Aquaporin 1 antibody | 7D11
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Mouse anti Aquaporin 1
- Product Type
- Monoclonal Antibody
- Clone
- 7D11
- Isotype
- IgG1
- Specificity
- Aquaporin 1
Antibody Quality and Performance Guarantee
| Mouse anti Rat aquaporin 1 antibody, clone 7D11 recognizes an epitope within an extracellular region of the water-specific channel aquaporin 1 (also known as AQP1 or CHIP-28) Aquaporin 1 is a 28kDa integral membrane protein which was originally identified in red blood cells and renal proximal tubules. AQP1 is also expressed by the choroid plexus and various other tissues. The glycosylated forms of AQP1 range between 40-60kDa in mass. |
- Target Species
- Rat
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Human - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
1% bovine serum albumin - Immunogen
- Synthetic peptide corresponding to amino acids 198-208 of rat aquaporin 1.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunized Balb/c mice were fused with the cells of Sp-2/0 Ag14 mouse myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
1/1000 | 1/10000 |
| Flow Cytometry | |
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| Immunohistology - Paraffin 1 | |
||
| Western Blotting | |
1/1000 | 1/2000 |
- 1 This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA1209 | F | 0.1 mg |
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Log in | ||
| List Price | Your Price | ||||||
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| Description | Mouse IgG1 Negative Control | ||||||
Source Reference
-
Nagy G et al. (2002) Development and characterisation of a monoclonal antibody family against aquaporin 1 (AQP1) and aquaporin 4 (AQP4).
Pathol Oncol Res. 8 (2): 115-24.
References for Aquaporin 1 antibody
-
Bódis, B. et al. (2001) Active water selective channels in the stomach: investigation of aquaporins after ethanol and capsaicin treatment in rats.
J Physiol Paris. 95 (1-6): 271-5. -
Felszeghy, S. et al. (2004) Expression of aquaporin isoforms during human and mouse tooth development.
Arch Oral Biol. 49: 247-57. -
Cabral, P.D and Herrera, M. (2012) Membrane-associated aquaporin-1 facilitates osmotically driven water flux across the basolateral membrane of the thick ascending limb.
Am J Physiol Renal Physiol. 303: F621-9.
- Synonyms
- AQP1
- RRID
- AB_2056833
- UniProt
- P29975
- Entrez Gene
- Aqp1
- GO Terms
- GO:0005515 protein binding
- GO:0016021 integral to membrane
- GO:0005903 brush border
- GO:0010592 positive regulation of lamellipodium assembly
- GO:0010634 positive regulation of epithelial cell migration
- GO:0015168 glycerol transmembrane transporter activity
- GO:0015250 water channel activity
- GO:0016323 basolateral plasma membrane
- GO:0016324 apical plasma membrane
- View More GO Terms
- GO:0021670 lateral ventricle development
- GO:0032809 neuronal cell body membrane
- GO:0042538 hyperosmotic salinity response
- GO:0043627 response to estrogen stimulus
- GO:0043679 axon terminus
MCA2100
149634
180524
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