CD13 antibody | R3-63
Rat anti Mouse CD13:Alexa Fluor® 647
- Product Type
- Monoclonal Antibody
- Clone
- R3-63
- Isotype
- IgG2a
- Specificity
- CD13
Rat anti Mouse CD13 antibody, clone R3-63 recognizes mouse aminopeptidase N (APN), a cell surface protein homologous with human CD13. In the mouse, CD13 is a non-covalently linked homodimer of approximately 150 kDa subunits expressed by a variety of cells including monocytes, macrophages, dendritic cell and veiled cells. Rat anti Mouse CD13 antibody, clone R3-63 has been reported to block mouse APN enzyme activity (Hansen et al. 1993). |
- Target Species
- Mouse
- Product Form
- Purified IgG conjugated to Alexa Fluor® 647 - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
1% bovine serum albumin - Immunogen
- Mouse intestinal APN
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Fusion Partners
- Spleen cells from immunized mice were fused with cells of the IR983F rat myeloma cell line.
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) Alexa Fluor®647 650 665 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | Neat | 1/5 |
Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
---|---|---|---|---|---|---|---|
Rat IgG2a Negative Control:Alexa Fluor® 647 | MCA1212A647 | F | 100 Tests/1ml | Log in | |||
List Price | Your Price | ||||||
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Description | Rat IgG2a Negative Control:Alexa Fluor® 647 |
Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
---|---|---|---|---|---|---|---|
Mouse Seroblock FcR | BUF041A | F | 0.1 mg | Log in | |||
List Price | Your Price | ||||||
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Description | Mouse Seroblock FcR | ||||||
Mouse Seroblock FcR | BUF041B | F | 0.5 mg | Log in | |||
List Price | Your Price | ||||||
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Description | Mouse Seroblock FcR |
References for CD13 antibody
-
Kamoun, W.S. et al. (2009) Edema control by cediranib, a vascular endothelial growth factor receptor-targeted kinase inhibitor, prolongs survival despite persistent brain tumor growth in mice.
J Clin Oncol. 27: 2542-52. -
Hansen, A.S. et al. (1993) A mouse aminopeptidase N is a marker for antigen-presenting cells and appears to be co-expressed with major histocompatibility complex class II molecules.
Eur J Immunol. 23 (9): 2358-64. -
Larsen, S.L. et al. (1996) T cell responses affected by aminopeptidase N (CD13)-mediated trimming of major histocompatibility complex class II-bound peptides.
J Exp Med. 184 (1): 183-9. -
Rangel, R. et al. (2007) Impaired angiogenesis in aminopeptidase N-null mice.
Proc Natl Acad Sci U S A. 104: 4588-93. -
Lahdenranta, J. et al. (2007) Treatment of hypoxia-induced retinopathy with targeted proapoptotic peptidomimetic in a mouse model of disease.
FASEB J. 21: 3272-8. -
Li, P. et al. (2010) Use of adenoviral vectors to target chemotherapy to tumor vascular endothelial cells suppresses growth of breast cancer and melanoma.
Mol Ther. 18: 921-8. -
van Deventer, H.W. et al. (2008) C-C chemokine receptor 5 on pulmonary fibrocytes facilitates migration and promotes metastasis via matrix metalloproteinase 9.
Am J Pathol. 173: 253-64. -
Gabrilovac, J. et al. (2011) Expression, regulation and functional activities of aminopeptidase N (EC 3.4.11.2; APN; CD13) on murine macrophage J774 cell line.
Immunobiology. 216: 132-44.
View The Latest Product References
-
Ozawa, M.G. et al. (2008) Beyond receptor expression levels: the relevance of target accessibility in ligand-directed pharmacodelivery systems.
Trends Cardiovasc Med. 18: 126-32. -
Bertilaccio, M.T. et al. (2008) Vasculature-targeted tumor necrosis factor-alpha increases the therapeutic index of doxorubicin against prostate cancer.
Prostate. 68: 1105-15. -
Boström, M. et al. (2014) The hippocampal neurovascular niche during normal development and after irradiation to the juvenile mouse brain.
Int J Radiat Biol. 90: 778-89. -
Mayer-Barber, K.D. et al. (2011) Innate and adaptive interferons suppress IL-1α and IL-1β production by distinct pulmonary myeloid subsets during Mycobacterium tuberculosis infection.
Immunity. 35: 1023-34. -
Winnicka, B. et al. (2010) CD13 is dispensable for normal hematopoiesis and myeloid cell functions in the mouse.
J Leukoc Biol. 88: 347-59. -
Ridder, D.A. et al. (2015) Brain endothelial TAK1 and NEMO safeguard the neurovascular unit.
J Exp Med. 212 (10): 1529-49. -
Vanlandewijck, M. et al. (2015) Functional Characterization of Germline Mutations in PDGFB and PDGFRB in Primary Familial Brain Calcification.
PLoS One. 10 (11): e0143407. -
Körbelin J et al. (2016) A brain microvasculature endothelial cell-specific viral vector with the potential to treat neurovascular and neurological diseases.
EMBO Mol Med. 8 (6): 609-25. -
Zotz, J.S.
et al. (2016) CD13/aminopeptidase N is a negative regulator of mast cell activation.
FASEB J. 30 (6): 2225-35. -
Sung, S.J. et al. (2017) Proximal Tubule CD73 Is Critical in Renal Ischemia-Reperfusion Injury Protection.
J Am Soc Nephrol. 28 (3): 888-902. -
Yanagida, K. et al. (2017) Size-selective opening of the blood-brain barrier by targeting endothelial sphingosine 1-phosphate receptor 1.
Proc Natl Acad Sci U S A. 114 (17): 4531-6. -
Elabi, O. et al. (2021) Human α-synuclein overexpression in a mouse model of Parkinson's disease leads to vascular pathology, blood brain barrier leakage and pericyte activation.
Sci Rep. 11 (1): 1120. -
Kato, T. et al. (2020) Excessive Production of Transforming Growth Factor β1 Causes Mural Cell Depletion From Cerebral Small Vessels.
Front Aging Neurosci. 12: 151. -
Chen, X. et al. (2023) Functional gene delivery to and across brain vasculature of systemic AAVs with endothelial-specific tropism in rodents and broad tropism in primates.
Nat Commun. 14 (1): 3345.
- Synonyms
- Aminopeptidase N
- RRID
- AB_324883
- UniProt
- P97449
- Entrez Gene
- Anpep
- GO Terms
- GO:0001525 angiogenesis
- GO:0004177 aminopeptidase activity
- GO:0008237 metallopeptidase activity
- GO:0016021 integral to membrane
- GO:0008270 zinc ion binding
- GO:0030154 cell differentiation
MCA2183A647T
150994 170202MCA2183A647
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