CD312 antibody | 2A1
Mouse anti Human CD312:Alexa Fluor® 647
- Product Type
- Monoclonal Antibody
- Clone
- 2A1
- Isotype
- IgG1
- Specificity
- CD312
Mouse anti Human CD312 antibody, clone 2A1 recognizes human EMR2, a member of the epidermal growth factor-seven transmembrane (EGF-TM7) family of proteins, which is closely related to CD97. EMR2, also known as CD312, is predominantly expressed on myeloid dendritic cells, monocytes and tissue macrophages. Various isoforms of EMR2 have been documented. The ligand for the largest isoform of EMR2 has recently been identified as chrondroitin sulphate, which binds to the fourth EGF-like module of EMR2. Mouse anti Human CD312 antibody, clone 2A1 recognizes the stalk region of EMR2. |
- Target Species
- Human
- Product Form
- Purified IgG conjugated to Alexa Fluor®647- liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Immunogen
- NIH-3T3 cells stably transfected with EMR2 (EGF1-5) cDNA.
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Fusion Partners
- Spleen cells from immunized Balb/c mice were fused with cells of the mouse SP2/0 myeloma cell line.
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) Alexa Fluor®647 650 665 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | Neat |
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
---|---|---|---|---|---|---|---|
Mouse IgG1 Negative Control:Alexa Fluor® 647 | MCA928A647 | F | 100 Tests/1ml | Log in | |||
List Price | Your Price | ||||||
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Description | Mouse IgG1 Negative Control:Alexa Fluor® 647 |
Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
---|---|---|---|---|---|---|---|
Human Seroblock | BUF070A | F | 50 Test | Log in | |||
List Price | Your Price | ||||||
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Description | Human Seroblock | ||||||
Human Seroblock | BUF070B | F | 200 Test | Log in | |||
List Price | Your Price | ||||||
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Description | Human Seroblock |
References for CD312 antibody
-
Kwakkenbos, M.J. et al. (2002) The human EGF-TM7 family member EMR2 is a heterodimeric receptor expressed on myeloid cells.
J Leukoc Biol. 71 (5): 854-62. -
Stacey, M. et al. (2003) The epidermal growth factor-like domains of the human EMR2 receptor mediate cell attachment through chondroitin sulfate glycosaminoglycans.
Blood. 102 (8): 2916-24. -
Lin, H.H. et al. (2004) Autocatalytic cleavage of the EMR2 receptor occurs at a conserved G protein-coupled receptor proteolytic site motif.
J Biol Chem. 279 (30): 31823-32. -
Yona, S. et al. (2008) Ligation of the adhesion-GPCR EMR2 regulates human neutrophil function.
FASEB J. 22 (3): 741-51. -
Tseng, W.Y. et al. (2013) Increased soluble CD4 in serum of rheumatoid arthritis patients is generated by matrix metalloproteinase (MMP)-like proteinases.
PLoS One. 8 (5): e63963. -
Huang, Y.S. et al. (2018) Membrane-association of EMR2/ADGRE2-NTF is regulated by site-specific N-glycosylation.
Sci Rep. 8 (1): 4532. -
Shankar-Hari, M. et al. (2018) Early PREdiction of sepsis using leukocyte surface biomarkers: the ExPRES-sepsis cohort study.
Intensive Care Med. 44 (11): 1836-48. -
Bhudia, N. et al. (2020) G Protein-Coupling of Adhesion GPCRs ADGRE2/EMR2 and ADGRE5/CD97, and Activation of G Protein Signalling by an Anti-EMR2 Antibody.
Sci Rep. 10 (1): 1004.
View The Latest Product References
Further Reading
-
Kwakkenbos, M.J. et al. (2004) The EGF-TM7 family: a postgenomic view.
Immunogenetics. 55 (10): 655-66.
- Synonyms
- EMR2
- RRID
- AB_566726
- UniProt
- Q9UHX3
- Entrez Gene
- EMR2
- GO Terms
- GO:0005886 plasma membrane
- GO:0005509 calcium ion binding
- GO:0016021 integral to membrane
- GO:0004930 G-protein coupled receptor activity
- GO:0007218 neuropeptide signaling pathway
MCA2330A647T
151423MCA2330A647
149099 155975 160670If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
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