CD244 antibody | 2B4.69
Mouse anti Human CD244:Alexa Fluor® 647
- Product Type
- Monoclonal Antibody
- Clone
- 2B4.69
- Isotype
- IgG1
- Specificity
- CD244
Antibody Quality and Performance Guarantee
| Mouse anti Human CD244 antibody, clone 2B4.69 recognizes the CD244 cell surface antigen, also known as natural killer receptor 2B4 (NKG2B4). CD244 is a 370 amino acid single pass type I transmembrane glycoprotein with two Ig-like V type domains. CD244 is a member of the SLAM family of receptors expressed on natural killer (NK) cells, gamma delta T cells, CD14+ monocytes, and a subset of CD8+ T cells. CD244 was originally identified as an activating receptor on NK cells, The receptor may have a wide range of activities including NK cell activation, co-stimulation, or even inhibition, depending on the cell type and stage of differentiation. The ligand of CD244 is CD48, a GPI-anchored membrane protein also belonging to the SLAM family. |
- Target Species
- Human
- Product Form
- Purified IgG conjugated to Alexa Fluor® 647 - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Immunogen
- CD244 Fusion protein.
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Fusion Partners
- Spleen cells from immunized Balb/c mice were fused with cells of the NS-1 myeloma cell line.
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) Alexa Fluor®647 650 665 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
Neat |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control:Alexa Fluor® 647 | MCA928A647 | F | 100 Tests/1ml |
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Log in | ||
| List Price | Your Price | ||||||
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| Description | Mouse IgG1 Negative Control:Alexa Fluor® 647 | ||||||
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Human Seroblock | BUF070A | F | 50 Test | Log in | |||
| List Price | Your Price | ||||||
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| Description | Human Seroblock | ||||||
| Human Seroblock | BUF070B | F | 200 Test | Log in | |||
| List Price | Your Price | ||||||
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| Description | Human Seroblock | ||||||
References for CD244 antibody
-
Romero, X. et al. (2005) CD229 (Ly9) lymphocyte cell surface receptor interacts homophilically through its N-terminal domain and relocalizes to the immunological synapse.
J Immunol. 174 (11): 7033-42. -
Bengsch, B. et al. (2014) Restoration of HBV-specific CD8+ T cell function by PD-1 blockade in inactive carrier patients is linked to T cell differentiation.
J Hepatol. 61 (6): 1212-9. -
Raziorrouh, B. et al. (2010) The Immunoregulatory Role of CD244 in Chronic Hepatitis B Infection and its Inhibitory Potential on Virus-Specific CD81 T-cell Function.
Hepatology. 52: 1934-47. -
Boussaad, I. et al. (2011) Wild-type measles virus interferes with short-term engraftment of human CD34+ hematopoietic progenitor cells.
J Virol. 85: 7710-8.
- UniProt
- Q9BZW8
- Entrez Gene
- CD244
- GO Terms
- GO:0005515 protein binding
- GO:0007596 blood coagulation
- GO:0016021 integral to membrane
- GO:0004872 receptor activity
- GO:0050900 leukocyte migration
MCA2421A647
1611
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