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CD88 antibody | 10/92

Rat anti Mouse CD88:Alexa Fluor® 647

Product Type
Monoclonal Antibody
Clone
10/92
Isotype
IgG2a
Specificity
CD88

Product Code Applications Pack Size List Price Your Price Qty
MCA2456A647T
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 25 Tests/0.25ml loader
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Rat anti Mouse CD88 antibody, clone 10/92 recognizes murine CD88, a 45 kDa G-protein coupled cell surface receptor, otherwise known as C5aR. The CD88 molecule functions as a receptor for the complement component C5a, a potent proinflammatory molecule and chemoattractant for neutrophils to sites of infection. In mouse, CD88 is expressed on granulocytes, monocytes and macrophages but not on resting or stimulated lymphocytes.

Rat anti Mouse CD88 antibody, clone 10/92 does not block the binding of the C5a to murine CD88 (Souri et al. 2003).

Target Species
Mouse
Product Form
Purified IgG conjugated to Alexa Fluor® 647 - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Immunogen
RBL-2H3 transfected cells expressing murine C5aR.
Approx. Protein Concentrations
IgG concentration 0.05 mg/ml
Fusion Partners
Cells from immunised Lou/c rats were fused with cells of the X63-Ag8.653 myeloma cell line.
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
Alexa Fluor®647 650 665
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/5
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.

The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/B/C).

Description Product Code Applications Pack Size List Price Your Price Quantity
Rat IgG2a Negative Control:Alexa Fluor® 647 MCA1212A647 F 100 Tests/1ml loader
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List Price Your Price
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Description Rat IgG2a Negative Control:Alexa Fluor® 647

References for CD88 antibody

  1. Soruri, A. et al. (2003) Characterization of C5aR expression on murine myeloid and lymphoid cells by the use of a novel monoclonal antibody.
    Immunol Lett. 88:47-52.
  2. Ager, R.R. et al. (2010) Microglial C5aR (CD88) expression correlates with amyloid-beta deposition in murine models of Alzheimer's disease.
    J Neurochem. 113: 389-401
  3. Shagdarsuren, E. et al. (2010) C5a Receptor Targeting in Neointima Formation After Arterial Injury in Atherosclerosis-Prone Mice.
    Circulation. 122: 1026-36.
  4. Cudaback, E. et al. (2011) Apolipoprotein E isoform-dependent microglia migration.
    FASEB J. 25: 2082-91.
  5. Manthey, H.D. et al. (2011) Complement C5a inhibition reduces atherosclerosis in ApoE-/- mice.
    FASEB J. 25: 2447-55.
  6. Pavlovski, D. et al. (2012) Generation of complement component C5a by ischemic neurons promotes neuronal apoptosis.
    FASEB J. 26 (9): 3680-90.
  7. Denny, K.J. et al. (2013) C5a receptor signaling prevents folate deficiency-induced neural tube defects in mice.
    J Immunol. 190 (7): 3493-9.
  8. Li, R. et al. (2013) C5L2: a controversial receptor of complement anaphylatoxin, C5a.
    FASEB J. 27 (3): 855-64.
  9. View The Latest Product References
  10. Benson, M.J. et al. (2015) A novel anticonvulsant mechanism via inhibition of complement receptor C5ar1 in murine epilepsy models.
    Neurobiol Dis. 76: 87-97.
  11. Brennan, F.H. et al. (2015) The Complement Receptor C5aR Controls Acute Inflammation and Astrogliosis following Spinal Cord Injury.
    J Neurosci. 35 (16): 6517-31.
  12. Hernandez, M.X. et al. (2017) C5a Increases the Injury to Primary Neurons Elicited by Fibrillar Amyloid Beta.
    ASN Neuro. 9 (1): 1759091416687871.
  13. Benson, M.J. et al. (2017) The effects of C5aR1 on leukocyte infiltration following pilocarpine-induced status epilepticus.
    Epilepsia. 58 (4): e54-e58.

Immunofluorescence

Synonyms
C5aR
RRID
AB_1102426
UniProt
P30993
Entrez Gene
C5ar1
GO Terms
GO:0005886 plasma membrane
GO:0016021 integral to membrane
GO:0004944 C5a anaphylatoxin receptor activity
GO:0050830 defense response to Gram-positive bacterium
GO:0010575 positive regulation vascular endothelial growth factor production
GO:0010759 positive regulation of macrophage chemotaxis
GO:0030593 neutrophil chemotaxis
GO:0032494 response to peptidoglycan
GO:0045766 positive regulation of angiogenesis
GO:0090023 positive regulation of neutrophil chemotaxis

MCA2456A647T

158109 1610

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