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CD79b antibody | AT107-2

Rat anti Mouse CD79b:FITC

Product Type
Monoclonal Antibody
Clone
AT107-2
Isotype
IgG1
Specificity
CD79b

Product Code Applications Pack Size List Price Your Price Qty
MCA2209F
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F * 0.1 mg loader
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Rat anti Mouse CD79b antibody, clone AT107-2 recognizes a cytoplasmic region of mouse B-cell antigen receptor complex-associated protein beta chain, also known as B-cell-specific glycoprotein B29, Ig-beta, or Immunoglobulin-associated B29 protein. CD79b is a 228 amino acid, including a 26 signal peptide ~40 kDa type I single pass transmembrane glycoprotein. CD79b is expressed by B lymphocytes and associates with CD79a to form a heterodimer, non-covalently linked to surface immunoglobulin, forming the B-cell receptor (BCR) complex. Rat anti Mouse CD79b antibody, clone AT107-2 also recognizes a homologous region of human CD79b.

Target Species
Mouse
Species Cross-Reactivity
Target SpeciesCross Reactivity
Human
Rat
Pig
Dog
Chicken
Koala
Tasmanian Devil
Cat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% sodium azide (NaN3)
1% bovine serum albumin
Immunogen
Peptide containing 20 amino acid residues from mouse CD79b conjugated to Keyhole Limpet Hemocyanin (KLH).
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Fusion Partners
Spleen cells from immunized rat were fused with cells of the mouse NS-1 myeloma cell line.
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
FITC 490 525
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1 1/50 1/100
  1. 1 Membrane permeabilization is required for this application. The use of Leucoperm (Product Code BUF09) is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10μl of the suggested working dilution to label 1x106 cells in 100μl

References for CD79b antibody

  1. Vendel,A.C. et al (2009) B and T lymphocyte attenuator regulates B cell receptor signaling by targeting Syk and BLNK.
    J Immunol. 182: 1509-17
  2. Kreiss, A. et al. (2009) A histological and immunohistochemical analysis of lymphoid tissues of the Tasmanian devil.
    Anat Rec (Hoboken). 292: 611-20.
  3. Dornan, D. et al. (2009) Therapeutic potential of an anti-CD79b antibody-drug conjugate, anti-CD79b-vc-MMAE, for the treatment of non-Hodgkin lymphoma.
    Blood. 114: 2721-9.
  4. Lau, Q. et al. (2012) Expression and in vitro upregulation of MHCII in koala lymphocytes.
    Vet Immunol Immunopathol. 147: 35-43.
  5. Thalheim, L. et al. (2013) Lymphoma immunophenotype of dogs determined by immunohistochemistry, flow cytometry, and polymerase chain reaction for antigen receptor rearrangements.
    J Vet Intern Med. 27 (6): 1509-16.
  6. McCurdy, P. et al. (2014) Acute lymphoblastic leukemia in a pygmy hippopotamus (Hexaprotodon liberiensis).
    J Zoo Wildl Med. 45 (4): 906-10.
  7. Kandasamy S et al. (2014) Prenatal vitamin A deficiency impairs adaptive immune responses to pentavalent rotavirus vaccine (RotaTeq®) in a neonatal gnotobiotic pig model.
    Vaccine. 32 (7): 816-24.
  8. Ouyang, K. et al. (2015) Evaluation of humoral immune status in porcine epidemic diarrhea virus (PEDV) infected sows under field conditions.
    Vet Res. 46: 140.
  9. View The Latest Product References
  10. Whitney, J.L. et al. (2016) Immunohistochemical Analysis of Leucocyte Subsets in the Sinonasal Mucosa of Cats with Upper Respiratory Tract Aspergillosis.
    J Comp Pathol. 155 (2-3): 130-40.
  11. Poggi, A. et al (2017) Prognostic significance of Ki67 evaluated by flow cytometry in dogs with high-grade B-cell lymphoma.
    Vet Comp Oncol. 15 (2): 431-40.
  12. Bennett, A.L> et al. (2017) Canine acute leukaemia: 50 cases (1989-2014).
    Vet Comp Oncol. 15 (3): 1101-14.
  13. Martini, V. et al. (2018) A retrospective study of flow cytometric characterization of suspected extranodal lymphomas in dogs.
    J Vet Diagn Invest. 30 (6): 830-6.
  14. Tilly, H. et al. (2019) Polatuzumab vedotin in combination with immunochemotherapy in patients with previously untreated diffuse large B-cell lymphoma: an open-label, non-randomised, phase 1b–2 study
    The Lancet Oncology. 20 (7): 998-1010.
  15. Allan, J.N. et al. (2019) CD79b Expression in Richter's Transformation
    Blood. 134 (Supplement_1): 4279.
  16. Borthwick, C.R. et al. (2019) An Examination of the Development and Localization of Key Immune Cells in Developing Pouch Young of the Red-Tailed Phascogale (Phascogale calura).
    Anat Rec (Hoboken). 302 (11): 1985-2002.
  17. Sehn, L.H. et al. (2020) Polatuzumab Vedotin in Relapsed or Refractory Diffuse Large B-Cell Lymphoma.
    J Clin Oncol. 38 (2): 155-65.
  18. Antognoni, M.T. et al. (2021) Non Epitheliotropic B-Cell Lymphoma with Plasmablastic Differentiation vs. Cutaneous Plasmacytosis in a 12-Years-Old Beagle: Case Presentation and Clinical Review.
    Vet Sci. 8 (12): 317.
  19. Herrera, A.F. et al. (2022) Anti-CD79B Antibody-Drug Conjugate DCDS0780A in Patients with B-Cell Non-Hodgkin Lymphoma: Phase 1 Dose-Escalation Study.
    Clin Cancer Res. 28 (7):1294-301.

Further Reading

  1. Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.
    Vet Res. 39: 54.

Synonyms
B29
RRID
AB_324172
UniProt
P15530
Entrez Gene
Cd79b
GO Terms
GO:0005515 protein binding
GO:0016021 integral to membrane
GO:0004888 transmembrane receptor activity
GO:0009897 external side of plasma membrane
GO:0019815 B cell receptor complex
GO:0050853 B cell receptor signaling pathway

MCA2209F

160355 166783 1709

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