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CD8 antibody | RPA-T8

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Anti Human CD8 Antibody, clone RPA-T8 thumbnail image 1 Anti Human CD8 Antibody, clone RPA-T8 thumbnail image 2
Anti Human CD8 Antibody, clone RPA-T8 gallery image 1
Figure A. RPE conjugated Mouse anti Human CD3 (MCA2184PE) and purified Mouse IgG1 isotype control (MCA928) detected with Goat anti Mouse IgG1 FITC (STAR132). Figure B. RPE conjugated Mouse anti Human CD3 (MCA2184PE) and purified Mouse anti Human CD8 (MCA4609T) detected with Goat anti Mouse IgG1 FITC (STAR132). All experiments performed on human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer
Anti Human CD8 Antibody, clone RPA-T8 gallery image 2
Figure A. Alexa Fluor 700 conjugated Mouse anti Human CD3 (MCA463A700) and purified Mouse IgG1 isotype control (MCA928) detected with Goat anti Mouse IgG PE (STAR76PE). Figure B. Alexa Fluor 700 conjugated Mouse anti Human CD3 (MCA463A700) and purified Mouse anti Human CD8 (MCA4609T) detected with Goat anti Mouse IgG PE (STAR76PE). All experiments performed on red cell lysed human blood gated on lymphoid cells in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.

Mouse anti Human CD8:FITC

Product Type
Monoclonal Antibody
Clone
RPA-T8
Isotype
IgG1
Specificity
CD8

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Product Code Applications Pack Size List Price Your Price Qty
MCA4609F
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 0.1 mg loader
List Price Your Price
loader

Mouse anti Human CD8 monoclonal antibody, clone RPA-T8 recognizes the alpha-chain of the human CD8 antigen, a ~32 kDa single pass type 1 membrane glycoprotein containing a single Ig-like V-type domain. CD8α forms either homodimers or disulphide bond linked heterodimers with the CD8β chain. CD8 is expressed by thymocytes, the cytotoxic-suppressor subset of T cells and weakly by NK cells. CD8 functions as a receptor for MHC Class I molecules.

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Chimpanzee
Baboon
Cynomolgus monkey
Rhesus Monkey
Macaque
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Preparation
Purified IgG prepared by affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
FITC 490 525
Regulatory
For research purposes only
Guarantee
Guaranteed for 12 months from the date of despatch or until the date of expiry, whichever comes first. Please see label for expiry date.

Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/5 1/10
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1 x 106 cells or cells or 100ul human whole blood

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG1 Negative Control:FITC MCA928F F 100 Tests loader
>
List Price Your Price
loader
Description Mouse IgG1 Negative Control:FITC

References for CD8 antibody

  1. Morbius, U. (1989) Cluster Report: CD8
    inLeukocyte Typing IV, White Cell Differentiation Antigens, edited by Knapp W, Dorken B, Gilks WR, Rieber E, Schmidt RE, Stein H, Von Dem Bome AEG, Oxford University Press: pp 475-8.
  2. Suranyi, M.G. et al. (1991) Lymphocyte adhesion molecules in T cell-mediated lysis of human kidney cells.
    Kidney Int. 39 (2): 312-9.
  3. Aversa, G. et al. (1994) A monoclonal antibody (A6) recognizing a unique epitope restricted to CD45RO and RB isoforms of the leukocyte common antigen family identifies functional T cell subsets.
    Cell Immunol. 158 (2): 314-28.
  4. SanSebastian, W. et al. (2013) Adeno-associated virus type 6 is retrogradely transported in the non-human primate brain.
    Gene Ther. 20 (12): 1178-83.
  5. Samaranch, L. et al. (2014) AAV9-mediated expression of a non-self protein in nonhuman primate central nervous system triggers widespread neuroinflammation driven by antigen-presenting cell transduction.
    Mol Ther. 22 (2): 329-37.

UniProt
P01732
P10966
Entrez Gene
CD8A
CD8B
GO Terms
GO:0005576 extracellular region
GO:0007169 transmembrane receptor protein tyrosine kinase signaling pathway
GO:0005887 integral to plasma membrane
GO:0015026 coreceptor activity
GO:0019882 antigen processing and presentation
GO:0042101 T cell receptor complex
GO:0042288 MHC class I protein binding
GO:0050776 regulation of immune response

MCA4609F

153753

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