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CD13 antibody | R3-63

Product Code Applications Pack Size List Price Your Price Qty
MCA2183PET
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SDS Safety Datasheet SDS
F 25 Tests loader
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MCA2183PE
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SDS Safety Datasheet SDS
F 100 Tests loader
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Rat anti Mouse CD13 antibody, clone R3-63 recognizes mouse aminopeptidase N (APN), a cell surface protein homologous with human CD13. In the mouse, CD13 is a non-covalently linked homodimer of approximately 150 kDa subunits expressed by a variety of cells including monocytes, macrophages, dendritic cell and veiled cells.

Rat anti Mouse CD13 antibody, clone R3-63 has been reported to block mouse APN enzyme activity (Hansen et al. 1993).

Target Species
Mouse
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution
MCA2183PET: Reconstitute in 0.25 ml distilled water
MCA2183PE: Reconstitute with 1.0 ml distilled water
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% sodium azide (NaN3)
1% bovine serum albumin
5% sucrose
Immunogen
Mouse intestinal APN
Fusion Partners
Spleen cells from immunized mice were fused with cells of the IR983F rat myeloma cell line.
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
RPE 488nm laser 496 578
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Prior to reconstitution store at +4°C. Following reconstitution store at +4°C.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10μl of the suggested working dilution to label 106 cells in 100μl.
The Fc region of monoclonal antibodies may bind to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/BUF041B).

How to Use the Spectraviewer

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Description Product Code Applications Pack Size List Price Your Price Quantity
Rat IgG2a Negative Control:RPE MCA1212PE F 100 Tests
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Description Rat IgG2a Negative Control:RPE

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse Seroblock FcR BUF041A F 0.1 mg loader
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Description Mouse Seroblock FcR
Mouse Seroblock FcR BUF041B F 0.5 mg loader
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Description Mouse Seroblock FcR

References for CD13 antibody

  1. Kamoun, W.S. et al. (2009) Edema control by cediranib, a vascular endothelial growth factor receptor-targeted kinase inhibitor, prolongs survival despite persistent brain tumor growth in mice.
    J Clin Oncol. 27: 2542-52.
  2. Hansen, A.S. et al. (1993) A mouse aminopeptidase N is a marker for antigen-presenting cells and appears to be co-expressed with major histocompatibility complex class II molecules.
    Eur J Immunol. 23 (9): 2358-64.
  3. Larsen, S.L. et al. (1996) T cell responses affected by aminopeptidase N (CD13)-mediated trimming of major histocompatibility complex class II-bound peptides.
    J Exp Med. 184 (1): 183-9.
  4. Rangel, R. et al. (2007) Impaired angiogenesis in aminopeptidase N-null mice.
    Proc Natl Acad Sci U S A. 104: 4588-93.
  5. Lahdenranta, J. et al. (2007) Treatment of hypoxia-induced retinopathy with targeted proapoptotic peptidomimetic in a mouse model of disease.
    FASEB J. 21: 3272-8.
  6. Li, P. et al. (2010) Use of adenoviral vectors to target chemotherapy to tumor vascular endothelial cells suppresses growth of breast cancer and melanoma.
    Mol Ther. 18: 921-8.
  7. van Deventer, H.W. et al. (2008) C-C chemokine receptor 5 on pulmonary fibrocytes facilitates migration and promotes metastasis via matrix metalloproteinase 9.
    Am J Pathol. 173: 253-64.
  8. Gabrilovac, J. et al. (2011) Expression, regulation and functional activities of aminopeptidase N (EC 3.4.11.2; APN; CD13) on murine macrophage J774 cell line.
    Immunobiology. 216: 132-44.
  9. View The Latest Product References
  10. Ozawa, M.G. et al. (2008) Beyond receptor expression levels: the relevance of target accessibility in ligand-directed pharmacodelivery systems.
    Trends Cardiovasc Med. 18: 126-32.
  11. Bertilaccio, M.T. et al. (2008) Vasculature-targeted tumor necrosis factor-alpha increases the therapeutic index of doxorubicin against prostate cancer.
    Prostate. 68: 1105-15.
  12. Boström, M. et al. (2014) The hippocampal neurovascular niche during normal development and after irradiation to the juvenile mouse brain.
    Int J Radiat Biol. 90: 778-89.
  13. Mayer-Barber, K.D. et al. (2011) Innate and adaptive interferons suppress IL-1α and IL-1β production by distinct pulmonary myeloid subsets during Mycobacterium tuberculosis infection.
    Immunity. 35: 1023-34.
  14. Winnicka, B. et al. (2010) CD13 is dispensable for normal hematopoiesis and myeloid cell functions in the mouse.
    J Leukoc Biol. 88: 347-59.
  15. Ridder, D.A. et al. (2015) Brain endothelial TAK1 and NEMO safeguard the neurovascular unit.
    J Exp Med. 212 (10): 1529-49.
  16. Vanlandewijck, M. et al. (2015) Functional Characterization of Germline Mutations in PDGFB and PDGFRB in Primary Familial Brain Calcification.
    PLoS One. 10 (11): e0143407.
  17. Körbelin J et al. (2016) A brain microvasculature endothelial cell-specific viral vector with the potential to treat neurovascular and neurological diseases.
    EMBO Mol Med. 8 (6): 609-25.
  18. Zotz, J.S. et al. (2016) CD13/aminopeptidase N is a negative regulator of mast cell activation.
    FASEB J. 30 (6): 2225-35.
  19. Sung, S.J. et al. (2017) Proximal Tubule CD73 Is Critical in Renal Ischemia-Reperfusion Injury Protection.
    J Am Soc Nephrol. 28 (3): 888-902.
  20. Yanagida, K. et al. (2017) Size-selective opening of the blood-brain barrier by targeting endothelial sphingosine 1-phosphate receptor 1.
    Proc Natl Acad Sci U S A. 114 (17): 4531-6.
  21. Elabi, O. et al. (2021) Human α-synuclein overexpression in a mouse model of Parkinson's disease leads to vascular pathology, blood brain barrier leakage and pericyte activation.
    Sci Rep. 11 (1): 1120.
  22. Kato, T. et al. (2020) Excessive Production of Transforming Growth Factor β1 Causes Mural Cell Depletion From Cerebral Small Vessels.
    Front Aging Neurosci. 12: 151.
  23. Chen, X. et al. (2023) Functional gene delivery to and across brain vasculature of systemic AAVs with endothelial-specific tropism in rodents and broad tropism in primates.
    Nat Commun. 14 (1): 3345.

Immunofluorescence

Synonyms
Aminopeptidase N
RRID
AB_324138
UniProt
P97449
Entrez Gene
Anpep
GO Terms
GO:0001525 angiogenesis
GO:0004177 aminopeptidase activity
GO:0008237 metallopeptidase activity
GO:0016021 integral to membrane
GO:0008270 zinc ion binding
GO:0030154 cell differentiation

MCA2183PET

INN1607

MCA2183PE

INN1607

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