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CD81 antibody | Eat2

Product Code Applications Pack Size List Price Your Price Qty
MCA1846
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C * E F IP WB * 0.25 mg loader
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loader
MCA1846T
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C * E F IP WB * 25 µg loader
List Price Your Price
loader
MCA1846GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C * E F IP WB * 0.1 mg loader
List Price Your Price
loader

Hamster anti Mouse CD81 antibody, clone Eat2 recognizes mouse and rat CD81, also known as TAPA-1 or Target of the antiproliferative antibody 1. CD81 is a 236 amino acid ~26 kDa multipass transmembrane protein belonging to the TM4SF family (UniProt: P35762). In rodents CD81 is expressed at much higher levels on resting B cells than on T cells, although increased expression on T cells is found following activation. Hamster anti Mouse CD81 antibody, clone Eat2 induces homotypic aggregation of B cells and inhibits anti Ig and IL-4 induced proliferation (Maecker et al. 2000). Eat 2 requires the presence of both extracellular loops of TAPA-1 for binding.

Mice lacking CD81 demonstrate reduced fertility through impaired oocyte-sperm fusion, double knockout CD81-/- CD9-/- mice are completely infertile suggesting complimentary roles in oocyte-sperm fusion (Rubenstein et al. 2006).

Target Species
Mouse
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% sodium azide (NaN3)
Carrier Free
Yes
Immunogen
38C13, murine B cell line.
Approx. Protein Concentrations
MCA1846, MCA1846T: IgG concentration 1.0 mg/ml
MCA1846GA: IgG concentration 1 mg/ml
Fusion Partners
Spleen cells from immunised Armenian hamsters were fused with cells of the mouse PX3-Ag.8.653 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Flow Cytometry 1/50 Pack Size: 0.25 mg, 25 µg
1/10 Pack Size: 0.1 mg
1/100 Pack Size: 0.25 mg, 25 µg
1/50 Pack Size: 0.1 mg
Immunohistology - Frozen 1
Immunohistology - Paraffin
Immunoprecipitation
Western Blotting 2
  1. 1The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
  2. 2Clone Eat2 recognizes mouse CD81 under non-reducing conditions.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10μl of the suggested working dilution to label 106 cells in 100μl

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat F(ab')2 anti Hamster IgG:DyLight®550 STAR104D550 F IF WB 0.1 mg loader
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Description Goat F(ab')2 anti Hamster IgG:DyLight®550
Goat F(ab')2 anti Hamster IgG:DyLight®650 STAR104D650 F IF 0.1 mg loader
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Description Goat F(ab')2 anti Hamster IgG:DyLight®650
Goat F(ab')2 anti Hamster IgG:Dylight®800 STAR104D800GA F IF WB 0.1 mg loader
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Description Goat F(ab')2 anti Hamster IgG:Dylight®800
Goat F(ab')2 anti Hamster IgG:FITC STAR104F F 0.4 mg loader
List Price Your Price
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Description Goat F(ab')2 anti Hamster IgG:FITC
Goat anti Hamster IgG:Biotin STAR79B E F 0.8 mg loader
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Description Goat anti Hamster IgG:Biotin
Goat anti Hamster IgG:FITC STAR79F F 0.7 mg loader
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Description Goat anti Hamster IgG:FITC
Goat anti Hamster IgG:HRP STAR79P E 0.8 mg loader
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Description Goat anti Hamster IgG:HRP

References for CD81 antibody

  1. Maecker, H.T. et al. (2000) Differential expression of murine CD81 highlighted by new anti-mouse CD81 monoclonal antibodies.
    Hybridoma 19: 15-22.
  2. Owens, D.M. and Watt, F.M. (2001) Influence of beta1 integrins on epidermal squamous cell carcinoma formation in a transgenic mouse model: alpha3beta1, but not alpha2beta1, suppresses malignant conversion.
    Cancer Res. 61: 5248-54.
  3. Clark, K.L. et al. (2001) PGRL is a major CD81-associated protein on lymphocytes and distinguishes a new family of cell surface proteins.
    J Immunol. 167 (9): 5115-21.
  4. Ha, C.T. et al. (2005) Binding of pregnancy-specific glycoprotein 17 to CD9 on macrophages induces secretion of IL-10, IL-6, PGE2, and TGF-beta1.
    J Leukoc Biol. 77: 948-57.
  5. Takeda, Y. et al. (2008) Double deficiency of tetraspanins CD9 and CD81 alters cell motility and protease production of macrophages and causes chronic obstructive pulmonary disease-like phenotype in mice.
    J Biol Chem. 283: 26089-97.
  6. Conde-Vancells, J. et al. (2008) Characterization and comprehensive proteome profiling of exosomes secreted by hepatocytes.
    J Proteome Res. 7: 5157-66.
  7. Suzuki, M. et al. (2009) Tetraspanin CD9 negatively regulates lipopolysaccharide-induced macrophage activation and lung inflammation.
    J Immunol. 182: 6485-93.
  8. Conde-Vancells, J. et al. (2010) Candidate biomarkers in exosome-like vesicles purified from rat and mouse urine samples.
    Proteomics Clin Appl. 4 (4): 416-25.
  9. View The Latest Product References
  10. Pan, Q. et al. (2011) Hepatic cell-to-cell transmission of small silencing RNA can extend the therapeutic reach of RNA interference (RNAi).
    Gut. 61: 1330-9.
  11. Sosa, L.J. et al. (2013) Amyloid precursor protein is an autonomous growth cone adhesion molecule engaged in contact guidance.
    PLoS One. 8 (5): e64521.
  12. Royo, F. et al. (2013) Transcriptome of extracellular vesicles released by hepatocytes.
    PLoS One. 8: e68693.
  13. Jin, Y. et al. (2013) Statins decrease lung inflammation in mice by upregulating tetraspanin CD9 in macrophages.
    PLoS One. 8: e73706.
  14. Jin, Y. et al. (2018) Double deletion of tetraspanins CD9 and CD81 in mice leads to a syndrome resembling accelerated aging.
    Sci Rep. 8 (1): 5145.

Western Blotting

Synonyms
TAPA-1
RRID
AB_323200
UniProt
P35762
Entrez Gene
Cd81
GO Terms
GO:0000187 activation of MAPK activity
GO:0005515 protein binding
GO:0008104 protein localization
GO:0005887 integral to plasma membrane
GO:0006661 phosphatidylinositol biosynthetic process
GO:0030307 positive regulation of cell growth
GO:0030890 positive regulation of B cell proliferation
GO:0043128 positive regulation of 1-phosphatidylinositol 4-kinase activity
GO:0050731 positive regulation of peptidyl-tyrosine phosphorylation

MCA1846

149529

MCA1846T

149350 1709

MCA1846GA

149596 161759 1703

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