Dectin-1 antibody | 2A11
Rat anti Mouse Dectin-1:Alexa Fluor® 647
- Product Type
- Monoclonal Antibody
- Clone
- 2A11
- Isotype
- IgG2b
- Specificity
- Dectin-1
Rat anti Mouse dectin-1 antibody, clone 2A11 recognizes murine beta-glucan receptor, also known as dectin-1 or CD369. Dectin-1 is predominantly expressed by cells of the monocyte/macrophage and neutrophil lineages, but also at lower levels by dendritic cells and a subpopulation of T cells. As a major leucocyte receptor for beta-glucan this molecule may have a key role in the immunomodulatory effects of beta-glucans and in the host response to fungal pathogens. Dectin-1 may stimulate reactive oxygen production in macropahges via the protein tyrosine kinase known as Syk. Rat anti Mouse Dectin-1 antibody, clone 2A11 inhibits the binding of zymosan to macrophages via the beta-glucan receptor. |
- Target Species
- Mouse
- Product Form
- Purified IgG conjugated to Alexa Fluor® 647 - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
1% bovine serum albumin - Immunogen
- Dectin-1 transfected NIH3T3 cells and recombinant soluble Dectin-1.
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Fusion Partners
- Spleen cells from immunized Fischer rats were fused with cells of the rat Y3 myeloma cell line
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) Alexa Fluor®647 650 665 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | Neat | 1/10 |
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells in 100μl
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Mouse Seroblock FcR | BUF041A | F | 0.1 mg | Log in | |||
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Description | Mouse Seroblock FcR | ||||||
Mouse Seroblock FcR | BUF041B | F | 0.5 mg | Log in | |||
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Description | Mouse Seroblock FcR |
Source Reference
-
Brown, G.D. et al. (2002) Dectin-1 is a major beta-glucan receptor on macrophages.
J Exp Med. 196 (3): 407-12.
References for Dectin-1 antibody
-
Taylor, P.R. et al. (2002) The beta-glucan receptor, dectin-1, is predominantly expressed on the surface of cells of the monocyte/macrophage and neutrophil lineages.
J Immunol. 169 (7): 3876-82. -
Reid, D.M. et al. (2004) Expression of the beta-glucan receptor, Dectin-1, on murine leukocytes in situ correlates with its function in pathogen recognition and reveals potential roles in leukocyte interactions.
J Leukoc Biol. 76 (1): 86-94. -
Underhill, D.M. et al. (2005) Dectin-1 activates Syk tyrosine kinase in a dynamic subset of macrophages for reactive oxygen production.
Blood. 106 (7): 2543-50. -
Lefevre,L. et al. (2010) PPARc Ligands Switched High Fat Diet-Induced Macrophage M2b Polarization toward M2a Thereby Improving Intestinal Candida Elimination
PLoS One. 5(9):e12828. -
Fei, M. et al. (2011) TNF-alpha from inflammatory dendritic cells (DCs) regulates lung IL-17A/IL-5 levels and neutrophilia versus eosinophilia during persistent fungal infection.
Proc Natl Acad Sci U S A. 108 (13): 5360-5. -
Gazi, U. et al. (2011) Fungal Recognition Enhances Mannose Receptor Shedding through Dectin-1 Engagement.
J Biol Chem. 286: 7822-9. -
McDonald, J.U. et al. (2011) In vivo functional analysis and genetic modification of in vitro-derived mouse neutrophils.
FASEB J. 25 (6): 1972-82. -
Dewals, B.G. et al. (2010) IL-4Ralpha-independent expression of mannose receptor and Ym1 by macrophages depends on their IL-10 responsiveness.
PLoS Negl Trop Dis. 4: e689.
View The Latest Product References
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Galès, A. et al. (2010) PPARgamma controls dectin-1 expression required for host antifungal defense against Candida albicans.
PLoS Pathog. 6: e1000714. -
Goodridge, H.S. et al. (2009) Differential use of CARD9 by dectin-1 in macrophages and dendritic cells.
J Immunol. 182: 1146-54. -
Coates, P.J. et al. (2008) Indirect macrophage responses to ionizing radiation: implications for genotype-dependent bystander signaling.
Cancer Res. 68: 450-6. -
Dioszeghy, V. et al. (2008) 12/15-Lipoxygenase regulates the inflammatory response to bacterial products in vivo.
J Immunol. 181: 6514-24. -
Hohl, T.M. (2008) Caspofungin modulates inflammatory responses to Aspergillus fumigatus through stage-specific effects on fungal beta-glucan exposure.
J Infect Dis. 198: 176-85. -
Palma, A.S. et al. (2006) Ligands for the beta-glucan receptor, Dectin-1, assigned using "designer" microarrays of oligosaccharide probes (neoglycolipids) generated from glucan polysaccharides.
J Biol Chem. 281: 5771-9. -
Sindrilaru, A. et al. (2011) An unrestrained proinflammatory M1 macrophage population induced by iron impairs wound healing in humans and mice.
J Clin Invest. 121: 985-97. -
Anandasabapathy, N. et al. (2011) Flt3L controls the development of radiosensitive dendritic cells in the meninges and choroid plexus of the steady-state mouse brain.
J Exp Med. 208 (8): 1695-705. -
Takahara, K. et al. (2012) Efficient capture of Candida albicans and zymosan by SIGNR1 augments TLR2-dependent TNF-α production.
Int Immunol. 24 (2): 89-96. -
Fransen F et al. (2015) BALB/c and C57BL/6 Mice Differ in Polyreactive IgA Abundance, which Impacts the Generation of Antigen-Specific IgA and Microbiota Diversity.
Immunity. 43 (3): 527-40. -
Urso, K. et al. (2016) Anion Exchanger 2 Regulates Dectin-1-Dependent Phagocytosis and Killing of Candida albicans.
PLoS One. 11 (7): e0158893. -
Zhou, J. et al. (2016) Therapeutic targeting of myeloid-derived suppressor cells involves a novel mechanism mediated by clusterin.
Sci Rep. 6: 29521. -
Pinke, K.H. et al. (2016) Mast cells phagocyte Candida albicans and produce nitric oxide by mechanisms involving TLR2 and Dectin-1.
Immunobiology. 221 (2): 220-7. -
Berven, L. et al. (2015) Particulate yeast β-glucan is internalized by RAW 264.7 macrophages and reduces the activity of the tumor-associated protease legumain
Bioactive Carbohydrates and Dietary Fibre. 6 (1): 15-23. -
Walachowski, S. et al. (2016) Triggering Dectin-1-Pathway Alone Is Not Sufficient to Induce Cytokine Production by Murine Macrophages.
PLoS One. 11 (2): e0148464. -
Ferguson, B.J. et al. (2015) The Schistosoma mansoni T2 ribonuclease omega-1 modulates inflammasome-dependent IL-1β secretion in macrophages.
Int J Parasitol. 45 (13): 809-13. -
Masuda, Y. et al. (2015) Soluble β-glucan from Grifola frondosa induces tumor regression in synergy with TLR9 agonist via dendritic cell-mediated immunity.
J Leukoc Biol. 98 (6): 1015-25. -
Baldwin, K.T. et al. (2015) Neuroinflammation triggered by β-glucan/dectin-1 signaling enables CNS axon regeneration.
Proc Natl Acad Sci U S A. 112 (8): 2581-6. -
Quayle K et al. (2015) The TLR2 agonist in polysaccharide-K is a structurally distinct lipid which acts synergistically with the protein-bound β-glucan.
J Nat Med. 69 (2): 198-208. -
Chang, T.H. et al. (2017) Dectin-2 is a primary receptor for NLRP3 inflammasome activation in dendritic cell response to Histoplasma capsulatum.
PLoS Pathog. 13 (7): e1006485. -
Seo, B.S. et al. (2016) Dectin-1 agonist selectively induces IgG1 class switching by LPS-activated mouse B cells.
Immunol Lett. 178: 114-21. -
Thompson, A. et al. (2019) The protective effect of inflammatory monocytes during systemic C. albicans. infection is dependent on collaboration between C-type lectin-like receptors.
PLoS Pathog. 15 (6): e1007850. -
Uno, A. et al. (2021) A novel β-glucan-oligonucleotide complex selectively delivers siRNA to APCs via Dectin-1.
J Control Release. 338: 792-803. -
Deerhake, M.E. et al. (2021) Dectin-1 limits autoimmune neuroinflammation and promotes myeloid cell-astrocyte crosstalk via Card9-independent expression of Oncostatin M.
Immunity. 54 (3): 484-498.e8. -
Niekamp, P. et al. (2021) Sphingomyelin Biosynthesis Is Essential for Phagocytic Signaling during Mycobacterium tuberculosis. Host Cell Entry.
mBio. 12 (1): e03141-20. -
Oh, S. et al. (2022) Pathogen size alters C-type lectin receptor signaling in dendritic cells to influence CD4 Th9 cell differentiation.
Cell Rep. 38 (13): 110567. -
Masuda, Y. et al. (2023) Maitake α-glucan promotes differentiation of monocytic myeloid-derived suppressor cells into M1 macrophages.
Life Sci. 317: 121453. -
Yamashita, H. et al. (2022) Comprehensive expression analysis with cell-type-specific transcriptome in ALS-linked mutant SOD1 mice: Revisiting the active role of glial cells in disease.
Front Cell Neurosci. 16: 1045647. -
Erkelens, M.N. et al. (2020) Intestinal Macrophages Balance Inflammatory Expression Profiles via Vitamin A and Dectin-1-Mediated Signaling.
Front Immunol. 11: 551. -
Zimara, N. et al. (2018) Dectin-1 Positive Dendritic Cells Expand after Infection with Leishmania major Parasites and Represent Promising Targets for Vaccine Development.
Front Immunol. 9: 263.
- Synonyms
- CD369
- RRID
- AB_321142
- UniProt
- Q6QLQ4
- Entrez Gene
- Clec7a
- GO Terms
- GO:0001846 opsonin binding
- GO:0001874 1,3-beta-D-glucan receptor activity
- GO:0001879 detection of yeast
- GO:0002238 response to molecule of fungal origin
- GO:0002752 cell surface pattern recognition receptor signaling pathway
- GO:0016021 integral to membrane
- GO:0005529 sugar binding
- GO:0006910 phagocytosis, recognition
- GO:0006911 phagocytosis, engulfment
- View More GO Terms
- GO:0006954 inflammatory response
- GO:0009897 external side of plasma membrane
- GO:0016337 cell-cell adhesion
- GO:0032760 positive regulation of tumor necrosis factor production
- GO:0045087 innate immune response
- GO:0050766 positive regulation of phagocytosis
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