CD221 antibody | 1H7
Mouse anti Human CD221:Alexa Fluor® 488
- Product Type
- Monoclonal Antibody
- Clone
- 1H7
- Isotype
- IgG1
- Specificity
- CD221
Mouse anti Human CD221 antibody, clone 1H7 recognizes human CD221, a ~155 kDa receptor tyrosine kinase, also known as Insulin-like growth factor I receptor (IGF-I Receptor). CD221 is composed of two extracellular alpha-subunits and two transmembrane beta-subunits. Clone 1H7 recognizes an epitope in the alpha subunits of CD221, demonstrated by western blotting (Li et al.1993). CD221 is expressed in a range of tissues, including kidney, liver, placenta, mammary gland, brain, ovary and skin. The ligands for CD221 include IGF-I and IGF-II, which bind to CD221 and activate tyrosine kinase activity, resulting in phosphorylation of several intracellular signaling proteins. Clone 1H7 is reported to partially block binding of IGF-I and IGF-II to CD221 (Li et al.1993). |
- Target Species
- Human
- Product Form
- Purified IgG conjugated to Alexa Fluor® 488 - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
1% bovine serum albumin - Immunogen
- Purified human placental IGF-I receptor.
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Fusion Partners
- Spleen cells from immunized BALB/c mice were fused with cells of the SP2/0 myeloma cell line.
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) Alexa Fluor®488 495 519 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | Neat | 1/5 |
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells in 100μl
Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
---|---|---|---|---|---|---|---|
Mouse IgG1 Negative Control:Alexa Fluor® 488 | MCA928A488 | F | 100 Tests/1ml | Log in | |||
List Price | Your Price | ||||||
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Description | Mouse IgG1 Negative Control:Alexa Fluor® 488 |
Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
---|---|---|---|---|---|---|---|
Human Seroblock | BUF070A | F | 50 Test | Log in | |||
List Price | Your Price | ||||||
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Description | Human Seroblock | ||||||
Human Seroblock | BUF070B | F | 200 Test | Log in | |||
List Price | Your Price | ||||||
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Description | Human Seroblock |
References for CD221 antibody
-
Li, S.L. et al. (1993) Two new monoclonal antibodies against the alpha subunit of the human insulin-like growth factor-I receptor.
Biochem Biophys Res Commun. 196 (1): 92-8. -
Beauvais, D.M. & Rapraeger, A.C. (2010) Syndecan-1 couples the insulin-like growth factor-1 receptor to inside-out integrin activation.
J Cell Sci. 123 (Pt 21): 3796-807. -
Krieger, C.C. et al. (2016) Thyrotropin/IGF-1 receptor crosstalk in Graves' ophthalmopathy pathogenesis.
J Clin Endocrinol Metab. Apr 4: jc20161315. [Epub ahead of print] -
van Valen, F. et al. (2012) A Novel Role of IGF1 in Apo2L/TRAIL-Mediated Apoptosis of Ewing Tumor Cells.
Sarcoma. 2012: 782970.
- Synonyms
- IGF-1 Receptor
- RRID
- AB_566645
- UniProt
- P08069
- Entrez Gene
- IGF1R
- GO Terms
- GO:0005010 insulin-like growth factor receptor activity
- GO:0005887 integral to plasma membrane
- GO:0005158 insulin receptor binding
- GO:0046872 metal ion binding
- GO:0005524 ATP binding
- GO:0005792 microsome
- GO:0006916 anti-apoptosis
- GO:0006955 immune response
- GO:0008284 positive regulation of cell proliferation
- View More GO Terms
- GO:0008286 insulin receptor signaling pathway
- GO:0042802 identical protein binding
- GO:0014065 phosphatidylinositol 3-kinase cascade
- GO:0048015 phosphatidylinositol-mediated signaling
- GO:0030335 positive regulation of cell migration
- GO:0031994 insulin-like growth factor I binding
- GO:0043548 phosphatidylinositol 3-kinase binding
- GO:0043559 insulin binding
- GO:0043560 insulin receptor substrate binding
- GO:0045740 positive regulation of DNA replication
- GO:0046777 protein autophosphorylation
- GO:0051262 protein tetramerization
MCA2344A488
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