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CD62P antibody | Psel.KO.2.7

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Anti Human CD62P Antibody, clone Psel.KO.2.7 thumbnail image 1 Anti Human CD62P Antibody, clone Psel.KO.2.7 thumbnail image 2 Anti Human CD62P Antibody, clone Psel.KO.2.7 thumbnail image 3 Anti Human CD62P Antibody, clone Psel.KO.2.7 thumbnail image 4 Anti Human CD62P Antibody, clone Psel.KO.2.7 thumbnail image 5 Anti Human CD62P Antibody, clone Psel.KO.2.7 thumbnail image 6
Anti Human CD62P Antibody, clone Psel.KO.2.7 gallery image 1
Staining of thrombin activated human platelets with Mouse anti Human CD62P (MCA2419).
Anti Human CD62P Antibody, clone Psel.KO.2.7 gallery image 2
Staining of thrombin activated human platelets with Mouse anti Human CD62P:Alexa Fluor® 647 (MCA2419A647).
Anti Human CD62P Antibody, clone Psel.KO.2.7 gallery image 3
Staining of thrombin activated human platelets with Mouse anti Human CD62P:RPE (MCA2419PE).
Anti Human CD62P Antibody, clone Psel.KO.2.7 gallery image 4
Staining of thrombin activated dog platelets with Mouse anti Human CD62P (MCA2419) followed by goat anti mouse IgG:FITC (STAR117F).
Anti Human CD62P Antibody, clone Psel.KO.2.7 gallery image 5
Published customer image:
Mouse anti human CD62P, clone Psel.KO.2.7 (MCA2419) used for identification of p-selectin expression in bovine luteal endothelial cells using immunofluorescence.
Image caption:
Effect of PGF2α on P-selectin in LECs. The levels of P-selectin expression after PGF2α (10−6 M) treatment in LECs. Fig. 5A shows P-selectin expression in LECs in control and Fig. 5B shows P-selectin expression in the PGF2α treatment group at 5 min (n = 4 in each group). Scale bars indicate 5 μm. Fig. 5C shows the intensity of P-selectin expression at 5, 15 and 30 after treatment, respectively (% of control). All values are the means ± SEM. Different superscript indicate significant differences (P<0.05) as determined by Student's t-test.

From: Shirasuna K, Jiemtaweeboon S, Raddatz S, Nitta A, Schuberth H-J, Bollwein H, et al. (2012) Rapid Accumulation of Polymorphonuclear Neutrophils in the Corpus luteum during Prostaglandin F2α-Induced Luteolysis in the Cow.
PLoS ONE 7(1): e29054.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Anti Human CD62P Antibody, clone Psel.KO.2.7 gallery image 6
Published customer image:
Mouse anti human CD62P, clone Psel.KO.2.7 (MCA2419) used for evaluation of canine platelet adctivation using flow cytometry.
Image caption:
Flow cytometric analyses of platelet activation by P-selecting labelling. (A) Thrombin activated platelets. (B) Platelet sample after density barrier centrifugation.

From: Trichler SA, Bulla SC, Thomason J, Lunsford KV, Bulla C. Ultra-pure platelet isolation from canine whole blood.
BMC Vet Res. 2013 Jul 17;9:144.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.

Mouse anti Human CD62P:Alexa Fluor® 488

Product Type
Monoclonal Antibody
Clone
Psel.KO.2.7
Isotype
IgG1
Specificity
CD62P

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Product Code Applications Pack Size List Price Your Price Qty
MCA2419A488
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 100 Tests/1ml loader
List Price Your Price
loader

Mouse anti human CD62P, clone Psel.KO.2.7, recognizes human P-Selectin. CD62P is a ~140 kDa surface antigen expressed by activated platelets and endothelial cells, and plays an important role in adhesive processes between leucocytes and endothelial cells.

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Horse
Bovine
Rat
Goat
Cat
Sheep
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Alexa Fluor® 488 - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Immunogen
P-selectin transfected 300.19 cells.
Approx. Protein Concentrations
IgG concentration 0.05 mg/ml
Fusion Partners
Spleen cells from immunized CD62P knock-out mice (strain C57/B6) were fused with cells of the NS-1 myeloma cell line.
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
Alexa Fluor®488 495 519
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/5
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG1 Negative Control:Alexa Fluor® 488 MCA928A488 F 100 Tests/1ml loader
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Description Mouse IgG1 Negative Control:Alexa Fluor® 488

Description Product Code Applications Pack Size List Price Your Price Quantity
Human Seroblock BUF070A F 50 Test
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Description Human Seroblock
Human Seroblock BUF070B F 200 Test
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Description Human Seroblock

Source Reference

  1. Massaguer, A. et al. (2000) Production and characterization of monoclonal antibodies against conserved epitopes of P-selectin (CD62P).
    Tissue Antigens. 56 (2): 117-28.

References for CD62P antibody

  1. Massaguer, A. et al. (2002) Reactivity of CD62P workshop mAbs with resting and activated platelets from different animal species.
    In: Leucocyte Typing VII. Edited by Mason, D. et al. Oxford University Press, pp 342-3.
  2. Johnson, C.A. Jr et al. (2008) Flow cytometric assays for quantifying activated ovine platelets.
    Artif Organs. 32 (2): 136-45.
  3. Johnson, C.A. Jr et al. (2011) Biocompatibility assessment of the first generation PediaFlow pediatric ventricular assist device.
    Artif Organs. 35 (1): 9-21.
  4. Johnson, C.A. Jr et al. (2011) Platelet activation after implantation of the Levitronix PediVAS in the ovine model.
    ASAIO J. 57 (6): 516-21.
  5. Johnson, C.A. Jr. et al. (2011) Platelet activation in ovines undergoing sham surgery or implant of the second generation PediaFlow pediatric ventricular assist device.
    Artif Organs. 35 (6): 602-13.
  6. Shirasuna, K. et al. (2012) Rapid accumulation of polymorphonuclear neutrophils in the Corpus luteum during prostaglandin F(2α)-induced luteolysis in the cow.
    PLoS One. 7: e29054.
  7. Trichler, S.A. et al. (2013) Ultra-pure platelet isolation from canine whole blood.
    BMC Vet Res. 9: 144.
  8. Shankarraman, V. et al. (2014) Biocompatibility Assessment of the CentriMag-Novalung Adult ECMO Circuit in a Model of Acute Pulmonary Hypertension.
    ASAIO J. 60 (4): 429-35.
    9. View The Latest Product References
  9. Iwaszko-Simonik, A. et al. (2015) Expression of surface platelet receptors (CD62P and CD41/61) in horses with recurrent airway obstruction (RAO).
    Vet Immunol Immunopathol. 164 (1-2): 87-92.
  10. Iwaszko-Simonik, A. et al. (2015) Expression of surface platelet receptors (CD62P and CD41/61) in horses with recurrent airway obstruction (RAO).
    Vet Immunol Immunopathol. 164 (1-2): 87-92.
  11. Prat, N.J. et al. (2015) Low-Dose Heparin Anticoagulation During Extracorporeal Life Support for Acute Respiratory Distress Syndrome in Conscious Sheep.
    Shock. 44 (6): 560-8.
  12. Acar, D.D. et al. (2016) Upregulation of endothelial cell adhesion molecules characterizes veins close to granulomatous infiltrates in the renal cortex of cats with feline infectious peritonitis and is indirectly triggered by feline infectious peritonitis virus-infected monocytes in vitro.
    J Gen Virol. 97 (10): 2633-2642.
  13. ChanC, H.H. et al. (2017) Shear Stress-Induced Total Blood Trauma in Multiple Species.
    Artif Organs. 41 (10): 934-947.
  14. Theuerkauf, K. et al. (2022) Activated platelets and platelet-leukocyte aggregates in the equine systemic inflammatory response syndrome.
    J Vet Diagn Invest. 10406387221077969.
  15. Prat, N.J. et al. (2022) Regional blood acidification inhibits coagulation during extracorporeal carbon dioxide removal (ECCO(2) R).
    Artif Organs. 46 (6): 1181-91.

Flow Cytometry

Functional Assays

Synonyms
P-SELECTIN
RRID
AB_566869
UniProt
P16109
P42201
P98106
Entrez Gene
SELP
SELP
Selp
GO Terms
GO:0001530 lipopolysaccharide binding
GO:0002576 platelet degranulation
GO:0005887 integral to plasma membrane
GO:0005615 extracellular space
GO:0005624 membrane fraction
GO:0005625 soluble fraction
GO:0008201 heparin binding
GO:0050829 defense response to Gram-negative bacterium
GO:0009897 external side of plasma membrane
GO:0010572 positive regulation of platelet activation
GO:0030168 platelet activation
GO:0031088 platelet dense granule membrane
GO:0031092 platelet alpha granule membrane
GO:0032496 response to lipopolysaccharide
GO:0033691 sialic acid binding
GO:0042806 fucose binding
GO:0043208 glycosphingolipid binding
GO:0070492 oligosaccharide binding
GO:0007155 cell adhesion
GO:0016021 integral to membrane
GO:0005529 sugar binding
GO:0002691 regulation of cellular extravasation
GO:0006954 inflammatory response
GO:0014070 response to organic cyclic compound
GO:0043499 eukaryotic cell surface binding
GO:0045785 positive regulation of cell adhesion
GO:0048514 blood vessel morphogenesis

MCA2419A488

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