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CD284 antibody | HTA125

Mouse anti Human CD284:Alexa Fluor® 647

Product Type
Monoclonal Antibody
Clone
HTA125
Isotype
IgG2a
Specificity
CD284

Product Code Applications Pack Size List Price Your Price Qty
MCA2061A647T
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 25 Tests/0.25ml loader
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MCA2061A647
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 100 Tests/1ml loader
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Mouse anti Human CD284 antibody, clone HTA125 recognizes the human Toll like receptor 4 (TLR4) cell surface antigen.

TLR4, also known as CD284, has been demonstrated to act as a receptor for LPS on human monocytes and macrophages. TLR4 signalling of LPS stimulation requires the presence of the MD-2 molecule.

TLR4 is weakly expressed by resting cells, but is upregulated following stimulation with LPS.

This antibody has been demonstrated to block activation of monocytes with LPS. The use of a preservative free format of Mouse anti Human CD284 antibody, clone HTA125 (MCA2061EL ) is recommended for functional assays.

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rhesus Monkey
Guinea Pig
Pig
Dog
Bovine
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Ig Fraction conjugated to Alexa Fluor® 647 - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% sodium azide (NaN3)
1% bovine serum albumin
Immunogen
Ba/F3 cell line expressing TLR4 (CD284).
Approx. Protein Concentrations
IgG concentration 0.05 mg/ml
Fusion Partners
Spleen cells from immunised Balb/c mice were fused with cells of the mouse SP2/0 myeloma cell line.
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
Alexa Fluor®647 650 665
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/5
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10μl of the suggested working dilution to label 106 cells or 100μl whole blood

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG2a Negative Control:Alexa Fluor® 647 MCA929A647 F 100 Tests/1ml loader
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Description Mouse IgG2a Negative Control:Alexa Fluor® 647

Description Product Code Applications Pack Size List Price Your Price Quantity
Human Seroblock BUF070A F 50 Test
List Price Your Price
Description Human Seroblock
Human Seroblock BUF070B F 200 Test
List Price Your Price
Description Human Seroblock

References for CD284 antibody

  1. Shimazu, R. et al. (1999) MD-2, a molecule that confers lipopolysaccharide responsiveness on Toll-like receptor 4.
    J Exp Med. 189 (11): 1777-82.
  2. Sugawara, S. et al. (2000) Proteolysis of human monocyte CD14 by cysteine proteinases (gingipains) from Porphyromonas gingivalis leading to lipopolysaccharide hyporesponsiveness.
    J Immunol. 165: 411-8.
  3. Yang, S. et al. (2001) Synergistic effect of muramyldipeptide with lipopolysaccharide or lipoteichoic acid to induce inflammatory cytokines in human monocytic cells in culture.
    Infect Immun. 69 (4): 2045-53.
  4. Kawahara T et al. (2001) Type I Helicobacter pylori lipopolysaccharide stimulates toll-like receptor 4 and activates mitogen oxidase 1 in gastric pit cells.
    Infect Immun. 69 (7): 4382-9.
  5. Baumgarten, G. et al. (2001) In vivo expression of proinflammatory mediators in the adult heart after endotoxin administration: the role of toll-like receptor-4.
    J Infect Dis. 183: 1617-24.
  6. Medvedev, A.E. et al. (2001) Induction of tolerance to lipopolysaccharide and mycobacterial components in Chinese hamster ovary/CD14 cells is not affected by overexpression of Toll-like receptors 2 or 4.
    J Immunol. 167: 2257-67.
  7. Bieback, K. et al. (2002) Hemagglutinin protein of wild-type measles virus activates toll-like receptor 2 signaling.
    J Virol. 76: 8729-36.
  8. Karlsson, H. et al. (2002) Innate immune responses of human neonatal cells to bacteria from the normal gastrointestinal flora.
    Infect Immun. 70: 6688-96.
  9. View The Latest Product References
  10. Devaney, J.M. (2003) Neutrophil elastase up-regulates interleukin-8 via toll-like receptor 4.
    FEBS Lett. 544:129-32.
  11. Elner, S.G. et al. (2005) TLR4 mediates human retinal pigment epithelial endotoxin binding and cytokine expression.
    Invest Ophthalmol Vis Sci. 46 (12): 4627-33.
  12. Cuschieri, J. et al. (2006) Endotoxin tolerance attenuates LPS-induced TLR4 mobilization to lipid rafts: a condition reversed by PKC activation.
    J Leukoc Biol. 80: 1289-97.
  13. Lindsay, J.O. et al. (2006) Clinical, microbiological, and immunological effects of fructo-oligosaccharide in patients with Crohn's disease.
    Gut. 55: 348-55.
  14. Foster, N. et al. (2007) Pivotal advance: vasoactive intestinal peptide inhibits up-regulation of human monocyte TLR2 and TLR4 by LPS and differentiation of monocytes to macrophages.
    J Leukoc Biol. 81: 893-903.
  15. Pioli, P.A. et al. (2007) Estradiol attenuates lipopolysaccharide-induced CXC chemokine ligand 8 production by human peripheral blood monocytes.
    J Immunol. 179: 6284-90.
  16. Ibeagha-Awemu, E.M. et al. (2008) Bacterial lipopolysaccharide induces increased expression of toll-like receptor (TLR) 4 and downstream TLR signaling molecules in bovine mammary epithelial cells.
    Vet Res. 39 (2): 11.
  17. Chochi, K. et al. (2008) Helicobacter pylori augments growth of gastric cancers via the lipopolysaccharide-toll-like receptor 4 pathway whereas its lipopolysaccharide attenuates antitumor activities of human mononuclear cells.
    Clin Cancer Res. 14 (10): 2909-17.
  18. Brännström, K. et al. (2009) The Schistosoma mansoni protein Sm16/SmSLP/SmSPO-1 assembles into a nine-subunit oligomer with potential To inhibit Toll-like receptor signaling.
    Infect Immun. 77: 1144-54.
  19. de Kleer, I. (2010) CD30 Discriminates Heat Shock Protein 60-Induced FOXP3+CD4+ T Cells with a Regulatory Phenotype.
    J Immunol. 185(4):2071-9.
  20. Garbe, K. et al. (2012) Plasmacytoid dendritic cells and their Toll-like receptor 9 expression selectively decrease with age.
    Hum Immunol. 73 (5): 493-7.
  21. Komori, H. et al. (2012) α(1)-Acid glycoprotein up-regulates CD163 via TLR4/CD14 protein pathway: possible protection against hemolysis-induced oxidative stress.
    J Biol Chem. 287 (36): 30688-700.
  22. Prokhorenko, I. et al. (2012) Toll-like receptor 4 in phagocytosis of Escherichia coli by endotoxin-activated human neutrophils in whole blood
    Crit Care 16 (Suppl 3), P80
  23. Sels, J.W. et al. (2012) Fractional flow reserve is not associated with inflammatory markers in patients with stable coronary artery disease.
    PLoS One. 7: e46356.
  24. Maiolini, A. et al. (2012) Toll-like receptors 4 and 9 are responsible for the maintenance of the inflammatory reaction in canine steroid-responsive meningitis-arteritis, a large animal model for neutrophilic meningitis.
    J Neuroinflammation. 9: 226.
  25. Kyrova, K. et al. (2014) The response of porcine monocyte derived macrophages and dendritic cells to Salmonella typhimurium and lipopolysaccharide.
    BMC Vet Res. 10: 244.
  26. Zwolak, A. et al. (2015) Hyperreactivity of Blood Leukocytes in Patients with NAFLD to ex vivo Lipopolysaccharide Treatment Is Modulated by Metformin and Phosphatidylcholine but Not by Alpha Ketoglutarate.
    PLoS One. 10 (12): e0143851.
  27. Xu, H. et al. (2015) Type 3 innate lymphoid cell depletion is mediated by TLRs in lymphoid tissues of simian immunodeficiency virus-infected macaques.
    FASEB J. 29 (12): 5072-80.
  28. Blagitz, M,G. et al. (2015) Expression of CD14 and toll-like receptors 2 and 4 by milk neutrophils in bovine mammary glands infected with Corynebacterium bovis
    Pesquisa Veterinária Brasileira. 35 (1): 1-5.
  29. Mazzucchelli, I. et al. (2015) Expression and function of toll-like receptors in human circulating endothelial colony forming cells.
    Immunol Lett. 168 (1): 98-104.
  30. Zwolak, A. et al. (2016) Metformin Changes the Relationship between Blood Monocyte Toll-Like Receptor 4 Levels and Nonalcoholic Fatty Liver Disease-Ex Vivo Studies.
    PLoS One. 11 (3): e0150233.
  31. Huang, D. et al. (2016) Hyperoxia induces inflammation and regulates cytokine production in alveolar epithelium through TLR2/4-NF-κB-dependent mechanism
    Eur Rev Med Pharmacol Sci. 20: 1399-410.
  32. Reineking, W. et al. (2018) Canine primary jejunal and colonic epithelial cells predominantly express TLR5 and TLR9 but do not change TLR expression pattern after stimulation with certain Toll-like receptor ligands.
    Vet Immunol Immunopathol. 206: 16-24.
  33. Awuah, D. et al. (2019) The Cross-Talk between miR-511-3p and C-Type Lectin Receptors on Dendritic Cells Affects Dendritic Cell Function.
    J Immunol. 203 (1): 148-57.

Flow Cytometry

Functional Assays

Western Blotting

Synonyms
TLR4
RRID
AB_324150
UniProt
O00206
Entrez Gene
TLR4

MCA2061A647T

151524

MCA2061A647

1702

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