CD25 antibody | MEM-181
Mouse anti Human CD25:Alexa Fluor® 488
- Product Type
- Monoclonal Antibody
- Clone
- MEM-181
- Isotype
- IgG1
- Specificity
- CD25
Mouse anti Human CD25, clone MEM-181 recognizes the ~55 kDa alpha subunit of the human IL-2 receptor, also known as p55 or TAC antigen, CD25 is a type 1 transmembrane protein with two Sushi domains, also known as short concensus repeats (SCRs) or complement control protein (CCP) modules (Norman et al. 1991) located within its extracellular domain. The IL-2 receptor exists in three forms. A high affinity form consisting of a non-covalently linked heterodimer composed of the alpha subunit (CD25) and the IL-2 receptor beta subunit also known as CD122 or p75, a medium affinity beta subunit (CD122) monomer or a low affinity alpha (CD25) subunit monomer. CD25 is expressed by activated T lymphocytes and activated B lymphocytes responding to antigen or mitogen stimulation. CD25 is also expressed in some thymocytes and oligodendrocytes. In disease, elevated expression of CD25 in noted in a number of chronic inflammatory conditions, tuberculoid leprosy patients demonstrate markedly elevated levels of circulating CD25high FoxP3+ regulatory T cells (T-regs) (Attia et al. 2010). Elevated levels of CD25 antigen expression are often seen in cases of non-Hodgkin 's lymphoma and diffuse large B cell lymphoma (Fujiwara et al.2013). |
- Target Species
- Human
- Product Form
- Purified IgG conjugated to Alexa Fluor® 488 - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
1% bovine serum albumin - Immunogen
- Human PHA blasts; day 3 of culture.
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the mouse P3.X63 Ag8.653 myeloma cell line.
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) Alexa Fluor®488 495 519 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | Neat | 1/10 |
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells in 100μl
Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
---|---|---|---|---|---|---|---|
Mouse IgG1 Negative Control:Alexa Fluor® 488 | MCA928A488 | F | 100 Tests/1ml | Log in | |||
List Price | Your Price | ||||||
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Description | Mouse IgG1 Negative Control:Alexa Fluor® 488 |
Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
---|---|---|---|---|---|---|---|
Human Seroblock | BUF070A | F | 50 Test | Log in | |||
List Price | Your Price | ||||||
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Description | Human Seroblock | ||||||
Human Seroblock | BUF070B | F | 200 Test | Log in | |||
List Price | Your Price | ||||||
Log in | |||||||
Description | Human Seroblock |
References for CD25 antibody
-
Prager, E. et al. (2001) Induction of hyporesponsiveness and impaired T lymphocyte activation by the CD31 receptor:ligand pathway in T cells.
J Immunol. 166 (4): 2364-71. -
Thorborn, G. et al. (2010) Increased sensitivity of CD4+ T-effector cells to CD4+CD25+ Treg suppression compensates for reduced Treg number in asymptomatic HIV-1 infection.
PLoS One. 5: e9254. -
Cutler, A.J. et al. (2010) Umbilical cord-derived mesenchymal stromal cells modulate monocyte function to suppress T cell proliferation.
J Immunol. 185: 6617-23. -
Lawson, J.M. et al. (2008) Increased resistance to CD4+CD25hi regulatory T cell-mediated suppression in patients with type 1 diabetes.
Clin Exp Immunol. 154: 353-9. -
Holderness, J. et al. (2007) Select plant tannins induce IL-2Ralpha up-regulation and augment cell division in gammadelta T cells.
J Immunol. 179: 6468-78. -
Zhang, Y. et al. (2013) Accelerated in vivo proliferation of memory phenotype CD4+ T-cells in human HIV-1 infection irrespective of viral chemokine co-receptor tropism.
PLoS Pathog. 9 (4): e1003310. -
Nocentini, G. et al. (2014) Expansion of regulatory GITR + CD25 Low/- CD4 + T cells in systemic lupus erythematosus patients.
Arthritis Res Ther. 16: 444. -
Soukup, K. et al. (2015) The MAPK-Activated Kinase MK2 Attenuates Dendritic Cell-Mediated Th1 Differentiation and Autoimmune Encephalomyelitis.
J Immunol. 195 (2): 541-52.
View The Latest Product References
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Kusunoki, Y. et al. (2010) T-cell immunosenescence and inflammatory response in atomic bomb survivors.
Radiat Res. 174 (6): 870-6. -
Bughani, U. et al. (2017) T cell activation and differentiation is modulated by a CD6 domain 1 antibody Itolizumab.
PLoS One. 12 (7): e0180088. -
Knutson, K.L. et al. (2015) Regulatory T cells, inherited variation, and clinical outcome in epithelial ovarian cancer.
Cancer Immunol Immunother. 64 (12): 1495-504. -
Boland, J.W. et al. (2014) A preliminary evaluation of the effects of opioids on innate and adaptive human in vitro immune function.
BMJ Support Palliat Care. 4 (4): 357-67. -
Luger, R. et al. (2013) Toll-like receptor 4 engagement drives differentiation of human and murine dendritic cells from a pro- into an anti-inflammatory mode.
PLoS One. 8 (2): e54879. -
Rezalotfi, A. et al. (2020) Gastrospheres as a Model of Gastric Cancer Stem Cells Skew Th17/Treg Balance toward Antitumor Th17 Cells.
J Immunol Res. 2020: 6261814. -
Thymianou, S et al. (2019) MBP7285 on Human Tcell Activation
Mobile health Knoledge 21 Jul
- Synonyms
- IL-2R Alpha Chain
- RRID
- AB_566671
- UniProt
- P01589
- Entrez Gene
- IL2RA
- GO Terms
- GO:0016021 integral to membrane
- GO:0004911 interleukin-2 receptor activity
- GO:0008283 cell proliferation
MCA2127A488
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