CD163 antibody | EDHu-1
Mouse anti Human CD163:Alexa Fluor® 488
- Product Type
- Monoclonal Antibody
- Clone
- EDHu-1
- Isotype
- IgG1
- Specificity
- CD163
Mouse anti Human CD163 antibody, clone EDHu-1 recognizes the human CD163 cell surface antigen, a 130-140 kDa glycoprotein expressed by tissue macrophages. CD163 expression may be induced on monocytes by culture in dexamethasone. Clone EDHu-1 is reported to inhibit the binding of haptoglobin/hemoglobin to CD163 (Madsen et al. 2004).Truncation mutation analysis demonstrates binding of EDHu-1 occurs via the N-terminal region of CD163 containing the first three scavenger receptor, Cysteine-rich, SRCR domains the third domain being critical as, cleavage of this domain at the major cleavage site ASP-265 abrogates binding to the N-terminal fragment. |
Our CD163 (EDHu-1) Antibody has been referenced in >88 publications* *Based on June 2020 data from CiteAb's antibody search engine. |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Rhesus Monkey Sheep Pig Guinea Pig Bovine Cynomolgus monkey - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to Alexa Fluor 488 - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
1% bovine serum albumin - Immunogen
- Leucocytes harvested from the pleural cavity of patients with idiopathic spontaneous pneumothorax
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) Alexa Fluor®488 495 519 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | Neat |
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells in 100μl
Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
---|---|---|---|---|---|---|---|
Mouse IgG1 Negative Control:Alexa Fluor® 488 | MCA928A488 | F | 100 Tests/1ml | Log in | |||
List Price | Your Price | ||||||
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Description | Mouse IgG1 Negative Control:Alexa Fluor® 488 |
Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
---|---|---|---|---|---|---|---|
Human Seroblock | BUF070A | F | 50 Test | Log in | |||
List Price | Your Price | ||||||
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Description | Human Seroblock | ||||||
Human Seroblock | BUF070B | F | 200 Test | Log in | |||
List Price | Your Price | ||||||
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Description | Human Seroblock |
References for CD163 antibody
-
Kristiansen, M. et al. (2001) Identification of the haemoglobin scavenger receptor.
Nature. 409 (6817): 198-201. -
Asleh, R. et al. (2003) Genetically determined heterogeneity in hemoglobin scavenging and susceptibility to diabetic cardiovascular disease.
Circ Res. 92: 1193-200. -
Madsen, M. et al. (2004) Molecular characterization of the haptoglobin.hemoglobin receptor CD163. Ligand binding properties of the scavenger receptor cysteine-rich domain region.
J Biol Chem. 279 (49): 51561-7. -
Montes de Oca, M. et al. (2005) Skeletal muscle inflammation and nitric oxide in patients with COPD.
Eur Respir J. 26: 390-7. -
Wang, X. et al. (2006) Monocyte/macrophage and T-cell infiltrates in peritoneum of patients with ovarian cancer or benign pelvic disease.
J Transl Med. 4: 30. -
Martens JH et al. (2006) Differential expression of a gene signature for scavenger/lectin receptors by endothelial cells and macrophages in human lymph node sinuses, the primary sites of regional metastasis.
J Pathol. 208 (4): 574-89. -
Kim, W.K. et al. (2006) CD163 identifies perivascular macrophages in normal and viral encephalitic brains and potential precursors to perivascular macrophages in blood.
Am J Pathol. 168 (3): 822-34. -
Fabriek, B.O. et al. (2007) The macrophage CD163 surface glycoprotein is an erythroblast adhesion receptor.
Blood 109: 5223-9.
View The Latest Product References
-
Jensen, T.O. et al. (2009) Macrophage markers in serum and tumor have prognostic impact in American Joint Committee on Cancer stage I/II melanoma.
J Clin Oncol. 27: 3330-7. -
Grund, S. et al (2009) The microglial/macrophagic response at the tumour-brain border of invasive meningiomas.
Neuropathol Appl Neurobiol. 35: 82-8. -
Jorgensen, J.M. et al (2009) Expression level, tissue distribution pattern, and prognostic impact of vascular endothelial growth factors VEGF and VEGF-C and their receptors Flt-1, KDR, and Flt-4 in different subtypes of non-Hodgkin lymphomas.
Leuk Lymphoma. 50: 1647-60. -
Moreno, J.A. et al. (2009) The CD163-expressing macrophages recognize and internalize TWEAK: potential consequences in atherosclerosis.
Atherosclerosis. 207: 103-10. -
Boyle, J.J. et al. (2009) Coronary intraplaque hemorrhage evokes a novel atheroprotective macrophage phenotype.
Am J Pathol. 174: 1097-108. -
Moreno JA et al. (2010) Peripheral artery disease is associated with a high CD163/TWEAK plasma ratio.
Arterioscler Thromb Vasc Biol. 30 (6): 1253-62. -
Taus, N.S. et al. (2010) Sheep (Ovis aries) airway epithelial cells support ovine herpesvirus 2 lytic replication in vivo.
Vet Microbiol. 145: 47-53. -
Vinet-Oliphant, H. et al. (2010) Neurokinin-1 receptor (NK1-R) expression in the brains of SIV-infected rhesus macaques: implications for substance P in NK1-R immune cell trafficking into the CNS.
Am J Pathol. 177: 1286-97. -
Herrmann-Hoesing, L.M. (2010) Ovine progressive pneumonia virus capsid antigen as found in CD163- and CD172a-positive alveolar macrophages of persistently infected sheep.
Vet Pathol. 47: 518-28. -
Seeboth, J. et al. (2012) The fungal T-2 toxin alters the activation of primary macrophages induced by TLR-agonists resulting in a decrease of the inflammatory response in the pig.
Vet Res. 43: 35. -
Kong, L.Q. et al. (2013) The clinical significance of the CD163+ and CD68+ macrophages in patients with hepatocellular carcinoma.
PLoS One. 8 (3): e59771. -
Zhang, W. et al. (2013) Myeloid clusters are associated with a pro-metastatic environment and poor prognosis in smoking-related early stage non-small cell lung cancer.
PLoS One 8: e65121. -
Tang, Z. et al. (2013) Glucocorticoids Enhance CD163 Expression in Placental Hofbauer Cells.
Endocrinology 154: 471-82. -
Berglin, L. et al. (2014) In situ characterization of intrahepatic non-parenchymal cells in PSC reveals phenotypic patterns associated with disease severity.
PLoS One 9: e105375. -
Liu, J. et al. (2014) Evidence for mTOR pathway activation in a spectrum of epilepsy-associated pathologies.
Acta Neuropathol Commun. 2: 71. -
Baek, J.H. et al. (2014) Extracellular Hb enhances cardiac toxicity in endotoxemic guinea pigs: protective role of haptoglobin.
Toxins (Basel) 6: 1244-59. -
Micci, L, et al. (2014) CD4 depletion in SIV-infected macaques results in macrophage and microglia infection with rapid turnover of infected cells.
PLoS Pathog. 10: e1004467. -
Pirilä E et al. (2015) Macrophages modulate migration and invasion of human tongue squamous cell carcinoma.
PLoS One 10 (3): e0120895. -
Blair, T.C. et al. (2016) Immunopathology of Japanese macaque encephalomyelitis is similar to multiple sclerosis.
J Neuroimmunol. 291: 1-10. -
Arranz-Solís D et al. (2016) Systemic and local immune responses in sheep after Neospora caninum experimental infection at early, mid and late gestation.
Vet Res. 47 (1): 2. -
Lakritz, J R. et al. (2016) α4-Integrin Antibody Treatment Blocks Monocyte/Macrophage Traffic to, Vascular Cell Adhesion Molecule-1 Expression in, and Pathology of the Dorsal Root Ganglia in an SIV Macaque Model of HIV-Peripheral Neuropathy.
Am J Pathol. 186 (7): 1754-61. -
Fry, L.M. et al. (2016) East Coast Fever Caused by Theileria parva Is Characterized by Macrophage Activation Associated with Vasculitis and Respiratory Failure.
PLoS One 11 (5): e0156004. -
Wächter, C. et al. (2016) Loss of cerebellar neurons in the progression of lentiviral disease: effects of CNS-permeant antiretroviral therapy.
J Neuroinflammation. 13 (1): 272. -
Derricott, H. et al. (2016) Characterizing Villitis of Unknown Etiology and Inflammation in Stillbirth.
Am J Pathol. 186 (4): 952-61. -
Zhu, C. et al. (2017) Activation of CECR1 in M2-like TAMs promotes paracrine stimulation-mediated glial tumor progression.
Neuro Oncol. 19 (5): 648-59. -
Furukawa S et al. (2017) Interleukin-33 produced by M2 macrophages and other immune cells contributes to Th2 immune reaction of IgG4-related disease.
Sci Rep. 7: 42413. -
Mallard, J. et al. (2017) A method for obtaining simian immunodeficiency virus RNA sequences from laser capture microdissected and immune captured CD68+ and CD163+ macrophages from frozen tissue sections of bone marrow and brain.
J Immunol Methods. 442: 59-63. -
Farina, A. et al. (2017) Epstein-Barr virus lytic infection promotes activation of Toll-like receptor 8 innate immune response in systemic sclerosis monocytes.
Arthritis Res Ther. 19 (1): 39. -
Schultz, N. et al. (2017) Amylin alters human brain pericyte viability and NG2 expression.
J Cereb Blood Flow Metab. 37 (4): 1470-82. -
Boxberg, M. et al. (2018) PD-L1 and PD-1 and characterization of tumor-infiltrating lymphocytes in high grade sarcomas of soft tissue - prognostic implications and rationale for immunotherapy.
Oncoimmunology. 7 (3): e1389366. -
Waddell, L.A. et al. (2018) ADGRE1 (EMR1, F4/80) Is a Rapidly-Evolving Gene Expressed in Mammalian Monocyte-Macrophages.
Front Immunol. 9: 2246. -
Eligini, S. et al. (2019) Biological profile of monocyte-derived macrophages in coronary heart disease patients: implications for plaque morphology.
Sci Rep. 9 (1): 8680. -
Chen, J. et al. (2019) Generation of Pigs Resistant to Highly Pathogenic-Porcine Reproductive and Respiratory Syndrome Virus through Gene Editing of CD163.
Int J Biol Sci. 15 (2): 481-492. -
Zhao, S. et al. (2020) CD14+ monocytes and CD163+ macrophages correlate with the severity of liver fibrosis in patients with chronic hepatitis C
Experimental and Therapeutic Medicine. 20 (6): 1-1. -
Hayashi, K. et al. (2020) The Natural History of Spontaneously Occurred Endometriosis in Cynomolgus Monkeys by Monthly Follow-Up Laparoscopy for Two Years.
Tohoku J Exp Med. 251 (4): 241-53. -
Pestronk, A. (2020) Chronic Graft Versus Host Myopathies: Noninflammatory, Multi-Tissue Pathology With Glycosylation Disorders.
J Neuropathol Exp Neurol. 79 (1): 102-12. -
Palaiologou, E. et al. (2020) Human placental villi contain stromal macrovesicles associated with networks of stellate cells.
J Anat. 236 (1): 132-41. -
Frafjord, A. et al. (2020) Antibody combinations for optimized staining of macrophages in human lung tumours.
Scand J Immunol. 92 (1): e12889. -
Ströbel, S. et al. (2021) A 3D primary human cell-based in vitro model of non-alcoholic steatohepatitis for efficacy testing of clinical drug candidates.
Sci Rep. 11 (1): 22765. -
Ikezumi, Y. et al. (2021) Steroid treatment promotes an M2 anti-inflammatory macrophage phenotype in childhood lupus nephritis.
Pediatr Nephrol. 36 (2): 349-59. -
Edwards, J.H. et al. (2021) Integration and functional performance of a decellularised porcine superflexor tendon graft in an ovine model of anterior cruciate ligament reconstruction.
Biomaterials. 279: 121204. -
Kemmerer, C.L. et al. (2021) Cerebrospinal fluid cytokine levels are associated with macrophage infiltration into tumor tissues of glioma patients
BMC Cancer. 21(1):1108. -
Lehmann, M. et al. (2021) New onset of mainly guttate psoriasis after COVID-19 vaccination: a case report.
J Eur Acad Dermatol Venereol. 35 (11): e752-e755. -
Gonçalves, V.M. et al. (2021) Macrophage and Lymphocyte Infiltration Is Associated with Volumetric Tumor Size but Not with Volumetric Growth in the T&yyml;bingen Schwannoma Cohort.
Cancers (Basel). 13 (3): 466. -
Baumann, D. et al. (2021) p38 MAPK signaling in M1 macrophages results in selective elimination of M2 macrophages by MEK inhibition.
J Immunother Cancer.9 (7): e002319. -
da Silva M.C.M. et al. (2021) Inhibition of CSF1R, a receptor involved in microglia viability, alters behavioral and molecular changes induced by cocaine.
Sci Rep. 11 (1): 15989. -
Ma, H. et al. (2021) Structural comparison of CD163 SRCR5 from different species sheds some light on its involvement in porcine reproductive and respiratory syndrome virus-2 infection in vitro..
Vet Res. 52 (1): 97. -
Holsapple, J.S. et al. (2021) Low Intensity Shockwave Treatment Modulates Macrophage Functions Beneficial to Healing Chronic Wounds.
Int J Mol Sci. 22(15):7844. -
Galea, I. et al. (2022) Iron Deposition in the Brain After Aneurysmal Subarachnoid Hemorrhage.
Stroke. 53 (5): 1633-42. -
Bartalska, K. et al. (2022) A systematic characterization of microglia-like cell occurrence during retinal organoid differentiation.
iScience. 25 (7): 104580. -
Pooley, H.B. et al. (2022) Sheep vaccinated against paratuberculosis have increased levels of B cells infiltrating the intestinal tissue.
Vet Immunol Immunopathol. 252: 110482. -
Rabufetti, A. et al. (2022) New onset of sarcoidosis after COVID-19 infection.
J Eur Acad Dermatol Venereol. 36 (10): e756-e759. -
Vafaee, T. et al. (2022) Repopulation of decellularised porcine pulmonary valves in the right ventricular outflow tract of sheep: Role of macrophages.
J Tissue Eng. 13: 20417314221102680. -
Albrecht, E. et al. (2023) Identification and Quantification of Proliferating Cells in Skeletal Muscle of Glutamine Supplemented Low- and Normal-Birth-Weight Piglets
Cells. 12 (4): 580. -
Park, S.M. et al. (2023) Seven-colour multiplex immunochemistry/immunofluorescence and whole slide imaging of frozen sections.
J Immunol Methods. 518: 113490. -
Wu, H. et al. (2023) Electrical stimulation of piezoelectric BaTiO3 coated Ti6Al4V scaffolds promotes anti-inflammatory polarization of macrophages and bone repair via MAPK/JNK inhibition and OXPHOS activation.
Biomaterials. 293: 121990. -
Lin, C. et al. (2023) Macrophage-like rapid uptake and toxicity of tattoo ink in human monocytes.
Immunology. Nov 14 [Epub ahead of print]. -
Gabutti, M. et al. (2023) Dimethyl Fumarate Used as an Effective Treatment for Granuloma Annulare Disseminatum: An Immunohistochemical Case Study.
Int J Mol Sci. 24 (17): 13355 -
White, K.S. et al. (2023) Simian immunodeficiency virus-infected rhesus macaques with AIDS co-develop cardiovascular pathology and encephalitis.
Front Immunol. 14: 1240946. -
Medina, J.P. et al. (2023) MSC therapy ameliorates experimental gouty arthritis hinting an early COX-2 induction.
Front Immunol. 14: 1193179.
- UniProt
- Q86VB7
- Entrez Gene
- CD163
- GO Terms
- GO:0005515 protein binding
- GO:0006953 acute-phase response
- GO:0005576 extracellular region
- GO:0005044 scavenger receptor activity
- GO:0005887 integral to plasma membrane
MCA1853A488
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