CD235a antibody | YTH89.1
Rat anti Human CD235a:Alexa Fluor® 647
- Product Type
- Monoclonal Antibody
- Clone
- YTH89.1
- Isotype
- IgG2b
- Specificity
- CD235a
Antibody Quality and Performance Guarantee
| Rat anti Human CD235a antibody, clone YTH89.1 recognizes glycophorin A, a major sialoglycoprotein of the human erythrocyte membrane. |
- Target Species
- Human
- Product Form
- Purified IgG conjugated to Alexa Fluor® 647 - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Fusion Partners
- Spleen cells from immunized DA rats were fused with cells of the Y3/Ag.1.2.3.
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) Alexa Fluor®647 650 665 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
Neat |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Rat IgG2b Negative Control:Alexa Fluor® 647 | MCA6006A647 | F | 100 Tests/1ml |
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Log in | ||
| List Price | Your Price | ||||||
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| Description | Rat IgG2b Negative Control:Alexa Fluor® 647 | ||||||
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Human Seroblock | BUF070A | F | 50 Test | Log in | |||
| List Price | Your Price | ||||||
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| Description | Human Seroblock | ||||||
| Human Seroblock | BUF070B | F | 200 Test | Log in | |||
| List Price | Your Price | ||||||
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| Description | Human Seroblock | ||||||
References for CD235a antibody
-
Outram, S. et al. (1988) Erythromyeloid lineage fidelity is conserved in erythroleukaemia.
Leuk Res. 12 (8): 651-7. -
Jokiranta, T.S. & Meri, S. (1993) Biotinylation of monoclonal antibodies prevents their ability to activate the classical pathway of complement.
J Immunol. 151 (4): 2124-31. -
Hoang, T. et al. (1996) Opposing effects of the basic helix-loop-helix transcription factor SCL on erythroid and monocytic differentiation.
Blood. 87: 102-11. -
Babiker, A.A. et al. (2002) Transfer of prostasomal CD59 to CD59-deficient red blood cells results in protection against complement-mediated hemolysis.
Am J Reprod Immunol. 47 (3): 183-92. -
Lahlil, R. et al. (2004) SCL assembles a multifactorial complex that determines glycophorin A expression.
Mol Cell Biol. 24: 1439-52. -
Challier, J.C. et al. (2005) Immunocytological evidence for hematopoiesis in the early human placenta.
Placenta. 26: 282-8. -
Huang, Y.C. et al. (2009) Oral small-molecule tyrosine kinase inhibitor midostaurin (PKC412) inhibits growth and induces megakaryocytic differentiation in human leukemia cells.
Toxicol In Vitro. 23: 979-85. -
Tiziani, S. et al. (2009) Metabolomic profiling of drug responses in acute myeloid leukaemia cell lines.
PLoS One. 2009;4(1):e4251.
View The Latest Product References
-
Basu, S.
(2010) Erythrocyte membrane defects and asymmetry in paroxysmal nocturnal hemoglobinuria and myelodysplastic syndrome.
Hematology. 15: 236-9. -
Saha, S. et al. (2011) Elevated levels of redox regulators, membrane-bound globin chains, and cytoskeletal protein fragments in hereditary spherocytosis erythrocyte proteome.
Eur J Haematol. 87: 259-66. -
Lucky AB et al. (2016) Plasmodium knowlesi Skeleton-Binding Protein 1 Localizes to the 'Sinton and Mulligan' Stipplings in the Cytoplasm of Monkey and Human Erythrocytes.
PLoS One. 11 (10): e0164272. -
Scanlon, V.M. et al. (2022) Multiparameter analysis of timelapse imaging reveals kinetics of megakaryocytic erythroid progenitor clonal expansion and differentiation.
Sci Rep. 12 (1): 16218.
- Synonyms
- Glycophorin A
- RRID
- AB_566660
- UniProt
- P02724
- Entrez Gene
- GYPA
- GO Terms
- GO:0005886 plasma membrane
- GO:0016021 integral to membrane
- GO:0005624 membrane fraction
- GO:0042802 identical protein binding
- GO:0044419 interspecies interaction between organisms
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