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CD200 antibody | OX-104

Product Code Applications Pack Size List Price Your Price Qty
MCA1960
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F 0.2 mg loader
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loader
MCA1960T
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F 25 µg loader
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loader
MCA1960GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F 0.1 mg loader
List Price Your Price
loader

Mouse anti Human CD200 antibody, clone OX-104 recognizes the human CD200 cell surface antigen, also known as OX2.

CD200 is expressed by a subset of B lymphocytes, some endothelial cells and by neurons. The CD200-CD200 ligand system is of importance in the control of macrophage and granulocyte activation.

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% sodium azide (NaN3)
Carrier Free
Yes
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/50 1/100
Immunohistology - Frozen
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10μl of the suggested working dilution to label 106 cells in 100μl

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Description Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (Fc):FITC
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Description Goat anti Mouse IgG (Fc):HRP
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Description Rabbit F(ab')2 anti Mouse IgG:RPE
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Description Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)
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Description Goat anti Mouse IgG:FITC (Rat Adsorbed)
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Description Goat anti Mouse IgG:RPE (Rat Adsorbed)
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Description Rabbit F(ab')2 anti Mouse IgG:FITC

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG1 Negative Control MCA928 F 100 Tests loader
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Description Mouse IgG1 Negative Control

References for CD200 antibody

  1. Wright, G.J. et al. (2001) The unusual distribution of the neuronal/lymphoid cell surface CD200 (OX2) glycoprotein is conserved in humans.
    Immunology 102 (2): 173-9.
  2. Raftery, M.J. et al. (2004) Shaping phenotype, function, and survival of dendritic cells by cytomegalovirus-encoded IL-10.
    J Immunol. 173: 3383-91.
  3. Ohyama, M. et al. (2006) Characterization and isolation of stem cell-enriched human hair follicle bulge cells.
    J Clin Invest. 116: 249-60.
  4. Koning, N. et al. (2007) Downregulation of macrophage inhibitory molecules in multiple sclerosis lesions.
    Ann Neurol. 62: 504-14.
  5. Kloepper, J.E. et al. (2008) Immunophenotyping of the human bulge region: the quest to define useful in situ markers for human epithelial hair follicle stem cells and their niche.
    Exp Dermatol. 17 (7): 592-609.
  6. Kloepper, J.E. et al. (2008) Immunophenotyping of the human bulge region: the quest to define useful in situ markers for human epithelial hair follicle stem cells and their niche.
    Exp Dermatol. 17: 592-609.
  7. Meuth, S.G. et al. (2008) CNS inflammation and neuronal degeneration is aggravated by impaired CD200-CD200R-mediated macrophage silencing.
    J Neuroimmunol. 194: 62-9.
  8. Koning, N. et al. (2009) Distribution of the immune inhibitory molecules CD200 and CD200R in the normal central nervous system and multiple sclerosis lesions suggests neuron-glia and glia-glia interactions.
    J Neuropathol Exp Neurol. 68: 159-67.
  9. View The Latest Product References
  10. Ko, Y.C. et al. (2009) Endothelial CD200 is heterogeneously distributed, regulated and involved in immune cell-endothelium interactions.
    J Anat. 214: 183-95.
  11. Yamauchi, K. and Kurosaka, A. (2010) Expression and function of glycogen synthase kinase-3 in human hair follicles.
    Arch Dermatol Res. 302: 263-70.
  12. Patel, G.K. et al. (2012) Identification and characterization of tumor-initiating cells in human primary cutaneous squamous cell carcinoma.
    J Invest Dermatol. 132 (2): 401-9.
  13. Ohyama, M. & Kobayashi, T. (2012) Isolation and characterization of stem cell-enriched human and canine hair follicle keratinocytes.
    Methods Mol Biol. 879: 389-401.
  14. Colmont, C.S. et al. (2013) CD200-expressing human basal cell carcinoma cells initiate tumor growth.
    Proc Natl Acad Sci U S A. 110 (4): 1434-9.
  15. Darmochwal-Kolarz, D. et al. (2013) The expressions of co-stimulatory molecules are altered on putative antigen-presenting cells in cord blood.
    Am J Reprod Immunol. 69 (2): 180-7.
  16. Chen, H.J. et al. (2015) Human placenta-derived adherent cells improve cardiac performance in mice with chronic heart failure.
    Stem Cells Transl Med. 4 (3): 269-75.
  17. Bertolini, M. et al. (2023) Mechanical epilation exerts complex biological effects on human hair follicles and perifollicular skin: An ex vivo study approach.
    Int J Cosmet Sci. Nov 03 [Epub ahead of print].

Flow Cytometry

Immunohistology - Frozen

Synonyms
OX2
RRID
AB_321839
UniProt
P41217
Entrez Gene
CD200
GO Terms
GO:0005887 integral to plasma membrane
GO:0050776 regulation of immune response

MCA1960

1605 165862 1804

MCA1960T

0410 152001

MCA1960GA

1804

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