ABCA1 antibody | 5A1-1422
Rat anti Mouse ABCA1:Alexa Fluor® 647
- Product Type
- Monoclonal Antibody
- Clone
- 5A1-1422
- Isotype
- IgG2a
- Specificity
- ABCA1
Antibody Quality and Performance Guarantee
| Rat anti Mouse ABCA1 antibody, clone 5A1.1422 recognizes murine adenosine triphosphate (ATP) Binding cassette transporter 1 (ABCA1). The ABC transporters are a large family of conserved proteins that transport a wide variety of molecules across cellular membranes. ABCA1 is a member of the ABC-A sub-family, which acts as a lipid translocator. The molecule was originally identified as a scavenger receptor on macrophages and research shows that ABCA1 also plays a major role in cholesterol metabolism. ABCA1 may play an important role in protecting against cardiovascular disease. Mutations in ABCA1 gene have been associated with Tangier disease, a genetic disorder of lipid metabolism, and the more common Familial HDL deficiency, peresenting with many of the same symproms. |
- Target Species
- Mouse
- Product Form
- Purified IgG conjugated to Alexa Fluor® 647- liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin - Immunogen
- ABCA1 transfected HeLa cells.
- Approx. Protein Concentrations
- IgG concentration 0.05mg/ml
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) Alexa Fluor®647 650 665 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
Neat | 1/10 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Rat IgG2a Negative Control:Alexa Fluor® 647 | MCA1212A647 | F | 100 Tests/1ml |
|
Log in | ||
| List Price | Your Price | ||||||
|
|
Log in | ||||||
| Description | Rat IgG2a Negative Control:Alexa Fluor® 647 | ||||||
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse Seroblock FcR | BUF041A | F | 0.1 mg |
|
Log in | ||
| List Price | Your Price | ||||||
|
|
Log in | ||||||
| Description | Mouse Seroblock FcR | ||||||
| Mouse Seroblock FcR | BUF041B | F | 0.5 mg |
|
Log in | ||
| List Price | Your Price | ||||||
|
|
Log in | ||||||
| Description | Mouse Seroblock FcR | ||||||
References for ABCA1 antibody
-
Pradel, L.C. et al. (2009) ATP-binding cassette transporter hallmarks tissue macrophages and modulates cytokine-triggered polarization programs.
Eur J Immunol. 39 (8): 2270-80. -
Wang, Y. et al. (2019) Smooth Muscle Cells Contribute the Majority of Foam Cells in ApoE (Apolipoprotein E)-Deficient Mouse Atherosclerosis.
Arterioscler Thromb Vasc Biol. 39 (5): 876-87.
Further Reading
-
Zarubica, A. et al. (2007) ABCA1, from pathology to membrane function.
Pflugers Arch. 453 (5): 569-79.
- RRID
- AB_1604582
- UniProt
- P41233
- Entrez Gene
- Abca1
- GO Terms
- GO:0002790 peptide secretion
- GO:0005887 integral to plasma membrane
- GO:0005524 ATP binding
- GO:0005548 phospholipid transporter activity
- GO:0005794 Golgi apparatus
- GO:0006497 protein lipidation
- GO:0006911 phagocytosis, engulfment
- GO:0008203 cholesterol metabolic process
- GO:0008509 anion transmembrane transporter activity
- View More GO Terms
- GO:0016887 ATPase activity
- GO:0010875 positive regulation of cholesterol efflux
- GO:0017127 cholesterol transporter activity
- GO:0033344 cholesterol efflux
- GO:0033700 phospholipid efflux
- GO:0043691 reverse cholesterol transport
- GO:0045332 phospholipid translocation
- GO:0071222 cellular response to lipopolysaccharide
- GO:0071300 cellular response to retinoic acid
MCA2681A647
153267
164085
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
Request a different product with this specificity
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
View all Anti-Mouse ProductsAlways be the first to know.
When we launch new products and resources to help you achieve more in the lab.
Yes, sign me up