CD28 antibody | E18
Mouse anti Mouse CD28:Low Endotoxin
- Product Type
- Monoclonal Antibody
- Clone
- E18
- Isotype
- IgG2b
- Specificity
- CD28
Mouse anti Mouse CD28 antibody, clone E18 recognizes murine CD28, a co-stimulatory cell surface molecule expressed by T cell subsets. Clone E18 has been shown to be able to stimulate murine T lymphocytes in combination with anti-mouse CD3. |
- Target Species
- Mouse
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present.
- Immunogen
- Alternate immunization with A20 cells expressing mCD28 and recombinant mCD28Ig.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Endotoxin Level
- < 0.01 EU/ug
- Fusion Partners
- Spleen cells from immunized CD28-/-knockout mice were fused with cells of the X63Ag8.653 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | 1/50 | 1/100 | |
Functional Assays |
- Flow Cytometry
- Use10ul of the suggested working dilution to label 106 cells in 100ul.
References for CD28 antibody
-
Dennehy, K.M. et al. (2006) Cutting edge: monovalency of CD28 maintains the antigen dependence of T cell costimulatory responses.
J Immunol. 176 (10): 5725-9. -
Beyersdorf, N. et al. (2008) Protection from graft-versus-host disease with a novel B7 binding site-specific mouse anti-mouse CD28 monoclonal antibody.
Blood. 112 (10): 4328-36. -
Gogishvili, T. et al. (2012) Interruption of CD28-mediated costimulation during allergen challenge protects mice from allergic airway disease.
J Allergy Clin Immunol. 130 (6): 1394-403.e4. -
Pletinckx, K. et al. (2015) Immature dendritic cells convert anergic nonregulatory T cells into Foxp3- IL-10+ regulatory T cells by engaging CD28 and CTLA-4.
Eur J Immunol. 45 (2): 480-91.
- RRID
- AB_618658
- UniProt
- P31041
- Entrez Gene
- Cd28
- GO Terms
- GO:0002863 positive regulation of inflammatory response to antigenic stimulus
- GO:0016021 integral to membrane
- GO:0006955 immune response
- GO:0008624 induction of apoptosis by extracellular signals
- GO:0009897 external side of plasma membrane
- GO:0031295 T cell costimulation
- GO:0045060 negative thymic T cell selection
- GO:0045086 positive regulation of interleukin-2 biosynthetic process
- GO:0045840 positive regulation of mitosis
- View More GO Terms
- GO:0045944 positive regulation of transcription from RNA polymerase II promoter
- GO:0046641 positive regulation of alpha-beta T cell proliferation
- GO:0048304 positive regulation of isotype switching to IgG isotypes
MCA2473EL
148509 160252 164678If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
View all Anti-Mouse ProductsAlways be the first to know.
When we launch new products and resources to help you achieve more in the lab.
Yes, sign me up