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CD74 antibody | Bu45

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Anti Human CD74 Antibody, clone Bu45 thumbnail image 1
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Figure A. RPE-Alexa Fluor 750 tandem conjugated Mouse anti Human CD19 (MCA1940P750) and FITC conjugated Mouse IgG1 isotype control (MCA928F). Figure B. RPE-Alexa Fluor 750 tandem conjugated Mouse anti Human CD19 (MCA1940P750) and FITC conjugated Mouse anti Human CD74 (MCA2502F). All experiments performed on human Peripheral blood lymphocytes in the presence of human SeroBlock (BUF070A).

Mouse anti Human CD74:FITC

Product Type
Monoclonal Antibody
Clone
Bu45
Isotype
IgG1
Specificity
CD74

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Product Code Applications Pack Size List Price Your Price Qty
MCA2502F
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F * 0.1 mg loader
List Price Your Price
loader

Mouse anti Human CD74 antibody, clone Bu45 recognizes human CD74, a type II transmembrane protein, also known as the MHC class II associated invariant chain. The Bu45 antibody recognizes 33, 35 and 41 kDa isoforms of CD74. The CD74 molecule plays a critical role in the presentation of peptides, by the MHC class II antigens, to CD4 positive lymphocytes.

CD74 is expressed on MHC class II positive cells including B cells, a subset of activated T cells, monocytes, dendritic cells and by various types of carcinoma. Expression of CD74 is primarily intracellular with moderate expression at the cell surface of B cells and monocytes. Mouse anti Human CD74 antibody, clone Bu45 binds to an epitope within the extracytoplasmic domain of the CD74 molecule, which is distal from the C-terminus.

Target Species
Human
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
1%Bovine Serum Albumin
Immunogen
B lymphoblastoid cell line HFB1.
Approx. Protein Concentrations
IgG concentration 0.1mg/ml
Fusion Partners
Spleen cells from immunized mice were fused with cells of the Ag8.653 myeloma cell line.
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
FITC 490 525
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1 Neat 1/5
  1. 1 Results maybe enhanced using membrane permeabilisation. Membrane permeabilization is required for this application. The use of Leucoperm (Product Code BUF09) is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG1 Negative Control:FITC MCA928F F 100 Tests loader
>
List Price Your Price
loader
Description Mouse IgG1 Negative Control:FITC

Description Product Code Applications Pack Size List Price Your Price Quantity
Human Seroblock BUF070A F 50 Test
List Price Your Price
Description Human Seroblock
Human Seroblock BUF070B F 200 Test
List Price Your Price
Description Human Seroblock
Leucoperm BUF09 F 50 Tests
List Price Your Price
Description Leucoperm

References for CD74 antibody

  1. Wraight, C.J. et al. (1990) Human major histocompatibility complex class II invariant chain is expressed on the cell surface.
    J Biol Chem. 265 (10): 5787-92.
  2. van Bergen, J. et al. (1997) Efficient loading of HLA-DR with a T helper epitope by genetic exchange of CLIP.
    Proc Natl Acad Sci U S A. 94: 7499-502.
  3. Beswick, E.J. et al. (2005) Helicobacter pylori binds to CD74 on gastric epithelial cells and stimulates interleukin-8 production.
    Infect Immun. 73: 2736-43.
  4. Barrera, C.A. et al. (2005) Polarized expression of CD74 by gastric epithelial cells.
    J Histochem Cytochem. 53: 1481-9.
  5. Beswick, E.J. et al. (2006) The Helicobacter pylori urease B subunit binds to CD74 on gastric epithelial cells and induces NF-kappaB activation and interleukin-8 production.
    Infect Immun. 74: 1148-55.
  6. Lapaque, N. et al. (2009) The HLA-DRalpha chain is modified by polyubiquitination.
    J Biol Chem. 284: 7007-16.
  7. Goldenberg, D.M. et al. (2019) Subcutaneous administration of anti-CD74 antibody for systemic lupus erythematosus
    US patent US 20170266281 A1

Flow Cytometry

Functional Assays

Immunofluorescence

Immunoprecipitation

RRID
AB_844513
UniProt
P04233
Entrez Gene
CD74

MCA2502F

159573

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