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CD62E antibody | CL2/6

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Anti Human CD62E Antibody, clone CL2/6 thumbnail image 1
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Mouse anti Human CD62e antibody, clone CL2/6 (MCA1969) used to affect CHO-P cell adhesion to selectin bearing substrate under shear stress conditions.
Image caption:
MSC-PPG-19Fc[FUT7+] binding to P- and E-selectin in microfluidic flow chamber A-B. 2-5×106 MSCs/mL immobilized with either 100 μg/mL 19Fc[FUT7+], 100-200 μg/mL anti-P-selectin mAb G1 or 100-200 μg/mL anti-E-selectin mAb CL2/6 were perfused over either P-selectin bearing CHO-P cells at a wall shear stress of 1 dyn/cm2 (panel A) or E-selectin bearing stimulated HUVECs at 2 dyn/cm2 (panel B). Only the MSC-PPG-19Fc[FUT7+] cells bound the selectin bearing substrate. Cell interaction was specifically blocked using anti-PSGL-1 mAb KPL1, anti-P-selectin mAb G1 or anti-E-selectin mAb CL2/6 as indicated. Data are mean ± SD for 3-4 independent experiments. * indicates P <0.001 with respect to all other conditions. C. Cumulative rolling velocity plot showing median rolling velocity of 3 μm/s when MSC-PPG-19Fc[FUT7+] roll on P-selectin/CHO-P cells at 1 dyn/cm2 and 11.5 μm/s for rolling on E-selectin/ HUVECs at 2 dyn/cm2. The rolling characteristics of 190 cells were analyzed and binned from three independent runs.

From: Lo CY, Antonopoulos A, Dell A, Haslam SM, Lee T, Neelamegham S.
The use of surface immobilization of P-selectin glycoprotein ligand-1 on mesenchymal stem cells to facilitate selectin mediated cell tethering and rolling.
Biomaterials. 2013 Nov;34(33):8213-22.

Mouse anti Human CD62E:FITC

Product Type
Monoclonal Antibody
Clone
CL2/6
Isotype
IgG2a
Specificity
CD62E

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Product Code Applications Pack Size List Price Your Price Qty
MCA1969F
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 100 Tests loader
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Mouse anti Human CD62E antibody, clone CL2/6 recognizes human CD62E, a member of the selectin family which is expressed on endothelial cells at sites of inflammation.

Mouse anti Human CD62E antibody, clone CL2/6 has been shown to inhibit the binding of human neutrophils to stimulated HUVECS.

Target Species
Human
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Preparation
Antibody purified from tissue culture supernatant
Buffer Solution
TRIS buffered saline
Preservative Stabilisers
0.02% sodium azide (NaN3)
1% bovine serum albumin
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
FITC 490 525
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10μl of the suggested working dilution to label 106 cells in 100μl

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Description Mouse IgG2a Negative Control:FITC

Description Product Code Applications Pack Size List Price Your Price Quantity
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References for CD62E antibody

  1. Kishimoto, T.K. et al. (1991) Antibodies against human neutrophil LECAM-1 (LAM-1/Leu-8/DREG-56 antigen) and endothelial cell ELAM-1 inhibit a common CD18-independent adhesion pathway in vitro.
    Blood. 78: 805-11.
  2. Abbassi, O. et al. (1993) E-selectin supports neutrophil rolling in vitro under conditions of flow.
    J Clin Invest. 92 (6): 2719-30.
  3. Nieuwland, R. et al. (2000) Cellular origin and procoagulant properties of microparticles in meningococcal sepsis.
    Blood. 95: 930-5.
  4. Rius, C. et al. (2010) Trans- but not cis-resveratrol impairs angiotensin-II-mediated vascular inflammation through inhibition of NF-κB activation and peroxisome proliferator-activated receptor-gamma upregulation.
    J Immunol. 185: 3718-27.
  5. Marin, C. et al. (2011) Mediterranean diet reduces endothelial damage and improves the regenerative capacity of endothelium.
    Am J Clin Nutr. 93: 267-74.
  6. Wassmer, S.C. et al. (2011) Vascular endothelial cells cultured from patients with cerebral or uncomplicated malaria exhibit differential reactivity to TNF.
    Cell Microbiol. 13: 198-209.
  7. Lo. C. Y. et al. (2013) The use of surface immobilization of P-selectin glycoprotein ligand-1 on mesenchymal stem cells to facilitate selectin mediated cell tethering and rolling
    Biomaterials. 34 (33): 8213-22.

Flow Cytometry

Synonyms
E-SELECTIN
RRID
AB_323219
UniProt
P16581
Entrez Gene
SELE
GO Terms
GO:0007157 heterophilic cell-cell adhesion
GO:0002092 positive regulation of receptor internalization
GO:0002523 leukocyte migration involved in inflammatory response
GO:0016021 integral to membrane
GO:0004888 transmembrane receptor activity
GO:0005615 extracellular space
GO:0005901 caveola
GO:0005905 coated pit
GO:0007202 activation of phospholipase C activity
GO:0019722 calcium-mediated signaling
GO:0030029 actin filament-based process
GO:0030863 cortical cytoskeleton
GO:0032496 response to lipopolysaccharide
GO:0033691 sialic acid binding
GO:0034612 response to tumor necrosis factor
GO:0043274 phospholipase binding
GO:0048471 perinuclear region of cytoplasm
GO:0050727 regulation of inflammatory response
GO:0050901 leukocyte tethering or rolling
GO:0070492 oligosaccharide binding
GO:0070555 response to interleukin-1

MCA1969F

030915A 166696

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