regen Med. 14 (1): 27-33.
  24. Hill, W. et al. (2024) Late transplant-associated thrombotic microangiopathy verified in bone marrow biopsy specimens is associated with chronic GVHD and viral infections.
    Eur J Haematol. Jan 20 [Epub ahead of print].

Further Reading

  1. Gorr, T.A. et al. (2011) Old proteins - new locations: myoglobin, haemoglobin, neuroglobin and cytoglobin in solid tumours and cancer cells.
    Acta Physiol (Oxf). 202: 563-581.

Flow Cytometry

Immunofluorescence

RRID
AB_1125257
UniProt
P28906
Entrez Gene
CD34
GO Terms
GO:0016021 integral to membrane
GO:0030246 carbohydrate binding
GO:0009897 external side of plasma membrane
GO:0016337 cell-cell adhesion
GO:0050776 regulation of immune response
GO:0050900 leukocyte migration

MCA547PET

1704

MCA547PE

1604 167757

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