CD266 antibody | ITEM-4
Mouse anti Human CD266:FITC
- Product Type
- Monoclonal Antibody
- Clone
- ITEM-4
- Isotype
- IgG2b
- Specificity
- CD266
Mouse anti Human CD266 antibody, clone ITEM-4 recognizes human tumor necrosis factor receptor superfamily member 12A, also known as CD266, Fibroblast growth factor-inducible immediate-early response protein 14 or Tweak-receptor. CD266 is a 102 amino acid ~14 kDa type I transmembrane glycoprotein and member of the tumour necrosis factor receptor superfamily (TNFRSF12A) expressed by a variety of tissues, including the heart, lung and kidney, and at elevated levels by human brain and liver tumors. The interaction between CD266 and its ligand CD255 (TWEAK), induces the proliferation and migration of endothelial cells and also angiogenesis, and CD255-induced apoptosis of some tumor cell lines is mediated by CD266 (Nakayama et al. 2003). Mouse anti Human CD266 antibody, clone ITEM-4 is cross-reactive with mouse CD266, and also with Rat-1 fibroblastoid cells in flow cytometry. |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline pH7.4
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin - Immunogen
- Human CD266 transfected P815 cells.
- Approx. Protein Concentrations
- IgG concentration 0.1mg/ml
- Fusion Partners
- Spleen cells from immunized BALB/c mice were fused with cells of the P3U1 mouse myeloma cell line.
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) FITC 490 525 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry |
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
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Mouse IgG2b Negative Control:FITC | MCA691F | F | 100 Tests | Log in | |||
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Description | Mouse IgG2b Negative Control:FITC |
Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
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Human Seroblock | BUF070A | F | 50 Test | Log in | |||
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Description | Human Seroblock | ||||||
Human Seroblock | BUF070B | F | 200 Test | Log in | |||
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Description | Human Seroblock |
References for CD266 antibody
-
Nakayama, M. et al. (2003) Fibroblast growth factor-inducible 14 mediates multiple pathways of TWEAK-induced cell death.
J Immunol. 170 (1): 341-8. -
Nakayama, M. et al. (2003) Characterization of murine TWEAK and its receptor (Fn14) by monoclonal antibodies.
Biochem Biophys Res Commun. 306 (4): 819-25. -
Girgenrath, M. et al. (2006) TWEAK, via its receptor Fn14, is a novel regulator of mesenchymal progenitor cells and skeletal muscle regeneration.
EMBO J. 25: 5826-39.
- Synonyms
- TWEAK-R
- RRID
- AB_10851349
- UniProt
- Q9NP84
- Entrez Gene
- TNFRSF12A
- GO Terms
- GO:0001525 angiogenesis
- GO:0006915 apoptosis
- GO:0016021 integral to membrane
- GO:0004872 receptor activity
MCA4699F
0509R 155272If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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