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MHC Class I H-2Kb antibody | AF6-88.5

Mouse anti Mouse MHC Class I H-2Kb:FITC

Product Type
Monoclonal Antibody
Clone
AF6-88.5
Isotype
IgG2a
Specificity
MHC Class I H-2Kb

Product Code Applications Pack Size List Price Your Price Qty
MCA4731F
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 0.1 mg loader
List Price Your Price
loader

Mouse anti Mouse MHC Class I H-2Kb antibody, clone AF6-88.5 recognizes the H-2Kb determinant present on all nucleated cells of mice expressing this MHC class I haplotype.

The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In mice, this complex is referred to as the histocompatibility 2 (H-2) region. There are 3 major MHC class I proteins encoded by the H-2 which are H-2K, H-2L and H-2D. The H- 2K gene is part of the murine H-2 complex on chromosome 17 and there are a large number of variant alleles of this gene.

Mouse anti Mouse MHC Class I H-2Kb antibody, clone AF6-88.5 does not cross react with other haplotypes.

Target Species
Mouse
Product Form
Purified IgG conjugated to fluorescein isothiocyanate (FITC) - liquid.
Preparation
Purified IgG prepared by affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Immunogen
C57BL mouse splenocytes.
Approx. Protein Concentrations
IgG concentration 0.5mg/ml
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
FITC 490 525
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/10 1/20
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse Seroblock FcR BUF041A F 0.1 mg loader
List Price Your Price
loader
Description Mouse Seroblock FcR
Mouse Seroblock FcR BUF041B F 0.5 mg loader
List Price Your Price
loader
Description Mouse Seroblock FcR

References for MHC Class I H-2Kb antibody

  1. Mareeva, T. et al. (2010) A novel composite immunotoxin that suppresses rabies virus production by the infected cells.
    J Immunol Methods. 353 (1-2): 78-86.

Flow Cytometry

RRID
AB_1605302
UniProt
P01901
Entrez Gene
H2-K1
GO Terms
GO:0001916 positive regulation of T cell mediated cytotoxicity
GO:0002485 antigen processing and presentation of endogenous peptide antigen via MHC class I via ER pathway, TAP-dependent
GO:0042742 defense response to bacterium
GO:0042612 MHC class I protein complex
GO:0016021 integral to membrane
GO:0042605 peptide antigen binding
GO:0006955 immune response
GO:0009897 external side of plasma membrane
GO:0042590 antigen processing and presentation of exogenous peptide antigen via MHC class I

MCA4731F

159529 159937 160927

If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.

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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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