Granulocytes antibody | 2B2
Mouse anti Pig Granulocytes:Alexa Fluor® 647
- Product Type
- Monoclonal Antibody
- Clone
- 2B2
- Isotype
- IgG1
- Specificity
- Granulocytes
Mouse anti Pig granulocytes antibody, clone 2B2 recognizes porcine basophils, neutrophils and eosinophils, acting as a reliable tool for their analysis and isolation, without contamination from other cells. Analysis of porcine bone marrow and blood shows that the antigen recognized by clone 2B2 is selectively expressed on mature cells and that it is likely to be a labile conformational epitope. Use of clone 2B2 in conjunction with clone 6D10 product code (MCA2599GA), allows for the discrimination and characterization of different porcine granulocyte lineages and also their developmental stages: 6D10-2B2- early myeloid precursors, 6D10+2B2- immature neutrophils, 6D10+2B2+mature neutrophils and 6D10-2B2+ mature eosinophils and basophils. Clone 2B2 has been shown as suitable for use on cytospins (Pérez et al. 2007). |
- Target Species
- Pig
- Product Form
- Purified IgG conjugated to Alexa Fluor 647 - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin - Immunogen
- Porcine bone marrow hematopoietic cells (BMHC)
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Fusion Partners
- Spleen cells from immunized Balb/c mouse were fused with cells of the Sp2/0 mouse myeloma cell line
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) Alexa Fluor®647 650 665 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | Neat |
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
---|---|---|---|---|---|---|---|
Mouse IgG1 Negative Control:Alexa Fluor® 647 | MCA928A647 | F | 100 Tests/1ml | Log in | |||
List Price | Your Price | ||||||
Log in | |||||||
Description | Mouse IgG1 Negative Control:Alexa Fluor® 647 |
Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
---|---|---|---|---|---|---|---|
Mouse anti Pig CD172a:FITC | MCA2312F | F | 0.1 mg | Log in | |||
List Price | Your Price | ||||||
Log in | |||||||
Description | Mouse anti Pig CD172a:FITC |
References for Granulocytes antibody
-
Nguyen, D.N. et al. (2016) Delayed development of systemic immunity in preterm pigs as a model for preterm infants.
Sci Rep. 6: 36816. -
LeLuduec, J.B. et al. (2016) Intradermal vaccination with un-adjuvanted sub-unit vaccines triggers skin innate immunity and confers protective respiratory immunity in domestic swine.
Vaccine. 34 (7): 914-22. -
Andersen, A.D. et al. (2019) Synbiotics Combined with Glutamine Stimulate Brain Development and the Immune System in Preterm Pigs.
J Nutr. 149 (1): 36-45.
Further Reading
-
Pérez, C. et al. (2007) Phenotypic and functional characterization of porcine granulocyte developmental stages using two new markers.
Dev Comp Immunol. 31 (3): 296-306. -
Piriou-guzylack, L. & Salmon, H. (2008) Membrane markers of the immune cells in swine: an update.
Vet Res. 39 (6): 54.
MCA2600A647
150159 167647 1802If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
View all Anti-Pig ProductsAlways be the first to know.
When we launch new products and resources to help you achieve more in the lab.
Yes, sign me up