Fc Epsilon R1 Alpha antibody | 9E1
Mouse anti Human Fc Epsilon R1 Alpha:RPE
- Product Type
- Monoclonal Antibody
- Clone
- 9E1
- Isotype
- IgG2b
- Specificity
- Fc Epsilon R1 Alpha
Antibody Quality and Performance Guarantee
| Mouse anti Human Fc epsilon R1 alpha antibody, clone 9E1 recognizes the human high affinity Fc receptor for IgE (Fc epsilon R1), which plays a central role in the IgE-mediated allergic response. Fc epsilon R1 consists of four subunits, a high affinity IgE binding alpha subunit, a beta chain and two di-sulphide linked gamma subunits. Clone 9E1 specifically recognizes the extracellular D1 domain of the Fc epsilon R1 alpha chain. Fc epsilon R1 is primarily expressed on mast cells and basophils but expression of Fc epsilon R1 has also been reported on monocytes, Langerhans cells and dendritic cells from patients with atopic diseases. |
- Target Species
- Human
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
- Reconstitution
- Reconstitute with 1.0 ml distilled water
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
1% bovine serum albumin
5% sucrose - Fusion Partners
- Spleen cells from immunized Balb/c mice were fused with cells of the Sp2/0-Ag14 myeloma cell line.
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) RPE 488nm laser 496 578 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Prior to reconstitution store at +4°C.
After reconstitution store at +4°C.
DO NOT FREEZE.
This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
After reconstitution store at +4°C.
DO NOT FREEZE.
This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
Neat |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells in 100μl.
How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG2b Negative Control:RPE | MCA691PE | F | 100 Tests | Log in | |||
| List Price | Your Price | ||||||
| Log in | |||||||
| Description | Mouse IgG2b Negative Control:RPE | ||||||
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Human Seroblock | BUF070A | F | 50 Test | Log in | |||
| List Price | Your Price | ||||||
| Log in | |||||||
| Description | Human Seroblock | ||||||
| Human Seroblock | BUF070B | F | 200 Test | Log in | |||
| List Price | Your Price | ||||||
| Log in | |||||||
| Description | Human Seroblock | ||||||
References for Fc Epsilon R1 Alpha antibody
-
Vangelista, L. et al. (2002) A minimal receptor-Ig chimera of human FcepsilonRI alpha-chain efficiently binds secretory and membrane IgE.
Protein Eng. 15 (1): 51-7. -
Vangelista, L. et al. (2002) Efficient folding of the FcepsilonRI alpha-chain membrane-proximal domain D2 depends on the presence of the N-terminal domain D1.
J Mol Biol. 322 (4): 815-25. -
Vangelista, L. et al. (2005) Membrane IgE binds and activates Fc epsilon RI in an antigen-independent manner.
J Immunol. 174 (9): 5602-11. -
Gschwandtner, M. et al. (2018) The Reticulum-Associated Protein RTN1A Specifically Identifies Human Dendritic Cells.
J Invest Dermatol. 138 (6): 1318-27.
- RRID
- AB_616857
- UniProt
- P12319
- Entrez Gene
- FCER1A
- GO Terms
- GO:0005887 integral to plasma membrane
MCA2368PE
159084
159816
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
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