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CD8 Alpha antibody | CA9.JD3

Product Code Applications Pack Size List Price Your Price Qty
MCA1999S
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SDS Safety Datasheet SDS
C F IP 2 ml loader
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loader

Mouse anti Dog CD8 alpha antibody, clone CA9.JD3 recognizes the canine CD8 alpha chain which is expressed by thymocytes, peripheral T cells in the blood and lymphoid organs .

Mouse anti Dog CD8α clone CA9.JD3 has been reported to inhibit cytotoxic T lymphocyte function (Cobbold et al. 1994). CA9.JD3 immunoprecipitates a heterodimer of ~32 kDa and ~36 kDa (reduced) from canine Tumor cells and from thymocytes.

Target Species
Dog
Product Form
Tissue culture supernatant - liquid
Preservative Stabilisers
<0.1% sodium azide (NaN3)
Immunogen
Canine thymocytes.
Fusion Partners
Spleen cells from immunized Balb/c mice were fused with cells of the P3X63-Ag.653 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Immunohistology - Frozen Neat 1/10
Immunoprecipitation
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10μl of the suggested working dilution to label 106 cells in 100μl
Histology Positive Control Tissue
Canine spleen, lymph node

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Source Reference

  1. Moore, P.F. et al. (1992) Monoclonal antibodies specific for canine CD4 and CD8 define functional T-lymphocyte subsets and high-density expression of CD4 by canine neutrophils.
    Tissue Antigens 40(2): 75-85.

References for CD8 Alpha antibody

  1. Cobbold, S. & Metcalfe, S. (1994) Monoclonal antibodies that define canine homologues of human CD antigens: summary of the First International Canine Leukocyte Antigen Workshop (CLAW).
    Tissue Antigens. 43 (3): 137-54.
  2. Yuasa, K. et al. (2007) Injection of a recombinant AAV serotype 2 into canine skeletal muscles evokes strong immune responses against transgene products.
    Gene Ther. 14: 1249-60.
  3. Veenhof, E.Z. et al. (2011) Characterisation of T cell phenotypes, cytokines and transcription factors in the skin of dogs with cutaneous adverse food reactions.
    Vet J. 187 (3): 320-4.
  4. Lin Shiow-Chen et al. (2014) Immune Characterization of Peripheral Blood Mononuclear cells of the Dogs Restored from Innoculation of Canine Transmissible Venereal Tumor Cells.
    Tai Vet J. 40 (04): 181-90.
  5. Constantinoiu, C.C. et al. (2015) Mucosal tolerance of the hookworm Ancylostoma caninum in the gut of naturally infected wild dogs.
    Parasite Immunol. 37 (10): 510-20.
  6. Huyghe, S. et al. (2016) The Microscopic Structure of the Omentum in Healthy Dogs: The Mystery Unravelled.
    Anat Histol Embryol. 45 (3): 209-18.
  7. Knebel, A. et al. (2021) Measurement of canine Th17 cells by flow cytometry.
    Vet Immunol Immunopathol. 243: 110366.
  8. Bertolo, P.H.L. et al. (2022) Influence of serum progesterone levels on the inflammatory response of female dogs with visceral leishmaniosis.
    Vet Parasitol. 302: 109658.
  9. View The Latest Product References
  10. Wesolowski, M. et al. (2023) Long-term changes of Th17 and regulatory T cells in peripheral blood of dogs with spinal cord injury after intervertebral disc herniation.
    BMC Vet Res. 19 (1): 90.

Immunohistology - Frozen

RRID
AB_323370
UniProt
P33706
Entrez Gene
CD8A
GO Terms
GO:0016021 integral to membrane

MCA1999S

165697 180425

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