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Troponin T antibody | T1/61

Mouse anti Chicken Troponin T

Product Type
Monoclonal Antibody
Clone
T1/61
Isotype
IgG1
Specificity
Troponin T

Product Code Applications Pack Size List Price Your Price Qty
MCA470
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C E IF P WB 0.25 mg loader
List Price Your Price
loader

Mouse anti Chicken Troponin T antibody, clone T1/61 recognizes chicken Troponin T, fast skeletal muscle isoforms, also known as fast muscle Troponin T. Troponin T is a 262 amino acid tropomyosin binding component of the troponin complex with a predicted molecular mass of ~31 kDa.

Four isoforms of Troponin T generated by alternative splicing have been described. Mouse anti Chicken Troponin T antibody, clone T1/61 recognizes the canonical isoform 1, binding to othe potential isoforms has not been evaluated (UniProt : P12620).

Target Species
Chicken
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rabbit
Human
Quail
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
Troponin T, purified from chicken breast muscle
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Immunofluorescence
Immunohistology - Frozen
Immunohistology - Paraffin
Western Blotting
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Immunohistology
This product does not require protein digestion pre-treatment of paraffin sections.This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections.
Histology Positive Control Tissue
Skeletal muscle

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Source Reference

  1. Bird, I.M. et al. (1985) Identification of multiple variants of fast muscle troponin T in the chicken using monoclonal antibodies.
    Eur J Biochem. 150 (3): 517-25.

References for Troponin T antibody

  1. (2011) Human Protein Atlas: CAB000472
    Human Protein Atlas: CAB000472
  2. Watkins, H. et al. (1996) Expression and functional assessment of a truncated cardiac troponin T that causes hypertrophic cardiomyopathy. Evidence for a dominant negative action.
    J Clin Invest. 98: 2456-61.
  3. Marian, A.J. et al. (1997) Expression of a mutant (Arg92Gln) human cardiac troponin T, known to cause hypertrophic cardiomyopathy, impairs adult cardiac myocyte contractility.
    Circ Res. 81: 76-85.
  4. Sweeney, H.L. et al. (1998) Functional analyses of troponin T mutations that cause hypertrophic cardiomyopathy: insights into disease pathogenesis and troponin function.
    Proc Natl Acad Sci U S A. 95: 14406-10.
  5. Herman, E.H. et al. (1999) Correlation between serum levels of cardiac troponin-T and the severity of the chronic cardiomyopathy induced by doxorubicin.
    J Clin Oncol. 17: 2237-43.
  6. Dunnick, J.K. et al. (2004) Cardiac damage in rodents after exposure to bis(2-chloroethoxy)methane.
    Toxicol Pathol. 32: 309-17.
  7. Zhang, J. et al. (2006) Mechanisms and biomarkers of cardiovascular injury induced by phosphodiesterase inhibitor III SK&F 95654 in the spontaneously hypertensive rat.
    Toxicol Pathol. 34: 152-63.
  8. Zhang, J. et al. (2008) Isoproterenol-induced cardiotoxicity in sprague-dawley rats: correlation of reversible and irreversible myocardial injury with release of cardiac troponin T and roles of iNOS in myocardial injury.
    Toxicol Pathol. 36: 277-8.
  9. View The Latest Product References

Immunohistology - Paraffin

Western Blotting

RRID
AB_2206754
UniProt
P12620
Entrez Gene
TNNT3

MCA470

159202

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