IgE antibody | MARE-1
Mouse anti Rat IgE antibody, clone MARE-1 (MCA193) used to stimulate mast cells in vitro as a positive control prior to ELISA measurement of CCL3 production.
Image caption:
Effect of LL-37 on CCL3 production. For CCL3 measurement, mast cells were incubated with different concentrations of LL-37, anti-IgE at 5 μg/mL (positive control), or medium alone (NS) for 2 h. Results are the mean ± SD of four independent experiments (N=8). Comparisons between groups were carried out by using Student's t-test for small groups. Differences were considered significant at P < 0.05 and are labeled with an asterisk (*) on each graph.
From: Agier J, Brzezińska-Błaszczyk E, żelechowska P, Wiktorska M, Pietrzak J, Różalska S.
Cathelicidin LL-37 Affects Surface and Intracellular Toll-Like Receptor Expression in Tissue Mast Cells.
J Immunol Res. 2018 Feb 19;2018:7357162.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Rat IgE antibody, clone MARE1 (MCA193) used as a challenge in a cytokine release assay for the determination of multiple cytokine production values in vitro.
Image caption:
FcεRI-mediated release of de novo synthesized mediators upon rPMC stimulation with TLR3 ligand. rPMCs were treated with medium alone, poly(I:C) at 10 μg/mL, anti-IgE at 5 μg/mL, or both poly (I:C) and anti-IgE for 2 (a) or 12 h (b–f). CysLT (a), IFN-α (b), IFN-β (c), TNF (d), CCL3 (e), and CXCL8 (f) were measured in cell supernatants using ELISA. Bars for the poly(I:C) at 10 μg/mL and medium alone demonstrate the same data set as in Figure 3. Results are presented as the mean ± SD of 4 independent experiments, and each experiment was done in duplicate (n = 8). ∗p <0.05, ∗∗p <0.01, and ∗∗∗p <0.001; n.s.: not significant.
From: Witczak P, Brzezińska-Błaszczyk E, Agier J.
The Response of Tissue Mast Cells to TLR3 Ligand Poly(I:C) Treatment.
J Immunol Res. 2020 Feb 24;2020:2140694.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Rat IgE:FITC
- Product Type
- Monoclonal Antibody
- Clone
- MARE-1
- Isotype
- IgG1
- Specificity
- IgE
Antibody Quality and Performance Guarantee
| Mouse anti Rat IgE antibody, clone MARE-1 recognizes rat epsilon heavy chain of immunoglobulin IgE and does not cross-react with other classes of rat immunoglobulin. Mouse anti Rat IgE antibody, clone MARE-1 binds to rat IgE with an avidity of 4x109M-1 |
- Target Species
- Rat
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Preparation
- Purified IgG prepared by affinity chromatography from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.1% Sodium Azide 50% Glycerol - Immunogen
- Rat IR162, IR1016, IR2 and IR410 IgE myeloma proteins.
- Approx. Protein Concentrations
- IgG concentration 1 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line.
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) FITC 490 525 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
5ug/ml |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 50ul of the suggested working dilution to label 2 x 107 cells in 100ul.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control:FITC | MCA1209F | F | 0.1 mg |
|
Log in | ||
| List Price | Your Price | ||||||
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| Description | Mouse IgG1 Negative Control:FITC | ||||||
References for IgE antibody
-
Negrão-Corrêa, D. et al. (1996) Intestinal transport and catabolism of IgE: a major blood-independent pathway of IgE dissemination during a Trichinella spiralis infection of rats.
J Immunol. 157 (9): 4037-44. -
Silveira, M.R. et al. (2002) Infection with Strongyloides venezuelensis induces transient airway eosinophilic inflammation, an increase in immunoglobulin E, and hyperresponsiveness in rats.
Infect Immun. 70: 6263-72. -
Bąbolewska, E. & Brzezińska-błaszczyk, E. (2015) Human-derived cathelicidin LL-37 directly activates mast cells to proinflammatory mediator synthesis and migratory response.
Cell Immunol. 293 (2): 67-73. -
Korinek, M. et al. (2016) Anti-allergic potential of Typhonium blumei: inhibition of degranulation via suppression of PI3K/PLCγ2 phosphorylation and calcium influx.
Phytomedicine. 23 (14): 1706-15. -
Tulinská, J. et al. (2018) Humoral and cellular immune response in Wistar Han RCC rats fed two genetically modified maize MON810 varieties for 90 days (EU 7th Framework Programme project GRACE).
Arch Toxicol. 92 (7): 2385-99. -
Ueta, H. et al. (2018) Single blood transfusion induces the production of donor-specific alloantibodies and regulatory T cells mainly in the spleen.
Int Immunol. 30 (2): 53-67. -
Agier, J. et al. (2018) Cathelicidin LL-37 Affects Surface and Intracellular Toll-Like Receptor Expression in Tissue Mast Cells.
J Immunol Res. 2018: 7357162. -
Witczak, P. et al. (2020) The Response of Tissue Mast Cells to TLR3 Ligand Poly(I:C) Treatment.
J Immunol Res. 2020: 2140694.
View The Latest Product References
-
żelechowska, P. et al. (2022) Different effectiveness of fungal pathogen-associated molecular patterns (PAMPs) in activating rat peritoneal mast cells.
Immunol Lett. 248: 7-15. -
żelechowska, P. et al. (2021) Mannan activates tissue native and IgE-sensitized mast cells to proinflammatory response and chemotaxis in TLR4-dependent manner.
J Leukoc Biol. 109 (5): 931-42. -
żelechowska, P. et al. (2021) Native and IgE-primed rat peritoneal mast cells exert pro-inflammatory activity and migrate in response to yeast zymosan upon Dectin-1 engagement.
Immunol Res. 69 (2): 176-88.
Further Reading
-
Bazin, H. et al. (1974) Transplantable immunoglobulin-secreting tumours in rats. IV. Sixty-three IgE-secreting immunocytoma tumours.
Immunology. 26 (4): 713-23. -
Bazin, H. et al. (1978) Transplantable IgD immunoglobulin-secreting tumors in rat.
J Immunol. 121 (5): 2077-82. -
Bazin, H. et al. (1984) Rat monoclonal antibodies. II. A rapid and efficient method of purification from ascitic fluid or serum.
J Immunol Methods. 71 (1): 9-16. -
Bazin, H. et al. (1984) Rat monoclonal antibodies. I. Rapid purification from in vitro culture supernatants.
J Immunol Methods. 66 (2): 261-9.
- RRID
- AB_321901
- UniProt
- P01855
- Entrez Gene
- Ighe
- GO Terms
- GO:0003823 antigen binding
MCA193F
161132
180117
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