CD45RC antibody | OX-22
Mouse anti Rat CD45RC:Low Endotoxin
- Product Type
- Monoclonal Antibody
- Clone
- OX-22
- Isotype
- IgG1
- Specificity
- CD45RC
Mouse anti Rat CD45RC antibody, clone OX-22 recognizes rat CD45RC, the high molecular weight form of the leucocyte common antigen. The antigen is found on B cells, approximately 50% of bone marrow cells, all CD8+ve T cells, but splits CD4+ve T cells into two populations, CD4+CD45RChigh (Th1-like) and CD4+CD45RClow (Th2-like). This product is routinely tested in flow cytometry on rat splenocytes. |
- Target Species
- Rat
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Carrier Free
- Yes
- Immunogen
- Phytohaemagglutinin (PHA) -activated rat lymphocytes
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Endotoxin Level
- <0.01 EU/ug
- Fusion Partners
- Spleen cells from immunized BALB/c mice were fused with cells from the NS1 mouse myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | 1/100 | 1/200 | |
Immunohistology - Frozen | |||
Immunohistology - Paraffin 1 |
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
---|---|---|---|---|---|---|---|
Mouse IgG1 Negative Control:Low Endotoxin | MCA1209EL | F | 0.5 mg | Log in | |||
List Price | Your Price | ||||||
Log in | |||||||
Description | Mouse IgG1 Negative Control:Low Endotoxin |
Source Reference
-
Spickett, G.P. et al. (1983) MRC OX-22, a monoclonal antibody that labels a new subset of T lymphocytes and reacts with the high molecular weight form of the leukocyte-common antigen.
J Exp Med. 158 (3): 795-810.
References for CD45RC antibody
-
Arthur, R.P. & Mason, D. (1986) T cells that help B cell responses to soluble antigen are distinguishable from those producing interleukin 2 on mitogenic or allogeneic stimulation.
J Exp Med. 163 (4): 774-86. -
Pelegrí, C. et al. (2001) Prevention of adjuvant arthritis by the W3/25 anti-CD4 monoclonal antibody is associated with a decrease of blood CD4(+)CD45RC(high) T cells.
Clin Exp Immunol. 125 (3): 470-7. -
Mueller, C.A. et al. (2003) Spinal cord injury induces lesional expression of the proinflammatory and antiangiogenic cytokine EMAP II.
J Neurotrauma. 20 (10): 1007-15. -
Fulgenzi, A. et al. (2004) Distribution of 99mTc-labeled lymphocytes in control and inflamed rats.
Nucl Med Biol. 31 (5): 631-8. -
Schwab, J.M. et al. (2005) Spinal cord injury induces early and persistent lesional P2X4 receptor expression.
J Neuroimmunol. 163 (1-2): 185-9. -
Schwab, J.M. et al. (2005) Spinal cord injury-induced lesional expression of the repulsive guidance molecule (RGM).
Eur J Neurosci. 21 (6): 1569-76. -
Conrad, S. et al. (2005) Prolonged lesional expression of RhoA and RhoB following spinal cord injury.
J Comp Neurol. 487 (2): 166-75. -
Herrero-Fresneda, I. et al. (2005) Reduction of postischemic immune inflammatory response: an effective strategy for attenuating chronic allograft nephropathy.
Transplantation. 79 (2): 165-73.
View The Latest Product References
-
Mueller, C.A. et al. (2007) Lesional expression of the endogenous angiogenesis inhibitor endostatin/collagen XVIII following traumatic brain injury (TBI).
Exp Neurol. 208 (2): 228-37. -
Adzemovic, M.V. et al. (2013) Imatinib ameliorates neuroinflammation in a rat model of multiple sclerosis by enhancing blood-brain barrier integrity and by modulating the peripheral immune response.
PLoS One. 8 (2): e56586. -
Xu, L. et al. (2019) Natural Diterpenoid Oridonin Ameliorates Experimental Autoimmune Neuritis by Promoting Anti-inflammatory Macrophages Through Blocking Notch Pathway.
Front Neurosci. 13: 272.
- RRID
- AB_2174432
- UniProt
- P04157
- Entrez Gene
- Ptprc
- GO Terms
- GO:0001915 negative regulation of T cell mediated cytotoxicity
- GO:0001960 negative regulation of cytokine-mediated signaling pathway
- GO:0005001 transmembrane receptor protein tyrosine phosphatase activity
- GO:0005887 integral to plasma membrane
- GO:0005925 focal adhesion
- GO:0006469 negative regulation of protein kinase activity
- GO:0006470 protein dephosphorylation
- GO:0019887 protein kinase regulator activity
- GO:0009898 internal side of plasma membrane
- View More GO Terms
- GO:0010332 response to gamma radiation
- GO:0019901 protein kinase binding
- GO:0042100 B cell proliferation
- GO:0045121 membrane raft
- GO:0050852 T cell receptor signaling pathway
- GO:0050853 B cell receptor signaling pathway
- GO:0050857 positive regulation of antigen receptor-mediated signaling pathway
- GO:0051209 release of sequestered calcium ion into cytosol
- GO:0051607 defense response to virus
MCA53EL
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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