CD79a antibody | HM57
Mouse anti Human CD79a:RPE
- Product Type
- Monoclonal Antibody
- Clone
- HM57
- Isotype
- IgG1
- Specificity
- CD79a
Mouse anti Human CD79a antibody, clone HM57 recognizes an epitope within the cytoplasmic domain of CD79a. CD79a, also known as mb-1, is a 45 kDa protein that is expressed by B lymphocytes during differentiation from early pre-B cell stage through to plasma cells. The CD79a molecule associates with CD79b (B29) to form a heterodimer that is non-covalently linked to surface immunoglobulin, forming the B-cell receptor (BCR) complex. The CD79a/CD79b heterodimers are also necessary for intracellular signaling following antigen-binding to surface immunoglobulin. |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Rabbit Horse Pig Monkey Rat Bovine Guinea Pig Fallow deer American Bison Red deer Ferret Goat Dog - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
- Reconstitution
- MCA2538PET: Reconstitute with 0.25ml distilled water
- MCA2538PE: Reconstitute with 1ml distilled water
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide (NaN3) 1% Bovine Serum Albumin 5% Sucrose - Immunogen
- Synthetic peptide corresponding to 202-216 amino acid sequence of human mb-1
- Fusion Partners
- Spleen cells from immunized Balb/c mice were fused with cells of the Sp2/0 myeloma cell line
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) RPE 488nm laser 496 578 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
After reconstitution store at +4oC.
DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry 1 | Neat |
- 1 Membrane permeabilization is required for this application. The use of Leucoperm (Product Code BUF09) is recommended for this purpose.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
---|---|---|---|---|---|---|---|
Mouse IgG1 Negative Control:RPE | MCA928PE | F | 100 Tests | Log in | |||
List Price | Your Price | ||||||
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Description | Mouse IgG1 Negative Control:RPE |
Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
---|---|---|---|---|---|---|---|
Human Seroblock | BUF070A | F | 50 Test | Log in | |||
List Price | Your Price | ||||||
Log in | |||||||
Description | Human Seroblock | ||||||
Human Seroblock | BUF070B | F | 200 Test | Log in | |||
List Price | Your Price | ||||||
Log in | |||||||
Description | Human Seroblock |
Source Reference
-
Mason, D.Y. et al. (1991) The IgM-associated protein mb-1 as a marker of normal and neoplastic B cells.
J Immunol. 147 (11): 2474-82.
References for CD79a antibody
-
Jones, M. et al. (1993) Detection of T and B cells in many animal species using cross-reactive anti-peptide antibodies.
J Immunol. 150 (12): 5429-35. -
Nelson, D.D. et al. (2010) CD8(+)/perforin(+)/WC1(-) gammadelta T cells, not CD8(+) alphabeta T cells, infiltrate vasculitis lesions of American bison (Bison bison) with experimental sheep-associated malignant catarrhal fever.
Vet Immunol Immunopathol. 136: 284-91. -
De Schauwer, C. et al. (2012) In search for cross-reactivity to immunophenotype equine mesenchymal stromal cells by multicolor flow cytometry.
Cytometry A. 81 (4): 312-23. -
Spaas, J.H. et al. (2013) Culture and characterisation of equine peripheral blood mesenchymal stromal cells.
Vet J. 195 (1): 107-13. -
Moore, P.F. et al. (2013) Canine inflamed nonepitheliotropic cutaneous T-cell lymphoma: a diagnostic conundrum.
Vet Dermatol. 24 (1): 204-11.e44-5. -
De Schauwer, C. et al. (2014) Characterization and profiling of immunomodulatory genes of equine mesenchymal stromal cells from non-invasive sources.
Stem Cell Res Ther. 5 (1): 6. -
Bozkurt, Y.A., et al. (2014) Histological and immunohistological studies of the structure of lymph nodes in Kilis goats.
Biotech Histochem. 89(6):440-5. -
Paebst, F. et al. (2014) Comparative immunophenotyping of equine multipotent mesenchymal stromal cells: an approach toward a standardized definition.
Cytometry A. 85 (8): 678-87.
View The Latest Product References
-
Gelain ME et al. (2014) CD44 in canine leukemia: analysis of mRNA and protein expression in peripheral blood.
Vet Immunol Immunopathol. 159 (1-2): 91-6. -
Claessen, C. et al. (2015) Equid herpesvirus 1 (EHV1) infection of equine mesenchymal stem cells induces a pUL56-dependent downregulation of select cell surface markers.
Vet Microbiol. 176 (1-2): 32-9. -
Aresu, L. et al. (2015) Canine indolent and aggressive lymphoma: clinical spectrum with histologic correlation.
Vet Comp Oncol. 13 (4): 348-62. -
Poggi, A. et al. (2015) Flow cytometric evaluation of ki67 for the determination of malignancy grade in canine lymphoma.
Vet Comp Oncol. 13 (4): 475-80. -
Froment, R. & Bédard, C. (2016) Marked hyperphosphatasemia associated with an acute leukemia in a Great Dane.
Vet Clin Pathol. 45 (3): 459-65. -
Schinköthe J >et al. (2016) Characterization of tuberculous granulomas in different stages of progression and associated tertiary lymphoid tissue in goats experimentally infected with Mycobacterium avium subsp. hominissuis.
Comp Immunol Microbiol Infect Dis. 47: 41-51. -
Novacco, M. et al. (2016) Prognostic factors in canine acute leukaemias: a retrospective study.
Vet Comp Oncol. 14 (4): 409-16. -
Hillmann, A. et al. (2016) Comparative Characterization of Human and Equine Mesenchymal Stromal Cells: A Basis for Translational Studies in the Equine Model.
Cell Transplant. 25 (1): 109-24. -
Long, H. et al. (2016) Polyostotic Lymphoma in a Ferret (Mustela putorius furo).
J Comp Pathol. 154 (4): 341-4. -
Wessels, M. et al. (2017) Systemic necrotizing polyarteritis in three weaned lambs from one flock.
J Vet Diagn Invest 29 (5) :733-37. -
Uitterdijk, A. et al. (2017) Time course of VCAM-1 expression in reperfused myocardial infarction in swine and its relation to retention of intracoronary administered bone marrow-derived mononuclear cells.
PLoS One. 12 (6): e0178779. -
Nagata, K. et al. (2017) Epstein-Barr Virus Lytic Reactivation Activates B Cells Polyclonally and Induces Activation-Induced Cytidine Deaminase Expression: A Mechanism Underlying Autoimmunity and Its Contribution to Graves' Disease.
Viral Immunol. 30 (3): 240-9. -
Collins, J.J.P. et al. (2018) Impaired Angiogenic Supportive Capacity and Altered Gene Expression Profile of Resident CD146+ Mesenchymal Stromal Cells Isolated from Hyperoxia-Injured Neonatal Rat Lungs.
Stem Cells Dev. 27 (16): 1109-24. -
Murphy, E.G. et al. (2019) First detection of Hepatitis E virus (Orthohepevirus C) in wild brown rats (Rattus norvegicus.) from Great Britain.
Zoonoses Public Health. 66 (6): 686-94. -
Skovdal, S.M. et al. (2019) Inhaled nebulized glatiramer acetate against Gram-negative bacteria is not associated with adverse pulmonary reactions in healthy, young adult female pigs.
PLoS One. 14 (10): e0223647. -
Mu&mtilde;oz-Silvestre, A. et al. (2020) Pathogenesis of Intradermal Staphylococcal Infections: Rabbit Experimental Approach to Natural Staphylococcus aureus Skin Infections.
Am J Pathol. 190 (6): 1188-210. -
Forner, R. et al. (2021) Distribution difference of colostrum-derived B and T cells subsets in gilts and sows.
PLoS One. 16 (5): e0249366. -
Matsuyama, S. et al. (2021) Properties of macrophages and lymphocytes appearing in rat renal fibrosis followed by repeated injection of cisplatin.
J Vet Med Sci. 83 (9): 1435-42. -
Carroll, C.S.E. et al. (2021) Simple and effective bacterial-based intratumoral cancer immunotherapy.
J Immunother Cancer.9(9):e002688. -
Maciag, S.S. et al. (2022) The influence of source of porcine colostrum in development of early immune ontogeny in the piglet
Res Sq. Mar 24 [Epub ahead of print]. -
do Prado Duzanski, A. et al. (2022) Cell-mediated immunity and expression of MHC class I and class II molecules in dogs naturally infected by canine transmissible venereal tumor: Is there complete spontaneous regression outside the experimental CTVT?
Res Vet Sci. 145: 193-204. -
Korbonits, L. et al. (2022) Mycobacterium avium subsp. paratuberculosis Infected Cows Reveal Divergent Immune Response in Bovine Peripheral Blood Derived Lymphocyte Proteome.
Metabolites. 12 (10): 924. -
Schilloks, M.C. et al. (2023) Effects of GHR Deficiency and Juvenile Hypoglycemia on Immune Cells of a Porcine Model for Laron Syndrome.
Biomolecules. 13 (4): 597. -
dos Santos, M.C. et al. (2023) Effect of yeast extracted β-glucans on the immune response and reproductive performance of gilts in the adaptation, gestation, and lactation periods
Livestock Science. 275: 105289. -
Haach, V. et al. (2023) A polyvalent virosomal influenza vaccine induces broad cellular and humoral immunity in pigs.
Virol J. 20 (1): 181. -
Viitanen, S.J. et al. (2023) Escherichia coli-associated follicular cystitis in dogs: Clinical and pathologic characterization.
J Vet Intern Med. 37 (3): 1059-66. -
Martini, V. et al. (2018) A retrospective study of flow cytometric characterization of suspected extranodal lymphomas in dogs.
J Vet Diagn Invest. 30 (6): 830-6. -
Cha, S. et al. (2023) Non-B, Non-T Acute Lymphoblastic Leukemia in a Cat
Journal of Veterinary Clinics. 40 (4): 298-302. -
Giese, I.M. et al. (2020) Chronic Hyperglycemia Drives Functional Impairment of Lymphocytes in Diabetic INS(C94Y) Transgenic Pigs.
Front Immunol. 11: 607473. -
Keller, A.K. et al. (2019) POST-TRAUMATIC OCULAR LYMPHOMA IN THREE RABBITS (ORYCTOLAGUS CUNICULUS)
Journal of Exotic Pet Medicine. 28: 154-61.
Further Reading
-
Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.
Vet Res. 39: 54.
- Synonyms
- MB-1
- RRID
- AB_931716
- UniProt
- P11912
- Entrez Gene
- CD79A
- GO Terms
- GO:0016021 integral to membrane
- GO:0004888 transmembrane receptor activity
- GO:0005771 multivesicular body
- GO:0009897 external side of plasma membrane
- GO:0019815 B cell receptor complex
- GO:0030183 B cell differentiation
- GO:0042100 B cell proliferation
- GO:0045121 membrane raft
- GO:0050853 B cell receptor signaling pathway
- View More GO Terms
MCA2538PET
INN1609MCA2538PE
153591 INN1609If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
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