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CD107b antibody | AC17

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Anti Dog CD107b Antibody, clone AC17 thumbnail image 1 Anti Dog CD107b Antibody, clone AC17 thumbnail image 2
Anti Dog CD107b Antibody, clone AC17 gallery image 1
Peripheral dog granulocytes stained with Mouse anti Dog CD107b:RPE (MCA2558PE) following permeabilisation with Leucoperm® (BUF09).
Anti Dog CD107b Antibody, clone AC17 gallery image 2
Peripheral dog granulocytes stained with Mouse anti Dog CD107b (MCA2558GA) following permeabilization with Leucoperm® (BUF09). Goat anti Mouse IgG (H/L):FITC (STAR117F) used as secondary antibody.

Mouse anti Dog CD107b:RPE

Product Type
Monoclonal Antibody
Clone
AC17
Isotype
IgG1
Specificity
CD107b

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Product Code Applications Pack Size List Price Your Price Qty
MCA2558PE
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F * 100 Tests loader
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Mouse anti Dog CD107b antibody, clone AC17 recognizes canine CD107b, otherwise known as lysosome-associated membrane protein 2 or LAMP-2. Immunofluorescence staining of MDCK cells with mouse anti dog CD107b, clone AC17 demonstrates staining patterns consistent with localization to lysozomes. This is supported by coincident staining of an exogenous lysozomal glycoprotein, avian LEP100 transfected into MDCK cells and detected using the anti LEP100 antibody clone CV24 (Nabi et al.1991).

Mouse anti Dog CD107b antibody, clone AC17 immunoprecipitates a protein of ~95 kDa in MDCK cells which, following Endo F digestion to remove N-linked oligosaccharides, yields a core protein product of 40 kDa, indicating the heavily glycosylated nature of CD107b. The molecular weight of canine CD107b is typical of many lysozome-associated membrane proteins. While most (97%) CD107b resides in the lysozomal environment in adherent MDCK cells in vitro, a small percentage is associated with the cell membrane (Nabi et al.1991).

CD107b has been shown to share high N-terminal amino acid sequence homology with human, mouse and rat CD107b (Nabi et al.1993).

Transfection of a mink type II lung epithelial cell line with beta1-6-N-acetylglucosaminyl transferase V demonstrates the formation of large lysozomal vacuoles, termed multilamellar bodies (MLBs), having a very distinct phenotype with expression of CD107b, as indicated by immunofluorescent staining with clone AC17. These MLBs require lysozomal degradation via an autophagic pathway for their formation and may have implications for lysozomal storage diseases (Hariri et al.2000). CD107b is involved in the lysosomal uptake of cytosolic proteins and the endocytic pathway.

Mouse anti Dog CD107b antibody, clone AC17 is suitable for use in electron microscopy (Nabi et al.1991).

Target Species
Dog
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Mink
Human
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution
Reconstitute with 1ml distilled water
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
5%Sucrose
Immunogen
MDCK (Madin-Darby Canine Kidney) cells
Fusion Partners
Spleen cells from immunised Balb/c mice were fused with cells of the NS1 myeloma cell line
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
RPE 488nm laser 496 578
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Prior to reconstitution store at +4oC.
After reconstitution store at +4oC.
DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1 Neat
  1. 1 Membrane permeabilization is required for this application. The use of Leucoperm (Product Code BUF09) is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.

How to Use the Spectraviewer

Watch the Tool Tutorial Video ▸
  • Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
  • Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
  • Select the lasers and filters you wish to include
  • Select combined or multi-laser view to visualize the spectra

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG1 Negative Control:RPE MCA928PE F 100 Tests
>
List Price Your Price
Description Mouse IgG1 Negative Control:RPE

References for CD107b antibody

  1. Nabi, I.R. et al. (1991) An endogenous MDCK lysosomal membrane glycoprotein is targeted basolaterally before delivery to lysosomes.
    J Cell Biol. 115 (6): 1573-84.
  2. Nabi, I.R. & Rodriguez-Boulan, E. (1993) Increased LAMP-2 polylactosamine glycosylation is associated with its slower Golgi transit during establishment of a polarized MDCK epithelial monolayer.
    Mol Biol Cell. 4 (6): 627-35.
  3. Hariri, M. et al. (2000) Biogenesis of multilamellar bodies via autophagy.
    Mol Biol Cell. 11: 255-68.
  4. Jou, T.S. et al. (2000) Selective alterations in biosynthetic and endocytic protein traffic in Madin-Darby canine kidney epithelial cells expressing mutants of the small GTPase Rac1.
    Mol Biol Cell. 11 (1): 287-304.
  5. Ihrke, G. et al. (2001) Competing sorting signals guide endolyn along a novel route to lysosomes in MDCK cells.
    EMBO J. 20 (22): 6256-64.
  6. Cliffe, S.T. et al. (2009) SLC29A3 gene is mutated in pigmented hypertrichosis with insulin-dependent diabetes mellitus syndrome and interacts with the insulin signaling pathway.
    Hum Mol Genet. 18: 2257-65.
  7. Pluhar, G.E. et al. (2010) Anti-tumor immune response correlates with neurological symptoms in a dog with spontaneous astrocytoma treated by gene and vaccine therapy.
    Vaccine 28 (19): 3371-8.
  8. Nagahama, M. et al. (2011) Cellular vacuolation induced by Clostridium perfringens epsilon-toxin.
    FEBS J. 278: 3395-407.
    9. View The Latest Product References
  9. Bai, Y. et al. (2011) Intracellular neutralization of viral infection in polarized epithelial cells by neonatal Fc receptor (FcRn)-mediated IgG transport.
    Proc Natl Acad Sci U S A. 108 (45): 18406-11.
  10. Nagahama, M. et al. (2012) Intracellular trafficking of Clostridium perfringens iota-toxin b.
    Infect Immun. 80: 3410-6.

Further Reading

  1. Fukuda, M. (1991) Lysosomal membrane glycoproteins. Structure, biosynthesis, and intracellular trafficking.
    J Biol Chem. 266 (32): 21327-30.

Flow Cytometry

Immunofluorescence

Synonyms
LAMP-2
RRID
AB_10897185

MCA2558PE

155267

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