CD11c antibody | BU15
Mouse anti Human CD11c:Alexa Fluor® 647
- Product Type
- Monoclonal Antibody
- Clone
- BU15
- Isotype
- IgG1
- Specificity
- CD11c
Mouse anti Human CD11c antibody, clone Bu15 recognizes the integrin alphax subunit (ITGAX), a ~150 kDa glycoprotein also known as CD11 antigen-like family member C or Leu M5, CD11c is expressed by macrophages, monocytes, NK cells and most dendritic cells (Kohrgruber et al. 1999). CD11c is also expressed at a lower level by granulocytes. CD11c forms a heterodimeric integral membrane protein with the integrin beta 2 chain to form the leukocyte specific integrin 'inactivated-C3b receptor 4'. CD11c interacts with a number of ligands including the G-P-R sequence in fibrinogen, ICAM-1 (Frick et al. 2005), iC3b ( CD11c is expressed by hairy cell leukaemia cells (Goodman et al. 2003; Nicolaou et al. 2003). |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Cynomolgus monkey - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to Alexa Fluor® 647- liquid
- Preparation
- Purified IgG prepared from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
1% bovine serum albumin - Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) Alexa Fluor®647 650 665 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | Neat | 1/10 |
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells in 100μl
Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
---|---|---|---|---|---|---|---|
Mouse IgG1 Negative Control:Alexa Fluor® 647 | MCA928A647 | F | 100 Tests/1ml | Log in | |||
List Price | Your Price | ||||||
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Description | Mouse IgG1 Negative Control:Alexa Fluor® 647 |
Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
---|---|---|---|---|---|---|---|
Human Seroblock | BUF070A | F | 50 Test | Log in | |||
List Price | Your Price | ||||||
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Description | Human Seroblock | ||||||
Human Seroblock | BUF070B | F | 200 Test | Log in | |||
List Price | Your Price | ||||||
Log in | |||||||
Description | Human Seroblock |
References for CD11c antibody
-
Hogg, N. et al. (1986) The p150,95 molecule is a marker of human mononuclear phagocytes: comparison with expression of class II molecules.
Eur J Immunol. 16 (3): 240-8. -
Levy, O. et al. (2003) Critical role of the complement system in group B streptococcus-induced tumor necrosis factor alpha release.
Infect Immun. 71: 6344-53. -
Newman, K.C. et al. (2006) Cross-talk with myeloid accessory cells regulates human natural killer cell interferon-gamma responses to malaria.
PLoS Pathog. 2: e118. -
Brown, D.P. et al. (2009) The inhibitory receptor LILRB4 (ILT3) modulates antigen presenting cell phenotype and, along with LILRB2 (ILT4), is upregulated in response to Salmonella infection.
BMC Immunol. 10: 56. -
Silk, K.M. et al. (2012) Rapamycin conditioning of dendritic cells differentiated from human ES cells promotes a tolerogenic phenotype.
J Biomed Biotechnol. 2012:172420. -
Herman, S. et al. (2012) Regulatory T cells form stable and long-lasting cell cluster with myeloid dendritic cells (DC).
Int Immunol. 24 (7): 417-26. -
Xie, Z. et al. (2016) Human umbilical cord-derived mesenchymal stem cells elicit macrophages into an anti-inflammatory phenotype to alleviate insulin resistance in type 2 diabetic rats.
Stem Cells. 34 (3): 627-39. -
Schroeder JH et al. (2017) Brugia malayi microfilariae adhere to human vascular endothelial cells in a C3-dependent manner.
PLoS Negl Trop Dis. 11 (5): e0005592.
View The Latest Product References
-
Simões, R.D. et al. (2019) Effects of Regulatory T Cell Depletion on NK Cell Responses against Listeria monocytogenes in Feline Immunodeficiency Virus Infected Cats.
Viruses. 11 (11)Oct 24 [Epub ahead of print].
Further Reading
-
Larson, R.S. & Springer, T.A. (1990) Structure and function of leukocyte integrins.
Immunol Rev. 114: 181-217. -
Loike, J.D. et al. (1991) CD11c/CD18 on neutrophils recognizes a domain at the N terminus of the A alpha chain of fibrinogen.
Proc Natl Acad Sci U S A. 88 (3): 1044-8. -
Sanchez-Madrid, F. and Corbi, A.L. (1992) Leukocyte integrins: structure, function and regulation of their activity.
Seminars Cell Biol. 3: 199-210.
- Synonyms
- Integrin Alpha X Chain
- RRID
- AB_324912
- UniProt
- P20702
- Entrez Gene
- ITGAX
- GO Terms
- GO:0005515 protein binding
- GO:0007596 blood coagulation
- GO:0007155 cell adhesion
- GO:0004872 receptor activity
- GO:0008305 integrin complex
- GO:0007229 integrin-mediated signaling pathway
- GO:0009887 organ morphogenesis
- GO:0050900 leukocyte migration
MCA2087A647
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