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CD147 antibody | MEM-M6/1

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Anti Human CD147 Antibody, clone MEM-M6/1 (Monoclonal Antibody Antibody) thumbnail image 1 Anti Human CD147 Antibody, clone MEM-M6/1 (Monoclonal Antibody Antibody) thumbnail image 2 Anti Human CD147 Antibody, clone MEM-M6/1 (Monoclonal Antibody Antibody) thumbnail image 3 Anti Human CD147 Antibody, clone MEM-M6/1 (Monoclonal Antibody Antibody) thumbnail image 4 Anti Human CD147 Antibody, clone MEM-M6/1 (Monoclonal Antibody Antibody) thumbnail image 5
Anti Human CD147 Antibody, clone MEM-M6/1 (Monoclonal Antibody Antibody) gallery image 1
Flow cytometric staining of human erythrocytes with Mouse anti Human CD147 (MCA1876T).
Anti Human CD147 Antibody, clone MEM-M6/1 (Monoclonal Antibody Antibody) gallery image 2
Flow cytometric staining of human erythrocytes with Mouse anti Human CD147:APC (MCA1876APC).
Anti Human CD147 Antibody, clone MEM-M6/1 (Monoclonal Antibody Antibody) gallery image 3
Western blot analysis of HepG2 human hepatocellular carcinoma whole cell lysate probed with Mouse anti Human CD147 antibody (MCA1876) followed by HRP conjugated Goat anti Mouse IgG, visualized by chemiluminescence.
Anti Human CD147 Antibody, clone MEM-M6/1 (Monoclonal Antibody Antibody) gallery image 4
Western blot analysis of CD147 knockout HEK293 and wild type HEK293 (WT) whole cell lysates probed with (A) Mouse anti CD147 antibody (MCA1876) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000 STAR207P) and (B) hFAB rhodamine anti-tubulin primary antibody (12004166) and visualized on the ChemiDoc MP with a (A) 43 second and (B) 30 second exposure.
Anti Human CD147 Antibody, clone MEM-M6/1 (Monoclonal Antibody Antibody) gallery image 5
Normalized signal intensity of CD147 from CRISPR knockout and wild type lysates. Signal intensity was normalized to the tubulin signal.

Mouse anti Human CD147:RPE

Product Type
Monoclonal Antibody
Clone
MEM-M6/1
Isotype
IgG1
Format
Purified
Specificity
CD147

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Product Code Applications Pack Size List Price Your Price Qty
MCA1876PE
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F 100 Tests loader
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Mouse anti Human CD147 antibody, clone MEM-M6/1 recognizes the human CD147 cell surface antigen, also known as Basigin, EMMPRIN and collagenase stimulatory factor. CD147 is a 385 amino acid single pass type 1 trans-membrane glycoprotein bearing 3 potential N-glycosylation sites, a single N-terminal (distal) Ig-like C2-type domain amd a more proximal Ig-like V-type domain in its extracellular region. Mouse anti Human CD147 antibody, clone MEM-M6/1 was raised and screened against recombinant extracellular region of huCD147 expressed in CHO cells, along with a number of other MEM-M6 clones. Epitope mapping indicates that clone MEM-M6/1 binds to an epitope within the distal Ig-like C-2 type domain. This is confirmed by flow cytometry using beads coated with constructs containing only the distal Ig-like or proximal Ig-like domains (Koch et al. 1999).

Mouse anti Human CD147 antibody, clone MEM-M6/1 binds to both un-stimulated and phytohemagglutinin (PHA) stimulated (activated) T lymphocytes with levels of CD147 enhanced following PHA stimulation. CD147 is also expressed by peripheral blood and endothelial cells as well as many cultured cells of hematopoietic and non-hematopoietic origin (Koch et al. 1999). In addition to clone MEM-M6/1 we are also able to offer the MEM-M6/6 clone derived from the same fusion which recognizes an epitope in the membrane proximal Ig-like V type domain and has inhibitory effects on CD3 induced T cell activation (Kock et al. 1999)		 PrecisionAb Antibodies

Target Species
Human
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution
Reconstitute with 1.0 ml distilled water
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% sodium azide (NaN3)
1% bovine serum albumin
5% sucrose
Immunogen
CD147Rg, consisting of the cDNA coding for the extracellular region of human CD147.
Fusion Partners
Spleen cells from immunized Balb/c mice were fused with SP2/0 mouse myeloma cells.
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
RPE 488nm laser 496 578
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Prior to reconstitution store at +4°C. Following reconstitution store at +4°C.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10μl of the suggested working dilution to label 106 cells or 100μl whole blood

How to Use the Spectraviewer

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  • Select combined or multi-laser view to visualize the spectra

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Description Mouse IgG1 Negative Control:RPE

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Source Reference

  1. Koch, C. et al. (1999) T cell activation-associated epitopes of CD147 in regulation of the T cell response, and their definition by antibody affinity and antigen density.
    Int Immunol. 11 (5): 777-86.

References for CD147 antibody

  1. Schneiderhan, W. et al. (2007) Pancreatic stellate cells are an important source of MMP-2 in human pancreatic cancer and accelerate tumor progression in a murine xenograft model and CAM assay.
    J Cell Sci. 120: 512-9.
  2. Yang, Y. et al. (2008) Cyclophilin A up-regulates MMP-9 expression and adhesion of monocytes/macrophages via CD147 signalling pathway in rheumatoid arthritis.
    Rheumatology (Oxford). 47: 1299-310.
  3. Schneiderhan, W. et al. (2009) CD147 silencing inhibits lactate transport and reduces malignant potential of pancreatic cancer cells in in vivo and in vitro models
    Gut. 58: 1391-8.
  4. Chen, Y. et al. (2009) Upregulation of HAb18G/CD147 in activated human umbilical vein endothelial cells enhances the angiogenesis.
    Cancer Lett. 278: 113-21.
  5. Hu, J. et al. (2010) Involvement of HAb18G/CD147 in T cell activation and immunological synapse formation.
    J Cell Mol Med. 14 (8): 2132-43.
  6. Huang, Z. et al. (2013) Overexpression of CD147 contributes to the chemoresistance of head and neck squamous cell carcinoma cells.
    J Oral Pathol Med. 42: 541-6.
  7. Bernard, S.C. et al. (2014) Pathogenic Neisseria meningitidis utilizes CD147 for vascular colonization.
    Nat Med. 20 (7): 725-31.
  8. Nassif, X. et al. (2015) Cd147 as receptor for pilus-mediated adhesion of meningococci to vascular endothelia.
    Patent Publication number US20150110806 A1
    9. View The Latest Product References
  9. Trakarnsanga, K. et al. (2017) An immortalized adult human erythroid line facilitates sustainable and scalable generation of functional red cells.
    Nat Commun. 8: 14750.

Flow Cytometry

Synonyms
Neurothelin
RRID
AB_323334
UniProt
P35613
Entrez Gene
BSG
GO Terms
GO:0000139 Golgi membrane
GO:0005515 protein binding
GO:0007596 blood coagulation
GO:0016021 integral to membrane
GO:0005537 mannose binding
GO:0006090 pyruvate metabolic process
GO:0007166 cell surface receptor linked signaling pathway
GO:0015129 lactate transmembrane transporter activity
GO:0042470 melanosome
GO:0050900 leukocyte migration

MCA1876PE

INN1702

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