CD25 antibody | OX-39
Mouse anti Rat CD25:Alexa Fluor® 647
- Product Type
- Monoclonal Antibody
- Clone
- OX-39
- Isotype
- IgG1
- Specificity
- CD25
Mouse anti Rat CD25 antibody, clone OX-39 recognizes the alpha chain of rat CD25, otherwise known as IL-2 receptor alpha, a ~55 kDa type I membrane glycoprotein, expressed by activated T cells but not resting lymphocytes. CD25 is also expressed by dendritic cells found in the thymus medulla. Mouse anti Rat CD25 antibody, clone OX-39 has been described reacting with paraffin-embedded material following PLP fixation (periodate-lysine-paraformaldehyde). Mouse anti Rat CD25 antibody, clone OX-39 has been shown to weakly inhibit the binding of IL-2 to Con-A stimulated spleen blasts (Paterson et al. 1987). |
- Target Species
- Rat
- Product Form
- Purified IgG conjugated to Alexa Fluor® 647 - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Immunogen
- Stimulated Rat T cells
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Fusion Partners
- Spleen cells from immunized BALB/c mice were fused with cells of the NS0/1 mouse myeloma cell line.
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) Alexa Fluor®647 650 665 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | Neat | 1/5 |
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
---|---|---|---|---|---|---|---|
Mouse IgG1 Negative Control:Alexa Fluor® 647 | MCA1209A647 | F | 100 Tests/1ml | Log in | |||
List Price | Your Price | ||||||
Log in | |||||||
Description | Mouse IgG1 Negative Control:Alexa Fluor® 647 |
References for CD25 antibody
-
Paterson, D.J. et al. (1987) Antigens of activated rat T lymphocytes including a molecule of 50,000 Mr detected only on CD4 positive T blasts.
Mol Immunol. 24 (12): 1281-90. -
Charteris DG & Lightman SL (1993) In vivo lymphokine production in experimental autoimmune uveoretinitis.
Immunology. 78 (3): 387-92. -
Hayosh, N.S. & Swanborg, R.H. (1987) Autoimmune effector cells. IX. Inhibition of adoptive transfer of autoimmune encephalomyelitis with a monoclonal antibody specific for interleukin 2 receptors.
J Immunol. 138 (11): 3771-5. -
Tellides, G. et al. (1987) Functional blocking of the interleukin-2 receptor (IL-2R) may be important in the efficacy of IL-2R antibody therapy.
Transplant Proc. 19 (5): 4231-3. -
Signore, A. et al. (1987) Detection of activated lymphocytes in endocrine pancreas of BB/W rats by injection of 123I-interleukin-2: an early sign of type 1 diabetes.
Lancet. 2 (8558): 537-40. -
Whiteland, J.L. et al. (1995) Immunohistochemical detection of T-cell subsets and other leukocytes in paraffin-embedded rat and mouse tissues with monoclonal antibodies.
J Histochem Cytochem. 43 (3): 313-20. -
Schwartzkopff, J. et al. (2010) NK cell depletion delays corneal allograft rejection in baby rats.
Mol Vis. 16: 1928-35. -
Fujiki, M. et al. (2010) Induced tolerance to rat liver allografts involves the apoptosis of intragraft T cells and the generation of CD4(+)CD25(+)FoxP3(+) T regulatory cells.
Liver Transpl. 16: 147-54.
View The Latest Product References
-
Ghiringhelli, F. (2005) Tumor cells convert immature myeloid dendritic cells into TGF-beta-secreting cells inducing CD4+CD25+ regulatory T cell proliferation.
J Exp Med. 202: 919-29. -
Aricha, R. et al. (2016) Suppression of experimental autoimmune myasthenia gravis by autologous T regulatory cells.
J Autoimmun. 67: 57-64. -
Lühder, F. et al. (2017) Laquinimod enhances central nervous system barrier functions.
Neurobiol Dis. 102: 60-9. -
Sun, J. et al. (2017) Pentapeptide PLNPK ameliorates adjuvant arthritis and inhibits T cell activation by suppressing Lck and PI3K activities
Int J Clin Exp Pathol. 10(5): 5252-62. -
Timrott, K. et al. (2020) The importance of MHC class II in allogeneic bone marrow transplantation and chimerism-based solid organ tolerance in a rat model.
PLoS One. 15 (5): e0233497. -
Koppe, C. et al. (2021) Local Inflammatory Response after Intramuscularly Implantation of Anti-Adhesive Plasma-Fluorocarbon-Polymer Coated Ti6AI4V Discs in Rats.
Polymers (Basel). 13 (16): 2684. -
Schmiedl, A. et al. (2021) Lung development and immune status under chronic LPS exposure in rat pups with and without CD26/DPP4 deficiency.
Cell Tissue Res. 386 (3): 617-36. -
Zakerkish, F. et al. (2021) Differential effects of the immunosuppressive calcineurin inhibitors cyclosporine-A and tacrolimus on ovulation in a murine model.
Hum Reprod Open. 2021 (2): hoab012. -
Zhou, X. et al. (2022) Dusp6 deficiency attenuates neutrophil-mediated cardiac damage in the acute inflammatory phase of myocardial infarction.
Nat Commun. 13 (1): 6672. -
Hoene, A. et al. (2020) Effects of copper-impregnated collagen implants on local pro- and anti-inflammatory and regenerative tissue reactions following implantation in rats.
J Biomed Mater Res A. 108 (4): 871-81.
- Synonyms
- IL-2R Alpha Chain
- RRID
- AB_324849
- UniProt
- P26897
- Entrez Gene
- Il2ra
- GO Terms
- GO:0002437 inflammatory response to antigenic stimulus
- GO:0016021 integral to membrane
- GO:0004911 interleukin-2 receptor activity
- GO:0008144 drug binding
- GO:0045582 positive regulation of T cell differentiation
- GO:0050687 negative regulation of defense response to virus
- GO:0050777 negative regulation of immune response
MCA273A647
150668If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
View all Anti-Rat ProductsAlways be the first to know.
When we launch new products and resources to help you achieve more in the lab.
Yes, sign me up