DAXX antibody | DAXX-01
Mouse anti Human DAXX antibody, clone DAXX-01 (MCA2143) used for the detection of DAXX in iSLK cells by immunofluorescence and western blotting.
Image caption:
KSHV infection antagonizes the ND10 component ATRX. iSLK cells were seeded onto glass slides, infected with KSHV strain rKSHV.219 for 24A: single staining for PML (c), Sp100 (g) and ATRX (l) B: double staining for ATRX (c) and Daxx (d). Arrows indicate infected cells. C: iSLK cells were infected with KSHV strain rKSHV.219 with high MOI and harvested 20 h post infection. Whole cell lysates were prepared and expression of ND10 components analyzed by immunoblotting.
From: Full F, Jungnickl D, Reuter N, Bogner E, Brulois K, Scholz B, et al. (2014)
Kaposi's Sarcoma Associated Herpesvirus Tegument Protein ORF75 Is Essential for Viral Lytic Replication and Plays a Critical Role in the Antagonization of ND10-Instituted Intrinsic Immunity.
PLoS Pathog 10(1): e1003863.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Human DAXX antibody, clone DAXX-01 (MCA2143) used for the detection of DAXX in human foreskin fibroblasts by western blotting.
Image caption:
KSHV ORF75 is sufficient for antagonization of ATRX and Sp100. A: HFF cells were transfected with a pcDNA-orf75-myc expression construct. Cells were analyzed by immunofluorescence with a confocal microscope, and at least 50 individual cells per each box analyzed by Fiji. Statistical analyses were conducted using the unpaired t test for SP100 and PML, and one way ANOVA test for multiple comparisons for Daxx and ATRX, respectively, of GraphPad Prism 6 for Windows. **p<0.01; *** p<0.001. B: 293T cells were transfected with increasing amounts of ORF75 and endogenous expression of ATRX and Daxx was analyzed by immunoblot. C: 293T cells were cotransfected with constant levels of Sp100 and increasing amounts of ORF75 and expression of Sp100 and Daxx levels analyzed by immunoblot.
From: Full F, Jungnickl D, Reuter N, Bogner E, Brulois K, Scholz B, et al. (2014)
Kaposi's Sarcoma Associated Herpesvirus Tegument Protein ORF75 Is Essential for Viral Lytic Replication and Plays a Critical Role in the Antagonization of ND10-Instituted Intrinsic Immunity.
PLoS Pathog 10(1): e1003863.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Human DAXX antibody, clone DAXX-01 (MCA2143) used for the detection of DAXX in human foreskin fibroblasts by immunofluorescence.
Image caption:
Diffuse localization of ATRX after knockdown of Daxx. HFF cells carrying retroviral knockdown shRNA vectors targeting Daxx or PML were immunostained with respective antibodies. ND10 accumulation as shown by colocalization (c) with PML (a) of ATRX (b) is lost in Daxx-kd cells (e,f) while PML (d) remains in ND-10 structures. In contrast, knockdown of PML (g–i) results in dispersal of ND10 and seemingly a partial colocalization (i) of Daxx (g) and ATRX (h) into smaller structures; most of Daxx and ATRX proteins are not colocalized.
From: Full F, Jungnickl D, Reuter N, Bogner E, Brulois K, Scholz B, et al. (2014)
Kaposi's Sarcoma Associated Herpesvirus Tegument Protein ORF75 Is Essential for Viral Lytic Replication and Plays a Critical Role in the Antagonization of ND10-Instituted Intrinsic Immunity.
PLoS Pathog 10(1): e1003863.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Human DAXX
- Product Type
- Monoclonal Antibody
- Clone
- DAXX-01
- Isotype
- IgG1
- Specificity
- DAXX
Antibody Quality and Performance Guarantee
| Mouse anti Human DAXX antibody, clone DAXX-01 recognizes human Death domain-associated protein 6, also known as DAXX, ETS1-associated protein 1, EAP1 or Fas death domain-associated protein. DAXX is a 740 amino acid ~81 kDa nucleoplasmic protein. DAXX is a regulator of apoptosis implicated in Fas and TGF beta induced apoptosis. DAXX is predominantly a nuclear protein, where it as a repressor of EtsI and Pax3 transactivation. When in association with ASK1 kinase DAXX translocates from the nucleus to the cytoplasm. |
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- < 0.1% sodium azide (NaN3)
- Immunogen
- Recombinant C-terminal DAXX protein (aa 558-740).
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunofluorescence |  |
||
| Immunohistology - Frozen | |
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| Western Blotting | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
References for DAXX antibody
-
Boehrer, S. et al. (2005) Daxx overexpression in T-lymphoblastic Jurkat cellsenhances caspase-dependent death receptor- and drug-induced apoptosis in distinct ways.
Cell Signal. 17:581-95. -
Tavalai, N. et al. (2006) Evidence for a role of the cellular ND10 protein PML in mediating intrinsic immunity against human cytomegalovirus infections.
J Virol. 80: 8006-18. -
Sourvinos, G. et al. (2007) Recruitment of human cytomegalovirus immediate-early 2 protein onto parental viral genomes in association with ND10 in live-infected cells.
J Virol. 81: 10123-36. -
Tavalai, N. et al. (2008) Insertion of an EYFP-pp71 (UL82) coding sequence into the human cytomegalovirus genome results in a recombinant virus with enhanced viral growth.
J Virol. 82: 10543-55. -
Full, F. et al. (2012) Herpesvirus saimiri antagonizes nuclear domain 10-instituted intrinsic immunity via an ORF3-mediated selective degradation of cellular protein Sp100.
J Virol. 86 (7): 3541-53. -
Full, F. et al. (2014) Kaposi's Sarcoma Associated Herpesvirus Tegument Protein ORF75 Is Essential for Viral Lytic Replication and Plays a Critical Role in the Antagonization of ND10-Instituted Intrinsic Immunity.
PLoS Pathog. 10(1):e1003863. -
Brown, J.R. et al. (2016) SUMO Ligase Protein Inhibitor of Activated STAT1 (PIAS1) Is a Constituent Promyelocytic Leukemia Nuclear Body Protein That Contributes to the Intrinsic Antiviral Immune Response to Herpes Simplex Virus 1.
J Virol. 90 (13): 5939-52.
- Synonyms
- Death DOMAIN-ASSOCIATED Protein 6
- RRID
- AB_2088900
- UniProt
- Q9UER7
- Entrez Gene
- DAXX
- GO Terms
- GO:0000775 chromosome, centromeric region
- GO:0002039 p53 binding
- GO:0006915 apoptosis
- GO:0005057 receptor signaling protein activity
- GO:0005730 nucleolus
- GO:0006350 transcription
- GO:0007257 activation of JUN kinase activity
- GO:0008134 transcription factor binding
- GO:0008625 induction of apoptosis via death domain receptors
- View More GO Terms
- GO:0016481 negative regulation of transcription
- GO:0016605 PML body
- GO:0031072 heat shock protein binding
- GO:0030521 androgen receptor signaling pathway
- GO:0031396 regulation of protein ubiquitination
- GO:0031625 ubiquitin protein ligase binding
- GO:0042803 protein homodimerization activity
- GO:0044419 interspecies interaction between organisms
- GO:0045892 negative regulation of transcription, DNA-dependent
- GO:0047485 protein N-terminus binding
- GO:0050681 androgen receptor binding
MCA2143
164835
170511
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