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CD321 antibody | H202-106

Rat anti Mouse CD321

Product Type
Monoclonal Antibody
Clone
H202-106
Isotype
IgG1
Specificity
CD321

Product Code Applications Pack Size List Price Your Price Qty
MCA2346
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F IP 0.25 mg loader
List Price Your Price
loader
MCA2346GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F IP 0.1 mg loader
List Price Your Price
loader

Rat anti Mouse CD321 antibody, clone H202-106 recognizes murine CD321, also known as junctional adhesion molecule 1 (JAM-1). CD321 is a 274 amino acid ~32-4 1kDa single pass, type I transmembrane glycoprotein, which shares similarities with related proteins JAM-2 and JAM-3.

CD321 is a multifunctional protein primarily expressed by platelets, endothelial and epithelial cells. The CD321 protein co-localises with tight junction molecules in both epithelial and endothelial cells and plays an important role in the regulation of junctional integrity and permeability. In addition, CD321 is a ligand for the integrin LFA-1 and is also involved in the transmigration of leucocytes.

Target Species
Mouse
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% sodium azide (NaN3)
Carrier Free
Yes
Immunogen
MTE1/MTE2 stromal cell lines.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Cells from immunized Lou rats were fused with cells of the X63 mouse myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/100 1/200
Immunohistology - Frozen
Immunoprecipitation
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10μl of the suggested working dilution to label 106 cells in 100μl

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Description Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed)
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Description Goat anti Rat IgG:DyLight®550 (Mouse Adsorbed)
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Description Goat anti Rat IgG:DyLight®650 (Mouse Adsorbed)
Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) STAR71D800GA F IF WB 0.1 mg loader
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Description Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed)
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Description Goat anti Rat IgG:HRP (Mouse Adsorbed)
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Description Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed)

References for CD321 antibody

  1. Malergue, F. et al. (1998) A novel immunoglobulin superfamily junctional molecule expressed by antigen presenting cells, endothelial cells and platelets.
    Mol Immunol. 35 (17): 1111-9.
  2. Ebnet, K. et al. (2000) Junctional adhesion molecule interacts with the PDZ domain-containing proteins AF-6 and ZO-1.
    J Biol Chem. 275 (36): 27979-88.
  3. Aurrand-lions, M. et al. (2001) Heterogeneity of endothelial junctions is reflected by differential expression and specific subcellular localization of the three JAM family members.
    Blood. 98 (13): 3699-707.
  4. Prestwich, R.J. et al. (2009) Immune-mediated antitumor activity of reovirus is required for therapy and is independent of direct viral oncolysis and replication.
    Clin Cancer Res. 15 (13): 4374-4381.
  5. Morita, Y. et al. (2010) Heterogeneity and hierarchy within the most primitive hematopoietic stem cell compartment.
    J Exp Med. 207 (6): 1173-82.
  6. Schmitt, M.M. et al. (2014) Endothelial junctional adhesion molecule-a guides monocytes into flow-dependent predilection sites of atherosclerosis.
    Circulation. 129 (1): 66-76.
  7. Narni-Mancinelli, E. et al.. (2017) Complement factor P is a ligand for the natural killer cell-activating receptor NKp46.
    Sci Immunol; 2(10): eaam9628.
  8. Nagatake, T. et al. (2020) Selective expression of claudin-5 in thymic endothelial cells regulates the blood-thymus barrier and T-cell export.
    Int Immunol. Oct 10 dxaa069 [Epub ahead of print].
  9. View The Latest Product References

Synonyms
JAM-1
RRID
AB_567266
UniProt
O88792
Entrez Gene
F11r
GO Terms
GO:0007155 cell adhesion
GO:0016021 integral to membrane
GO:0005923 tight junction
GO:0030855 epithelial cell differentiation
GO:0044419 interspecies interaction between organisms

MCA2346

148182 164871

MCA2346GA

150827

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