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CD279 antibody | RMP1-30

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Rat anti Mouse CD279 antibody, clone RMP1-30 (MCA2464) used to detect PD-1 (CD279) expression in mouse cells by flow cytometry.
Image caption:
Confirmation of PD-1 expression as a marker of CD8+CD122+ regulatory T-cells in the CNS of PLPmut mice. (A) Representative example of flow cytometric analysis of T-lymphocytes freshly isolated from brains of untreated PLPmut mice gated for CD8 and CD122 expression. The majority of the CNS-derived CD8+ T-cells are CD122- effector cells (red); only few cells are double-positive for CD8 and CD122 (green) and thus regulatory T-cells. (B) Representative example of flow cytometric analysis of gated CD8+CD122- (red) and CD8+CD122+ (green) T-lymphocytes from brains of untreated PLPmut mice analysed for PD-1-coexpression. Only CD8+CD122+ regulatory cells coexpress PD-1. Right histograms display corresponding counts for CD8+CD122- (red) and CD8+CD122+ cells (green) and their coexpression of PD-1. Appropriate isotype control counts are represented in grey

From: Groh J, Hörner M, Martini R.
Teriflunomide attenuates neuroinflammation-related neural damage in mice carrying human PLP1 mutations.
J Neuroinflammation. 2018 Jul 3;15(1):194.
doi: 10.1186/s12974-018-1228-z.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.

Rat anti Mouse CD279:Alexa Fluor® 488

Product Type
Monoclonal Antibody
Clone
RMP1-30
Isotype
IgG2b
Specificity
CD279

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Product Code Applications Pack Size List Price Your Price Qty
MCA2464A488
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F 100 Tests/1ml loader
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Rat anti Mouse CD279 antibody, clone RMP1-30 recognizes mouse CD279, a ~55 kDa cell surface protein, a member of the CD28/CTLA-4 family, otherwise known as Programmed Death-1 (PD-1). CD279 is expressed predominantly on activated T- and B- lymphocytes and on a subset of thymocytes.

CD279, an immunoinhibitory receptor, plays a critical role in peripheral tolerance induction and prevention of autoimmune disease (Ansari et al. 2003). Two members of the B7 family, B7-H1 (PD-L1) and B7-DC (PD-L2), have been identified as the ligands for CD279.

Rat anti Mouse CD279 antibody, clone RMP1-30 does not block the binding of either B7-H1-Ig or B7-DC-Ig fusion proteins to PD-1 transfected BHK cells.

Target Species
Mouse
Product Form
Purified IgG conjugated to Alexa Fluor®488-liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Immunogen
PD-1 transferred BHK cells.
Approx. Protein Concentrations
IgG concentration 0.05 mg/ml
Fusion Partners
Spleen cells from immunised Sprague Dawley rats were fused with cells of the P3U1 myeloma cell line.
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
Alexa Fluor®488 495 519
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

References for CD279 antibody

  1. Matsumoto, K. et al. (2004) B7-DC regulates asthmatic response by an IFN-gamma-dependent mechanism.
    J Immunol. 172 (4): 2530-41.
  2. Bartkowiak, T. (2013) Novel Imaging-Based Techniques Reveal a Role for PD-1/PD-L1 in Tumor Immune Surveillance in the Lung.
    UT GSBS Dissertations and Theses (Open Access). Paper 354.
  3. Hu, Z. et al. (2013) Regulatory CD8+ T cells associated with erosion of immune surveillance in persistent virus infection suppress in vitro and have a reversible proliferative defect.
    J Immunol. 191 (1): 312-22.
  4. Groh, J. et al. (2018) Teriflunomide attenuates neuroinflammation-related neural damage in mice carrying human PLP1 mutations.
    J Neuroinflammation. 15 (1): 194.

Flow Cytometry

Synonyms
PD-1
RRID
AB_961511
UniProt
Q02242
Entrez Gene
Pdcd1
GO Terms
GO:0005515 protein binding
GO:0006915 apoptosis
GO:0016021 integral to membrane
GO:0009897 external side of plasma membrane

MCA2464A488

157278 158128

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